Human COX-2 Luciferase - (LUCPorter™) Stable Reporter Cell Line

Product Discontinued

Human COX-2 Luciferase - (LUCPorter™) Stable Reporter Cell Line Summary

• Description: The COX2/LUCPoter(TM) reporter cell line is designed to monitor the induction of COX-2 and can be used for screening of agonists, antagonists or signaling inhibitors of COX-2 induction as well as for studying the COX-2 induction-related signaling pathways.
  
  Contents: 3~4 x 10^6 cells
  Biosafety Level: 2
• Immunogen: The COX2/LUCPorte(TM) reporter cell line is a stably transfected HEK 293 cell line which expresses an optimized Renilla luciferase reporter gene (RenSP) under the transcriptional control of the COX-2 promoter. Cyclooxygenase-2 (COX-2, prostaglandin endoperoxidase synthase-2) is an inducible enzyme of which expression levels are elevated during inflammation. COX2 has been an important human therapeutic target since its upregulation is involved in various disease processes such as inflammation, pain, fever, myocardial infarction, stroke, atheroma and diabetic retinopathy. Hence therapeutic inhibition of COX-2 can reduce disease progression including autoimmune disease, cardiovascular disease and cancer. COX-2 is induced by various stimuli or regulators including EGF, TGF, TNF, estrogen and androgen. The COX2/LUCPorte(TM) reporter cell line is a semi-constitutively active cell line and is also a stimuli-inducible cell line as shown in Figures 1 and 2.
• Target Species: Human
• Selection Agent: Puromycin at 3 ug/ml
• Growth Properties: Adherent
  Morphology : Epithelial
• RCL Type: Luciferase - (LUCPorter™)
• Host: HEK293
• Gene: PTGS2

Applications/Dilutions

• Dilutions:
  • Ligand Activation
• Readout System:
  • LightSwitch Luciferase Assay Reagent NBP2-32066

Packaging, Storage & Formulations

• Storage: Store in gas phase of liquid nitrogen.
• Reconstitution Instructions: Complete Growth Medium: DMEM with 4.5 g/L glucose + 10% FBS + 4 mM L-glutamine + 1 mM sodium pyruvate + 100 units/ml penicillin + 0.1 mg/ml streptomycin.

Alternate Names for Human COX-2 Luciferase - (LUCPorter™) Stable Reporter Cell Line

• COX2
• COX-2
• COX2cyclooxygenase 2b
• cyclooxygenase-2
• EC 1.14.99
• EC 1.14.99.1
• GRIPGHS
• hCox-2
• PGG/HS
• PGH synthase 2
• PGHS-2
• PHS II
• PHS-2
• PHS-II
• prostaglandin G/H synthase 2
• prostaglandin G/H synthase and cyclooxygenase
• Prostaglandin H2 synthase 2
• prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase andcyclooxygenase)
• Prostaglandin-endoperoxide synthase 2
• PTGS2

Background

COX2 (Cyclooxygenase 2) is an inducible enzyme. It is involved in the response of cells to growth factors, tumor promoters, and cytokines that induce its expression. Given its role in synthesizing prostaglandins, COX2 is therefore of interest in studying immune response regulation. COX2 is induced by a wide variety of stimuli and was initially identified as immediate early growth response gene. In addition, COX2 expression markedly increased in 85-90% of human colorectal adenocarcinoma whereas COX1 levels remain unchanged.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Reporter Cell Lines are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTGS2