FosB/G0S3 Antibody (83B1138) - Azide and BSA Free

Western Blot: FosB/G0S3 Antibody (83B1138) - Azide Free [NBP2-33219] - Analysis of FosB in HeLa cell lysate where bands are observed at ~38 kDa representing FosB and at ~35 kDa representing delta FosB. Image from the standard format of this antibody.

• Reactivity: Hu
• Applications: WB
• Clone: 83B1138
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 0.5 mg/ml

FosB/G0S3 Antibody (83B1138) - Azide and BSA Free Summary

• Immunogen: This antibody was generated by immunizing mice with a synthetic peptide corresponding to amino acids 2-15 of human FosB.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FOSB
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/ml
• Application Notes: In HeLa, a 46 kDa band is observed.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for FosB/G0S3 Antibody (83B1138) - Azide and BSA Free

• activator protein 1
• AP-1
• DKFZp686C0818
• FBJ murine osteosarcoma viral oncogene homolog B
• FosB
• G0S3
• G0S3G0/G1 switch regulatory protein 3
• GOS3
• GOSB
• MGC42291
• oncogene FOS-B
• protein fosB

Background

Fos and Jun dimerize to form Activator Protein-1 (AP-1), a transcriptional factor that binds to the 12-O-tetradecanoylphorbol 13-acetate (TPA) response element (TRE) of several cellular and viral genes including human collagenase, metallothionein IIa, stromelysin, interleukin 2, SV40 and polyoma. Fos and Jun contain the 'leucine-zipper' motif that allows for dimerization and an adjacent basic domain required for biological activity. The functionally active form of Fos is in a heterodimer with a member of the Jun family. While Jun family members can form functional homodimers, studies indicate that Fos family members do not self-associate and therefore do not bind DNA on their own. The various dimers differ in their ability to transactivate AP-1 dependent genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FOSB