COX-2 Antibody (SC56-06)

Western Blot: COX-2 Antibody (SC56-06) [NBP2-67566] - Western blot analysis of COX-2 on A549 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab

Immunocytochemistry/Immunofluorescence: COX-2 Antibody (SC56-06) [NBP2-67566] - Staining COX2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Western Blot: COX-2 Antibody (SC56-06) [NBP2-67566] - Analysis of COX2 on different lysates using anti-COX2 antibody at 1/1,000 dilution. Positive control: Lane 1: A549 Lane 2: THP-1

Immunocytochemistry/Immunofluorescence: COX-2 Antibody (SC56-06) [NBP2-67566] - Staining COX2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: COX-2 Antibody (SC56-06) [NBP2-67566] - Staining COX2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

COX-2 Antibody (SC56-06) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within human COX-2 aa 100-140. (SwissProt: P35354 Human; SwissProt: Q05769 Mouse; SwissProt: P35355 Rat)
• Localization: Microsome membrane, Endoplasmic reticulum membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PTGS2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry-Paraffin 1:100
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for COX-2 Antibody (SC56-06)

• COX2
• COX-2
• COX2cyclooxygenase 2b
• cyclooxygenase-2
• EC 1.14.99
• EC 1.14.99.1
• GRIPGHS
• hCox-2
• PGG/HS
• PGH synthase 2
• PGHS-2
• PHS II
• PHS-2
• PHS-II
• prostaglandin G/H synthase 2
• prostaglandin G/H synthase and cyclooxygenase
• Prostaglandin H2 synthase 2
• prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase andcyclooxygenase)
• Prostaglandin-endoperoxide synthase 2
• PTGS2

Background

COX2 (Cyclooxygenase 2) is an inducible enzyme. It is involved in the response of cells to growth factors, tumor promoters, and cytokines that induce its expression. Given its role in synthesizing prostaglandins, COX2 is therefore of interest in studying immune response regulation. COX2 is induced by a wide variety of stimuli and was initially identified as immediate early growth response gene. In addition, COX2 expression markedly increased in 85-90% of human colorectal adenocarcinoma whereas COX1 levels remain unchanged.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTGS2