Ferritin Heavy Chain Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Ferritin Heavy Chain Antibody [NBP1-31944] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ferritin Heavy Chain protein stained by Ferritin Heavy Chain antibody [N1C3] diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:50. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Western Blot: Ferritin Heavy Chain Antibody [NBP1-31944] - Ferritin Antibody [NBP1-31944] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] diluted at 1:500.

Western Blot: Ferritin Heavy Chain Antibody [NBP1-31944] - Human tissue extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] diluted at 1:500.

Immunohistochemistry-Paraffin: Ferritin Heavy Chain Antibody [NBP1-31944] - Rat liver. Ferritin Heavy Chain antibody [N1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: Ferritin Heavy Chain Antibody [NBP1-31944] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: Ferritin Heavy Chain Antibody [NBP1-31944] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Ferritin Heavy Chain Antibody [NBP1-31944] - Ferritin Heavy Chain antibody [N1C3] detects Ferritin Heavy Chain protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.Ferritin Heavy Chain stained by Ferritin Heavy Chain antibody [N1C3] (NBP1-31944) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: Ferritin Heavy Chain Antibody [NBP1-31944] - Ferritin Heavy Chain antibody [N1C3] detects Ferritin Heavy Chain protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.Ferritin Heavy Chain stained by Ferritin Heavy Chain antibody [N1C3] (NBP1-31944) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: Ferritin Heavy Chain Antibody [NBP1-31944] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

• Reactivity: Hu, Mu, Rt, Bv, Ca, Ch, Fe, Gp, RM, Sh
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Ferritin Heavy Chain Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Ferritin Heavy Chain. The exact sequence is proprietary.
• Predicted Species: Sheep (93%), Chicken (93%), Canine (96%), Bovine (92%), Guinea Pig (96%), Feline (96%), Rhesus Macaque (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FTH1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Porcine (87%), Cat (96%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Ferritin Heavy Chain Antibody - BSA Free

• apoferritin
• Cell proliferation-inducing gene 15 protein
• Ferritin H subunit
• ferritin heavy chain
• ferritin, heavy polypeptide 1
• FHC
• FTHEC 1.16.3.1
• FTHL6MGC104426
• PIG15
• placenta immunoregulatory factor
• PLIF
• proliferation-inducing protein 15

Background

This gene encodes the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases. This gene has multiple pseudogenes. Several alternatively spliced transcript variants have been observed, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ferritin Heavy Chain Antibody (NBP1-31944)(8)

Publications using NBP1-31944

• Chen Z, Wang F, Chen Z, Zheng N et Al. Decursin ameliorates neurotoxicity induced by glutamate through restraining ferroptosis by up-regulating FTH1 in SH-SY5Y neuroblastoma cells Neuroscience 2024-08-28 [PMID: 39197742]
• Yang WP, Li MQ, Ding J et Al. High-altitude hypoxia exposure inhibits erythrophagocytosis by inducing macrophage ferroptosis in the spleen Elife 2024-04-17 [PMID: 38629942]
• Khang Nguyen, Jialing Tang, Sungji Cho, Fan Ying, Hye Kyoung Sung, James Wonsuk Jahng, Kostas Pantopoulos, Gary Sweeney Salubrinal promotes phospho-eIF2 alpha -dependent activation of UPR leading to autophagy-mediated attenuation of iron-induced insulin resistance Molecular Metabolism 2024-03-26 [PMID: 38527647]
• Liu D, Wang T, Wang Q et al. Identification of key genes in sepsis-induced cardiomyopathy based on integrated bioinformatical analysis and experiments in vitro and in vivo PeerJ 2023-11-21 [PMID: 38025678]
• Mi Y, Wei C, Sun L et al. Melatonin inhibits ferroptosis and delays age-related cataract by regulating SIRT6/p-Nrf2/GPX4 and SIRT6/NCOA4/FTH1 pathways Biomedicine & Pharmacotherapy 2023-01-01 [PMID: 36463827] (WB, Rat)
• Lee YJ, Kim GH, Yoo JS et al. Vitamin D rescues pancreatic beta cell dysfunction due to iron overload via elevation of the vitamin D receptor and maintenance of Ca2+ homeostasis Molecular nutrition & food research 2020-12-15 [PMID: 33325123]
• Zhang Q, Wang J, Huang F et al. Leptin induces NAFLD progression through infiltrated CD8+ T lymphocytes mediating pyroptotic-like cell death of hepatocytes and macrophages Dig Liver Dis 2020-11-07 [PMID: 33172809] (WB, Rat)
• James Won Suk Jahng, Reham Musaibeh Alsaadi, Rengasamy Palanivel, Erfei Song, Victoria Emily Barbosa Hipolito, Hye Kyoung Sung, Roberto Jorge Botelho, Ryan Charles Russell, Gary Sweeney Iron overload inhibits late stage autophagic flux leading to insulin resistance EMBO Reports 2019-08-23 [PMID: 31441223]

Bioinformatics

• Gene Symbol: FTH1