Coagulation Factor III/Tissue Factor Antibody

Western Blot: Coagulation Factor III/Tissue Factor Antibody [NBP2-15139] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Coagulation factor III/Tissue Factor antibody [N1C3]diluted at 1:1000.

Immunohistochemistry-Paraffin: Coagulation Factor III/Tissue Factor Antibody [NBP2-15139] - Human endometrial carcinoma. Coagulation factor III/Tissue Factor stained by Coagulation factor III/Tissue Factor antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: Coagulation Factor III/Tissue Factor Antibody [NBP2-15139] - Tissue Factor expression in paraffin-embedded human lung cancer tissues using Tissue Factor antibody. Image from verified customer review.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Coagulation Factor III/Tissue Factor Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Coagulation Factor III/Tissue Factor. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: F3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100 - 1:1000
  • Immunohistochemistry-Paraffin 1:100 - 1:1000
  • Western Blot 1:500 - 1:3000
• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Coagulation Factor III/Tissue Factor Antibody

• CD142 antigen
• CD142
• coagulation factor III (thromboplastin, tissue factor)
• Coagulation Factor III
• F3
• FLJ17960
• TF
• TFA
• Thromboplastin
• Tissue Factor

Background

This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Coagulation Factor III/Tissue Factor Antibody (NBP2-15139)(3)

Publications using NBP2-15139

• Ryan TAJ, Hooftman A, Rehill AM et al. Dimethyl fumarate and 4-octyl itaconate are anticoagulants that suppress Tissue Factor in macrophages via inhibition of Type I Interferon Nature communications 2023-06-14 [PMID: 37316487] (Immunohistochemistry, Mouse)
• Rosell A, Moser B, Hisada Y et al. Evaluation of different commercial antibodies for their ability to detect human and mouse tissue factor by western blotting Res Pract Thromb Haemost 2020-07-06 [PMID: 32864552] (WB, Human)
• Sommerville L. J, Gorman K. L, et al. A unique protein kinase C-dependent pathway for tissue factor downregulation in pericytes. J Thromb Haemost 2019-04-01 [PMID: 30698330] (WB, Human)

Review for Coagulation Factor III/Tissue Factor Antibody (NBP2-15139) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Christopher England IHC-P Human 09/03/2016

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Human lung cancer tissue samples
• Species: Human
• Lot: 41829

FAQs for Coagulation Factor III/Tissue Factor Antibody (NBP2-15139). (Showing 1 - 1 of 1 FAQs).

1. Have you tested any of your anti-tissue factor antibodies against pig? I need one for western blot.
   • Unfortunately, none of our Tissue Factor antibodies have yet been tested with pig samples, so we cannot guarantee that any of them will work in pig. Here is a link to Tissue Factor antibodies that have been validated for use in Western blot. If you are interested in testing any of these antibodies against pig samples, you could become eligible for our Innovator's Reward program.

Bioinformatics

• Gene Symbol: F3
• Uniprot:
  • P13726()