Collagen IV Antibody (COL4/4241R)

Immunohistochemistry-Paraffin: Collagen IV Antibody (COL4/4241R) [NBP3-07750] - Formalin-fixed, paraffin-embedded human tonsil stained with Collagen IV Recombinant Rabbit Monoclonal Antibody (COL4/4241R).

Immunohistochemistry-Paraffin: Collagen IV Antibody (COL4/4241R) [NBP3-07750] - Formalin-fixed, paraffin-embedded human tonsil stained with Collagen IV Recombinant Rabbit Monoclonal Antibody (COL4/4241R).

Formalin-fixed, paraffin-embedded human neuroendocrine tumor stained with Collagen IV antibody (COL4/4241R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human hemangiopericytoma stained with Collagen IV antibody (COL4/4241R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human kidney stained with Collagen IV antibody (COL4/4241R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Immunohistochemistry-Paraffin: Collagen IV Antibody (COL4/4241R) [NBP3-07750] - Formalin-fixed, paraffin-embedded human tonsil stained with Collagen IV antibody (COL4/4241R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

• Reactivity: Hu
• Applications: IHC
• Clone: COL4/4241R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Collagen IV Antibody (COL4/4241R) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08277)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Purified human placental extract
• Localization: Secreted. Extracellular matrix.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: COL4A1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 161 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Collagen IV Antibody (COL4/4241R)

• BSVD;BSVD1;COL4A1s;Collagen alpha-1(IV) chain;PADMAL;RATOR
• Collagen IV

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: COL4A1
• Uniprot:
  • P02462(Human)
  • P08572(Human)
  • P29400(Human)
  • P53420(Human)