DNA Polymerase gamma Antibody - BSA Free

Western Blot: DNA Polymerase gamma Antibody [NBP1-52300] - Analysis of anti-POLG antibody with (30 ug of whole cell lysate). A: 293T. 5% SDS PAGE. POLG antibody diluted at 1:5000.

Immunocytochemistry/Immunofluorescence: DNA Polymerase gamma Antibody [NBP1-52300] - Analysis of anti-POLG antibody with methanol-fixed HeLa cell at 1:200 dilution.

Immunohistochemistry-Paraffin: DNA Polymerase gamma Antibody [NBP1-52300] - Analysis of anti-POLG antibody with human skeletal muscle at concentration 10 ug/ml.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.67 mg/ml

DNA Polymerase gamma Antibody - BSA Free Summary

• Description: Product can be stored undiluted at 4C for up to 1-2 weeks.
• Immunogen: Recombinant protein fragment containing a sequence corresponding to a region within amino acids 829 and 1201 of DNA Polymerase gamma.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POLG
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 1:100-1:200
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:200
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 1:1000-1:10000
• Application Notes: .
• Theoretical MW: 139.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:35121402).

Packaging, Storage & Formulations

• Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Concentration: 0.67 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DNA Polymerase gamma Antibody - BSA Free

• DNA Polymerase gamma
• DNA polymerase subunit gamma-1
• EC 2.7.7.7
• FLJ27114
• MDP1
• MIRAS
• Mitochondrial DNA polymerase catalytic subunit
• MTDPS4A
• POLG
• PolG, catalytic subunit
• POLG1
• POLG1MTDPS4B
• POLGA
• PolG-alpha
• POLGAPEO
• polymerase (DNA directed), gamma
• SANDO
• SCAE

Background

As the only DNA polymerase (pol) present in mitochondria, DNA polymerase gamma (POLG) is necessarily implicated in base excision repair processes to correct oxidative damage to the mitochondrial genome. Therefore, the ability of the catalytic subunit of human POLG has been tested to participate in uracil-provoked base excision repair reconstituted in vitro with purified components. Subsequent to actions of uracil-DNA glycosylase and apurinic/apyrimidinic endonuclease, human POLG is able to fill a single nucleotide gap in the presence of a 5' terminal deoxyribose phosphate (dRP) flap. The catalytic subunit of human pol gamma catalyzes release of the dRP residue from incised apurinic/apyrimidinic sites to produce a substrate for DNA ligase. Faulty replication of the human mitochondrial genome is thought to be the cause of many diseases. The low selectivity of the mitochondrial DNA polymerase is implicated as the cause of many side effects observed in the treatment of viral infections such as HIV.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for DNA Polymerase gamma Antibody (NBP1-52300)(6)

Publications using NBP1-52300

• Latino D, Chieffi Baccari G, Di Fiore MM et al. Autophagy and mitochondrial damage in the testis of high-fat diet fed rats General and comparative endocrinology 2022-08-13 [PMID: 35973585] (WB, Rat)
  
  Details:
  Dilution used for WB 1:1500
• Shin J, Hong S, Choi S et al. Flow-induced endothelial mitochondrial remodeling mitigates mitochondrial reactive oxygen species production and promotes mitochondrial DNA integrity in a p53-dependent manner Redox Biology 2022-04-01 [PMID: 35121402] (WB, Mouse)
• Phillips AF, Millet AR, Tigano M et al. Single-Molecule Analysis of mtDNA Replication Uncovers the Basis of the Common Deletion Mol. Cell 2017-01-11 [PMID: 28111015]
• Li S, Guo J, Ying Z et al. Valproic acid-induced hepatotoxicity in alpers syndrome is associated with mPTP opening dependent apoptotic sensitivity in an iPSC model Hepatology. 2015-01-20 [PMID: 25605636] (WB, Human)
• Roos S, Macao B, Fuste JM et al. Subnormal levels of POLgammaA cause inefficient initiation of light-strand DNA synthesis and lead to mitochondrial DNA deletions and autosomal dominant progressive external ophthalmoplegia. Hum Mol Genet 2013-03-06 [PMID: 23446635] (WB, Human)
• Wu Y, Li N, Zhang T et al. Mitochondrial DNA base excision repair and mitochondrial DNA mutation in human hepatic HuH-7 cells exposed to stavudine. Mutat Res 2009-05-12 [PMID: 19428378] (WB, Human)

FAQs for DNA Polymerase gamma Antibody (NBP1-52300). (Showing 1 - 1 of 1 FAQs).

1. I am planning on using your antibody NBP1-52300 and am wondering about the immunogen. I understand that you might not be able to give me the exact sequence, but it is of great importance to me that the antibody does not bind to amino acids 994-1040. Is it possible for you to answer whether the antibody binds to this position or not?
   • The antibody was raised against recombinant protein fragment containing a sequence corresponding to a region within amino acids 829 and 1201 of DNA polymerase gamma. We typically do not epitope map our antibodies and I cannot determine if this antibody does or does not bind to amino acids 994-1040 however the immunogen sequence does include that fragment.

Bioinformatics

• Gene Symbol: POLG
• Entrez:
  • 5428(Human)
• Uniprot:
  • P54098(Human)