Dicer Antibody - BSA Free

Western Blot: Dicer Antibody [NBP1-06520] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiDicer antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation: Dicer Antibody [NBP1-06520] - Detection of human Dicer by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Dicer antibody NBP1-06520 (lot NBP1-06520-5) used for IP at 6 ug per reaction. Dicer was also immunoprecipitated by a previous lot of this antibody (lot NBP1-06520-4). For blotting immunoprecipitated Dicer, NBP1-06520 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Western Blot: Dicer Antibody [NBP1-06520] - Nef does not affect siRNA loading into RISC, but inhibits its slicing activity.(A) Lysates from the U937 stable cell lines were prepared in Buffer D as described in Materials & Methods. From this, 25 µg of lysate was incubated with 32P-labeled duplex siRNA (without or with 50-fold molar excess of unlabeled siRNA) in binding buffer for 30 min at 4°C. The complexes were separated on a 6% non-denaturing polyacrylamide gel. (B) The gel shift assay was set up as in (A) except that lysates immunodepleted for either Ago2 or Dicer were also included. The positions of the mobility shifted complexes & free probe are indicated. (C) Slicer activity was assayed using immunoprecipitated Ago2 from U937/Nef-EYFP or U937/EYFP cell lysates & a 32P-labeled in vitro transcribed let7a RNA, as described in Materials & Methods. The positions of full-length RNA & the two sliced products are shown. The 59 nt marker oligonucleotide was run on the same gel, but a lower exposure is shown. (D) Densitometric analysis of autoradiograms from three independent experiments was carried out using Image J (version 1.4.1). The slicing activity in Nef-EYFP lysates is represented as a percentage of that in EYFP lysates. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0074472), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu
• Applications: WB, IP, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Dicer Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 600 and 650 of human double-stranded RNA-specific endoribonuclease; dicer1 using the numbering given in entry NP_085124.2 (GeneID 23405).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DICER1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2- 10 ug/mg lysate
  • Knockout Validated Reported in (PMID: 31703292)
  • Western Blot 1:2000-1:10000
• Theoretical MW: 219 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Appendix Whole Tissue Lysate (Adult Whole Liver Cirrhosis)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Dicer Antibody - BSA Free

• DCR1
• dicer 1, double-stranded RNA-specific endoribonuclease
• dicer 1, ribonuclease type III
• Dicer
• Dicer1, Dcr-1 homolog (Drosophila)
• EC 3.1.26
• EC 3.1.26.-
• EC 3.1.26.3
• Helicase MOI
• Helicase with RNase motif
• helicase-moi
• HERNADicer1, Dcr-1 homolog
• K12H4.8-LIKE
• KIAA0928endoribonuclease Dicer

Background

This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Dicer Antibody (NBP1-06520)(6)

Publications using NBP1-06520

• Thomas KT, Vermare A, Egleston SO et al. MicroRNA 3' ends shorten during adolescent brain maturation Frontiers in molecular neuroscience 2023-04-14 [PMID: 37122627] (WB, Mouse)
• Su X, Jin Y, Shen Y et al. RNAase III-Type Enzyme Dicer Regulates Mitochondrial Fatty Acid Oxidative Metabolism in Cardiac Mesenchymal Stem Cells Int J Mol Sci 2019-11-07 [PMID: 31703292] (WB, KO, Mouse, Human)
• Simeoli R, Montague K, Jones HR et al. Exosomal cargo including microRNA regulates sensory neuron to macrophage communication after nerve trauma Nat Commun. 2017-11-24 [PMID: 29176651] (IP, Mouse)
• Aqil M, Naqvi AR, Bano AS, Jameel S. The HIV-1 Nef Protein Binds Argonaute-2 and Functions as a Viral Suppressor of RNA Interference. PLoS One. 2013-09-04 [PMID: 24023945] (Human)
• Gurtan AM, Lu V, Bhutkar A et al. In vivo structure-function analysis of human Dicer reveals directional processing of precursor miRNAs. RNA 2012-06-01 [PMID: 22546613]
• Tan GS, Garchow BG, Liu X et al. Clarifying mammalian RISC assembly in vitro. BMC Mol Biol;12(1):19. 2011-04-29 [PMID: 21529364]

Review for Dicer Antibody (NBP1-06520) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer WB Mouse 09/13/2011

Summary

• Application: Western Blot
• Sample Tested: NBP1-06520, Sample Amount: 16ug
• Species: Mouse

Bioinformatics

• Gene Symbol: DICER1
• Entrez:
  • 23405(Human)
  • 192119(Mouse)
• Uniprot:
  • Q9UPY3(Human)