Dopamine beta-Hydroxylase Antibody

Western Blot: Dopamine beta-Hydroxylase Antibody [NBP1-31386] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Dopamine beta hydroxylase antibody and a competitor's antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP1-31386] - RT2 xenograft. Dopamine beta hydroxylase antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP1-31386] - U87 xenograft. Dopamine beta hydroxylase antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP1-31386] - Mouse brain. Dopamine beta hydroxylase antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Dopamine beta-Hydroxylase Antibody [NBP1-31386] - Mouse brain. Dopamine beta Hydroxylase stained by Dopamine beta Hydroxylase antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: Dopamine beta-Hydroxylase Antibody [NBP1-31386] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dopamine beta Hydroxylase antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Dopamine beta-Hydroxylase Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Dopamine beta-Hydroxylase. The exact sequence is proprietary.
• Localization: Soluble dopamine beta-hydroxylase: Cytoplasmic vesicle, secretory vesicle lumen, Cytoplasmic vesicle, secretory vesicle, chromaffin granule lumen, secretory vesicle membrane, chromaffin granule membrane
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DBH
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 69 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rhesus Macaque (86%), Bovine (80%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Dopamine beta-Hydroxylase Antibody

• DBH
• DBM
• dopamine beta-hydroxylase (dopamine beta-monooxygenase)
• Dopamine betaHydroxylase
• Dopamine beta-Hydroxylase
• Dopamine beta-monooxygenase
• DOPBHY
• EC 1.14.17.1

Background

The production of neurotransmitters is controlled by environmental stimuli, which send signals through second messengers and protein kinase pathways. Regulation of gene expression of dopamine beta hydroxylase and tyrosine hydroxylase, two of the catecholamine biosynthetic enzymes, by cyclic AMP, diacyl glycerol, and Ca2+ leads to increased neurotransmitter biosynthesis. Dopamine beta-hydroxylase catalyzes the conversion of dopamine to noradrenaline and is expressed selectively in noradrenergic and adrenergic neurons in the nervous system. The disruption of the DBH gene is thought to cause changes in development and behavior in certain models. Both DBH and the multiple regulatory pathways of DBH are of increasing research in many neurological diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Dopamine beta-Hydroxylase Antibody (NBP1-31386)(5)

Publications using NBP1-31386

• Valsecchi V, Errico F, Bassareo V et al. SMN deficiency perturbs monoamine neurotransmitter metabolism in spinal muscular atrophy Communications biology 2023-11-13 [PMID: 37957344] (WB, Mouse)
  
  Details:
  1:1000 WB dilution
• van der Keylen P, Garreis F, Steigleder R et al. Involvement of catecholaminergic neurons in motor innervation of striated muscle in the mouse esophagus. Histochem. Cell Biol. 2016-01-21 [PMID: 26794326]
• Scifo E, Szwajda A, Soliymani R et al. Quantitative analysis of PPT1 interactome in human neuroblastoma cells. Data Brief 2015-09-01 [PMID: 26217791] (Human)
• Scifo E, Szwajda A, Soliymani R et al. Proteomic analysis of the palmitoyl protein thioesterase 1 interactome in SH-SY5Y human neuroblastoma cells J Proteomics 2015-04-10 [PMID: 25865307] (WB, Human)
• Hilton JBW, Kysenius K, Liddell JR, Rautengarten C Disrupted copper availability in sporadic ALS: Implications for CuII (atsm) as a treatment option BioRxiv 2020-01-01 (WB, Mouse, Human)

Bioinformatics

• Gene Symbol: DBH
• Entrez:
  • 1621(Human)
• Uniprot:
  • P09172()