GABA-A R beta 2 Antibody - Azide Free

Western Blot: GABA-A R beta 2 Antibody [NB300-198] - 7 ug of rat cerebellum (Cb) showing specific immunolabeling of the ~55k beta2-subunit of the GABA-AR.

• Reactivity: Mu, Rt, Hu, Bv, Ca, Pm
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

GABA-A R beta 2 Antibody - Azide Free Summary

• Immunogen: Fusion protein from the cytoplasmic loop of the beta 2 subunit. Accession # P63138
• Localization: Cell Membrane; multi pass membrane protein
• Specificity: Specific for endogenous levels of the ~55 kDa beta 2-subunit of the GABAA receptor.
• Predicted Species: Human (100%), Canine (100%), Primate (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GABRB2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Application Notes: Immunoprecipitation 15 ug ** ** 68% of all GABA-A receptors in dodecylsulfate extracts of rat forebrains (150 ul)
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 16807363).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for GABA-A R beta 2 Antibody - Azide Free

• GABA A R beta 2
• GABA(A) receptor subunit beta-2
• GABAAR beta 2
• GABAARb2
• GABRB2
• gamma-aminobutyric acid (GABA) A receptor, beta 2
• gamma-aminobutyric acid A receptor beta 2
• gamma-aminobutyric acid receptor subunit beta-2
• MGC119386
• MGC119388
• MGC119389

Background

GABA (A) receptors are ligand-gated chloride channels that play a role as inhibitory neurotransmitters. They are known targets for certain classes of environmental and pharmaceutical compounds because a number of drugs interact with binding sites on GABA (A). Some of these drugs include benzodiazepines, anticonvulsants, and anesthetics. GABA (A) Receptors are comprised of subunits and these are further classified into 3 major groups (alpha, beta, and gamma). The subunits determine the pharmacological characteristics.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GABA-A R beta 2 Antibody (NB300-198)(4)

Publications using NB300-198

• Bohnsack JP, Carlson SL, Morrow AL. Differential regulation of synaptic and extrasynaptic alpha4 GABA(A) receptor populations by protein kinase A and protein kinase C in cultured cortical neurons. Neuropharmacology 2016-06-01 [PMID: 26767953]
• Werner DF. Elucidating the Role of Alpha1-containing GABA(A) Receptors in Ethanol Action PhD Thesis University of Pittsburgh (ISBN 0549455426, 9780549455424). 2007-01-01 (WB, Mouse)
• Iba H, Watanabe T, Motomura S et al. A Japanese herbal medicine attenuates anxiety-like behavior through GABAA receptor and brain-derived neurotrophic factor expression in a rat model of premenstrual syndrome Journal of pharmacological sciences 2021-01-01 [PMID: 33357772] (WB, Rat)
• Borghese CM, Werner DF, Topf N et al. An isoflurane- and alcohol-insensitive mutant GABA(A) receptor alpha(1) subunit with near-normal apparent affinity for GABA: characterization in heterologous systems and production of knockin mice. J Pharmacol Exp Ther. 2006-10-01 [PMID: 16807363] (WB, Mouse)

FAQs for GABA-A R beta 2 Antibody (NB300-198). (Showing 1 - 1 of 1 FAQs).

1. Could you please provide the immunogen sequence for Anti-GABA A Receptor beta 3 antibody and Anti-GABA A Receptor beta 2 antibody, catalog number NB300-199 and NB300-198 at your earliest convenience? If the exact sequence is not available, could you please tell me a broader range of amino acids or the region of the peptide from which it is derived? Also, please let me know if this information can be made public. Thank you for your help in this matter.
   • The immunogen for NB300-199 falls within 340-420 amino acids, while the immunogen for NB300-198 falls within the range of 327-451.

Bioinformatics

• Gene Symbol: GABRB2
• Entrez:
  • 2561(Human)
  • 25451(Rat)
• Uniprot:
  • P63138(Rat)