GRHL2 Antibody (CL3760) - BSA Free

Immunocytochemistry/Immunofluorescence: GRHL2 Antibody (CL3760) [NBP2-61634] - Staining of A-431 cells using the Anti-GRHL2 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Immunohistochemistry-Paraffin: GRHL2 Antibody (CL3760) [NBP2-61634] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: GRHL2 Antibody (CL3760) [NBP2-61634] - Staining of human rectum shows strong nuclear immunoreactivity in glandular cells.

Immunohistochemistry-Paraffin: GRHL2 Antibody (CL3760) [NBP2-61634] - Staining of human kidney shows nuclear positivity in a subset of renal tubules.

Immunohistochemistry-Paraffin: GRHL2 Antibody (CL3760) [NBP2-61634] - Staining of human liver shows absence of immunoreactivity (negative control).

Immunohistochemistry-Paraffin: GRHL2 Antibody (CL3760) [NBP2-61634] - Staining of human prostate shows strong nuclear immunoreactivity in glandular cells.

Immunohistochemistry-Paraffin: GRHL2 Antibody (CL3760) [NBP2-61634] - Staining of human skin shows strong nuclear positivity in epithelial cells.

Immunohistochemistry-Paraffin: GRHL2 Antibody (CL3760) [NBP2-61634] - Staining of human fallopian tube shows strong nuclear immunoreactivity in glandular cells.

Immunohistochemistry-Paraffin: GRHL2 Antibody (CL3760) [NBP2-61634] - Staining of human endometrium tube shows nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: GRHL2 Antibody (CL3760) [NBP2-61634] - Analysis in human prostate and liver tissues using NBP2-61634 antibody. Corresponding GRHL2 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: GRHL2 Antibody (CL3760) [NBP2-61634] - Staining of human prostate shows strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: GRHL2 Antibody (CL3760) [NBP2-61634] - Staining of human skin shows strong nuclear positivity in squamous epithelial cells.

Immunohistochemistry-Paraffin: GRHL2 Antibody (CL3760) [NBP2-61634] - Staining of human liver shows no positivity in hepatocytes as expected.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clone: CL3760
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

GRHL2 Antibody (CL3760) - BSA Free Summary

• Immunogen: This antibody was developed using a recombinant protein derived from Q6ISB3, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GRHL2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:5000 - 1:10000
  • Immunohistochemistry-Paraffin 1:5000 - 1:10000
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
• Control Peptide:

  GRHL2 Recombinant Protein Antigen (NBP2-61634PEP)

Reactivity Notes

Expected to cross react based on sequence identity: Mouse (87%), Rat (87%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for GRHL2 Antibody (CL3760) - BSA Free

• BOMMGC149294
• Brother of mammalian grainyhead
• deafness, autosomal dominant 28
• DFNA28
• FLJ13782
• grainyhead-like 2 (Drosophila)
• grainyhead-like protein 2 homolog
• TFCP2L3MGC149295
• Transcription factor CP2-like 3FLJ11172

Background

TFCP2L3 is a member of a family of transcription factor genes whose archetype is TFCP2 (MIM 189889), a mammalian ortholog of the Drosophila gene 'grainyhead' (grh).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GRHL2