![Immunohistochemistry-Paraffin: Collagen IV Antibody (COL4/4241R) - Azide and BSA Free [NBP3-08277] - Formalin-fixed, paraffin-embedded human tonsil stained with Collagen IV Recombinant Rabbit Monoclonal Antibody (COL4/4241R).](http://images.novusbio.com/fullsize/Collagen-IV-Antibody-COL4-4241R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08277-img0001.jpg "Immunohistochemistry-Paraffin: Collagen IV Antibody (COL4/4241R) - Azide and BSA Free [NBP3-08277] - Formalin-fixed, paraffin-embedded human tonsil stained with Collagen IV Recombinant Rabbit Monoclonal Antibody (COL4/4241R).")
| Reactivity | HuSpecies Glossary |
| Applications | IHC |
| Clone | COL4/4241R |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07750). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Purified human placental extract |
| Localization | Secreted. Extracellular matrix. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | COL4A1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 161 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Collagen IV Antibody (NBP3-08277)Find related products by research area.
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| Gene Symbol | COL4A1 |
![Immunohistochemistry-Paraffin: Collagen IV Antibody (COL4/4241R) - Azide and BSA Free [NBP3-08277] - Formalin-fixed, paraffin-embedded human tonsil stained with Collagen IV Recombinant Rabbit Monoclonal Antibody (COL4/4241R).](http://images.novusbio.com/fullsize/Collagen-IV-Antibody-COL4-4241R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08277-img0002.jpg "Immunohistochemistry-Paraffin: Collagen IV Antibody (COL4/4241R) - Azide and BSA Free [NBP3-08277] - Formalin-fixed, paraffin-embedded human tonsil stained with Collagen IV Recombinant Rabbit Monoclonal Antibody (COL4/4241R).")
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