DDX6 Antibody - BSA Free

Western Blot: DDX6 Antibody [NB200-192] - Detection of Human and Mouse DDX6 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DDX6 antibody NB200-192 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Immunohistochemistry-Paraffin: DDX6 Antibody [NB200-192] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-DDX6 (left) and Lot 3 (right) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).

Immunoprecipitation: DDX6 Antibody [NB200-192] - Detection of human DDX6 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DDX6 antibody NB200-192 (lot NB200-192-3) used for IP at 6 ug per reaction. DDX6 was also immunoprecipitated by a previous lot of this antibody (NB200-192-2) and rabbit anti-DDX6 antibody NB200-191. For blotting immunoprecipitated DDX6, NB200-192 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Western Blot: DDX6 Antibody [NB200-192] - Western blot analysis of HNRNPK and DDX6 protein levels upon HNRNPK or DDX6 knockdown. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-019-12238-x), licensed under a CC-BY license.

Immunocytochemistry/ Immunofluorescence: DDX6 Antibody [NB200-192] - 5-FU-induced SG assembly is rescued by interfering with RNA incorporation. HeLa cells were treated with 1 mM Urd, 0.1 mM 5-FU or both, as well as with 1 μM FUrd or a combination of 1 μM FUrd & 10 μM Urd. Localization of SG marker protein TIAR (green) & SG/P-body marker protein DDX6 (red) was investigated. Nuclei were stained with Hoechst. Scale bars represent 20 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24728989), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: DDX6 Antibody [NB200-192] - 5-FU-induced SG assembly depends on RNA incorporation. (A) Schematic representation of the cellular 5-FU metabolism leading to incorporation of the different metabolites into RNA or DNA. (B) HeLa cells were treated with two concentrations of the 5-FU metabolites FUrd or FdUrd for 72 h. SG marker protein TIAR (green) & SG/P-body marker protein DDX6 (red) were visualized. Nuclei were stained with Hoechst. Scale bars represent 20 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24728989), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: DDX6 Antibody [NB200-192] - HNRNPK is necessary for DDX6 to bind differentiation associated mRNAs. a RNA IP was performed in CTLi & HNRNPKi cells using a DDX6 antibody. RT-QPCR was used to determine the levels of binding between DDX6 & differentiation associated mRNAs in the presence or absence of HNRNPK. IGG IPs in CTLi & HNRNPKi cells were used as specificity controls. Binding was calculated as a percent of input. b RNA IP was performed in CTLi & DDX6i cells using a HNRNPK antibody. RT-QPCR was used to determine the levels of binding between HNRNPK & differentiation associated mRNAs in the presence or absence of DDX6. IGG IPs in CTLi & DDX6i cells were used as specificity controls. n = 4. c Western blot analysis of HNRNPK & DDX6 protein levels upon HNRNPK or DDX6 knockdown. d Immunoprecipitations (IPs) were performed using either an HNRNPK or DDX6 antibody or IGG & Western blotted for HNRNPK or DDX6 protein expression. IPs were performed +/− RNase A. Five percent of the cell lysate was used as input. Representative blots are shown. n = 3 independent experiments performed for Fig. 3 unless otherwise indicated. All error bars = SD. ****p < 0.0001, ***p < 0.001 (2 way ANOVA followed by Tukey’s multiple comparison test for a, b) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31519929), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: DDX6 Antibody [NB200-192] - HNRNPK is necessary for DDX6 to bind differentiation associated mRNAs. a RNA IP was performed in CTLi & HNRNPKi cells using a DDX6 antibody. RT-QPCR was used to determine the levels of binding between DDX6 & differentiation associated mRNAs in the presence or absence of HNRNPK. IGG IPs in CTLi & HNRNPKi cells were used as specificity controls. Binding was calculated as a percent of input. b RNA IP was performed in CTLi & DDX6i cells using a HNRNPK antibody. RT-QPCR was used to determine the levels of binding between HNRNPK & differentiation associated mRNAs in the presence or absence of DDX6. IGG IPs in CTLi & DDX6i cells were used as specificity controls. n = 4. c Western blot analysis of HNRNPK & DDX6 protein levels upon HNRNPK or DDX6 knockdown. d Immunoprecipitations (IPs) were performed using either an HNRNPK or DDX6 antibody or IGG & Western blotted for HNRNPK or DDX6 protein expression. IPs were performed +/− RNase A. Five percent of the cell lysate was used as input. Representative blots are shown. n = 3 independent experiments performed for Fig. 3 unless otherwise indicated. All error bars = SD. ****p < 0.0001, ***p < 0.001 (2 way ANOVA followed by Tukey’s multiple comparison test for a, b) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31519929), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Gp, Pm-Or
• Applications: WB, ICC/IF, IHC, IP, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

