Cortactin Antibody - BSA Free

Western Blot: Cortactin Antibody [NBP2-15971] - Wild-type (WT) and Cortactin knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1], N-term. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Cortactin Antibody [NBP2-15971] - Wild-type (WT) and Cortactin knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1], N-term. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Cortactin Antibody [NBP2-15971] - Sample (30 ug of whole cell lysate) A: PC-12 7. 5% SDS PAGE gel, diluted at 1:3000.

Immunohistochemistry-Paraffin: Cortactin Antibody [NBP2-15971] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.

Western Blot: Cortactin Antibody [NBP2-15971] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7. 5% SDS PAGE gel, diluted at 1:1000.

Immunohistochemistry-Paraffin: Cortactin Antibody [NBP2-15971] - Cortactin antibody [N1], N-term detects Cortactin protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.Cortactin stained by Cortactin antibody [N1], N-term (NBP2-15971) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunocytochemistry/ Immunofluorescence: Cortactin Antibody [NBP2-15971] - Cortactin antibody [N1], N-term detects Cortactin protein at cytoplasm and cell membrane by immunofluorescent analysis.Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cortactin stained by Cortactin antibody [N1], N-term (NBP2-15971) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .Scale bar= 10um.

Western Blot: Cortactin Antibody [NBP2-15971] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1], N-term (NBP2-15971) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Cortactin Antibody [NBP2-15971] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1], N-term (NBP2-15971) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, RM
• Applications: WB, ICC/IF, IHC, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Cortactin Antibody - BSA Free Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Cortactin. The exact sequence is proprietary.
• Localization: Cytoplasm; cytoskeleton (By similarity); Cell projection; lamellipodium (By similarity); ruffle (By similarity)
• Predicted Species: Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CTTN
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockout Validated
  • Western Blot 1:500-1:3000
• Theoretical MW: 62 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Cortactin Antibody - BSA Free

• 1110020L01Rik
• Amplaxin
• Cortactin
• CTTN
• ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 srcsubstrate)
• EMS1
• EMS1amplaxin
• FLJ34459
• Oncogene EMS1
• src substrate cortactin

Background

This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: CTTN
• Uniprot:
  • Q14247(Human)