FSHR Antibody

Western Blot: FSHR Antibody [NBP2-16537] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FSH Receptor antibody [C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: FSH R Antibody [NBP2-16537] - Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FSH Receptor protein stained by FSH Receptor antibody C-term diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Western Blot: FSHR Antibody [NBP2-16537] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FSH Receptor antibody [C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Product Discontinued

FSHR Antibody Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FSHR. The exact sequence is proprietary.
• Localization: Cell membrane; Multi-pass membrane protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FSHR
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 78 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for FSHR Antibody

• follicle stimulating hormone receptor
• follicle-stimulating hormone receptor
• Follitropin receptor
• FSH R
• FSH receptor
• FSHR
• FSH-R
• LGR1
• LGR1FSHRO
• MGC141667
• MGC141668
• ODG1

Background

The protein encoded by this gene belongs to family 1 of G-protein coupled receptors. It is the receptor for follicle stimulating hormone and functions in gonad development. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for FSHR Antibody (NBP2-16537)(1)

Publication using NBP2-16537

• Li X, Zhu Q, Wang W, et al. Elevated chemerin induces insulin resistance in human granulosa-lutein cells from polycystic ovary syndrome patients. FASEB J 2019-07-18 [PMID: 31311314] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: FSHR
• Uniprot:
  • P23945(Human)