DDX6 Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residues 425 and the C-terminus (residue 483) of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 using the numbering given Swiss-Prot entry P26196 (GeneID 1656).
• Predicted Species: Orangutan (100%), Guinea Pig (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DDX6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:500 - 1:2-500
  • Immunohistochemistry 1:1000- 1:5000
  • Immunohistochemistry-Paraffin 1:1000- 1:5000
  • Immunoprecipitation 2-10 ug/mg lysate
  • Knockdown Validated
  • Western Blot 1:2000 - 1:10000
• Application Notes: Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections. ICC/IF reactivity reported in (PMID: 24728989).
• Control:

  DDX6 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DDX6 Antibody - BSA Free

• ATP-dependent RNA helicase p54
• DEAD (Asp-Glu-Ala-Asp) box polypeptide 6
• DEAD box protein 6
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD)
• EC 3.6.1
• EC 3.6.4.13
• FLJ36338
• HLR2DEAD box-6
• Oncogene RCK
• probable ATP-dependent RNA helicase DDX6
• RCKP54

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein. It may contribute to cell proliferation and carcinogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DDX6 Antibody (NB200-192)(27)

Publications using NB200-192

• Safieddine A, Benassy MN, Bonte T, Slimani F et Al. Cell-cycle-dependent mRNA localization in P-bodies Mol Cell 2024-10-05 [PMID: 39368464]
• Vukovic I, Barnada SM, Ruffin JW et Al. Non-redundant roles for the human mRNA decapping cofactor paralogs DCP1a and DCP1b Life Sci Alliance 2024-09-10 [PMID: 39256052]
• Iyer DP, Moyon L, Wittler L et Al. Combinatorial microRNA activity is essential for the transition of pluripotent cells from proliferation into dormancy Genome Res 2024-05-15 [PMID: 38719471]
• Perez-Perri JI, Ferring-Appel D, Huppertz I et al. The RNA-binding protein landscapes differ between mammalian organs and cultured cells Nature communications 2023-04-12 [PMID: 37045843] (PLA, Mouse)
• Cochard, A;Garcia-Jove Navarro, M;Piroska, L;Kashida, S;Kress, M;Weil, D;Gueroui, Z; RNA at the surface of phase-separated condensates impacts their size and number Biophysical journal [PMID: 35364105]
• Zanotti S, Vanhauwaert S, Van Neste C, et al. MYCN-induced nucleolar stress drives an early senescence-like transcriptional program in hTERT-immortalized RPE cells Sci Rep 2021-07-15 [PMID: 34262099]
• Choksupmanee O, Tangkijthavorn W, Hodge K et al. Specific Interaction of DDX6 with an RNA Hairpin in the 3'-UTR of the Dengue Genome Mediates G1 Phase Arrest Journal of Virology 2021-06-16 [PMID: 34132569]
• Furtado GV, Yang J, Wu D et al. FOXO1 controls protein synthesis and transcript abundance of mutant polyglutamine proteins, preventing protein aggregation Human molecular genetics 2021-04-02 [PMID: 33822053] (ICC/IF, Mouse)
