HMGB1/HMG-1 Antibody Pair

Sandwich ELISA: HMGB1 Antibody Pair [H00003146-AP44] - [H00003146-P01] - Standard curve using recombinant protein as an analyte.

Sandwich ELISA: HMGB1 Antibody Pair [H00003146-AP44] - Detection sensitivity ranging from 3 ng/ml to 100 ng/ml.

• Reactivity: Hu
• Applications: ELISA
• Standard Curve Range: 3 ng/ml to 100 ng/ml

HMGB1/HMG-1 Antibody Pair Summary

• Description: Quality control test: Standard curve using recombinant protein (H00003146-P01) as an analyte. This antibody pair set comes with matched antibody pair to detect and quantify protein level of human HMGB1.
• Standard Curve Range: 3 ng/ml to 100 ng/ml
• Assay Type: Sandwich ELISA
• Gene: HMGB1

Applications/Dilutions

• Dilutions:
  • Sandwich ELISA 3 ng/ml - 100 ng/ml
• Application Notes: Reagents are sufficient for at least 3-5 x 96 well plates using recommended protocols.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 1x PBS, pH 7.4

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for HMGB1/HMG-1 Antibody Pair

• Amphoterin
• high mobility group box 1
• High mobility group protein 1
• high mobility group protein B1
• high-mobility group (nonhistone chromosomal) protein 1
• high-mobility group box 1
• HMG1
• HMG-1
• HMG1DKFZp686A04236
• HMG3
• HMGB1
• SBP-1
• Sulfoglucuronyl carbohydrate binding protein

Background

HMGB1 and HMGB2 are part of the chromatin non-histone high mobility group proteins 1 and 2. HMGB1 and HMGB2 (containing multiple HMG-boxes) are conserved domains of 80 amino acids which mediate the DNA binding of many proteins. HMG box domains recognize DNA structure. Both HMGB1 and HMGB2 contain a N-terminal HMG box, a central HMG box, and an acidic carboxy terminus. The acidic tails of HMGB1 and HMGB2 contain multiple serine residues which match the phosphorylation consensus sites of casein kinase II, and phosphorylation of this domain appears to be important for proper functioning of these proteins. HMGB1 and HMGB2 have been shown to facilitate the binding of various sequence-specific transcription factors to their respective DNA binding sites. HMGB1 and HMGB2 may also serve as architectural factors that recognize and mediate DNA structural changes that accompany various events such as DNA repair, transcription, and replication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: HMGB1