• Damodaran AP, Courtheoux T, Watrin E, Prigent C Alteration of SC35 localization by transfection reagents Biochim Biophys Acta Mol Cell Res 2020-01-15 [PMID: 31953060] (ICC/IF, Human)
• Di Stefano B, Luo EC, Haggerty C et al. The RNA Helicase DDX6 Controls Cellular Plasticity by Modulating P-Body Homeostasis Cell Stem Cell 2019-09-27 [PMID: 31588046] (WB, ICC/IF, IP, KD, Mouse, Human)
• Li J, Chen Y, Xu X et al. HNRNPK maintains epidermal progenitor function through transcription of proliferation genes and degrading differentiation promoting mRNAs Nat Commun 2019-09-13 [PMID: 31519929] (IP, WB, KD, Human)
• Kaehler C, Isensee J, Hucho T et al. 5-Fluorouracil affects assembly of stress granules based on RNA incorporation. Nucleic Acids Res. 2014-04-11 [PMID: 24728989] (ICC/IF, Human)
• Kaehler C, Isensee J, Nonhoff U et al. Ataxin-2-like is a regulator of stress granules and processing bodies. PLoS One. 2012-11-27 [PMID: 23209657] (ICC/IF, Human)
• Thomas MG, Luchelli L, Pascual M et al. A Monoclonal Antibody against p53 Cross-Reacts with Processing Bodies. PLoS One. 2012-05-10 [PMID: 22590546]
• Akhrymuk I, Kulemzin SV, Frolova EI et al. Evasion of the innate immune response: the Old World alphavirus nsP2 protein induces rapid degradation of Rpb1, a catalytic subunit of RNA polymerase II. J Virol 2012-07-01 [PMID: 22514352]
• Huang L, Mollet S, Souquere S et al. Mitochondria associate with P-bodies and modulate microRNA-mediated RNA interference. J Biol Chem 2011-07-01 [PMID: 21576251]
• Chien HL, Liao CL, Lin YL. et al. The FUSE binding protein 1 interacts with untranslated regions of Japanese encephalitis virus RNA and negatively regulates viral replication. J Virol 2011-03-02 [PMID: 21367899]
• Bennasser Y, Chable-Bessia C, Triboulet R et al. Competition for XPO5 binding between Dicer mRNA, pre-miRNA and viral RNA regulates human Dicer levels. Nat Struct Mol Biol 2011-03-01 [PMID: 21297638]
• Taddeo et al. PNAS 106 (29):12139-12144. 2009-01-01 [PMID: 19584246]
• Minshall et al. Mol.Biol.Cell 20 (9):2464-2472. 2009-01-01 [PMID: 19297524]
• Zhai et al. Mol.Cell.Biol. 28 (24):7414-7426. 2008-01-01 [PMID: 18936174]
• Ernoult-Lange M, Wilczynska A, Harper M et al. Nucleocytoplasmic traffic of CPEB1 and accumulation in Crm1 nucleolar bodies. Mol Biol Cell. 2009-01-01 [PMID: 18923137]
• Zheng et al. J.Cell Biol. 182 (1):89-101. 2008-01-01 [PMID: 18625844]
• Morris AR, Mukherjee N, Keene JD et al. Ribonomic analysis of human Pum1 reveals cis-trans conservation across species despite evolution of diverse mRNA target sets. Mol Cell Biol 2008-06-01 [PMID: 18411299]
• Marin et al. J.Virol. 82 (2):987-998. 2008-01-01 [PMID: 17977970]
• Song MG, Kiledjian M. 3' Terminal oligo U-tract-mediated stimulation of decapping RNA. 2007-12-01 [PMID: 17942740]
• Sivan et al. Mol.Cell.Biol. 27 (19):6639-6646. 2007-01-01 [PMID: 17664278]

Bioinformatics

• Gene Symbol: DDX6
• Entrez:
  • 1656(Human)
• Uniprot:
  • P26196(Human)