Rat HMGB1/HMG-1 ELISA Kit (Colorimetric)

ELISA: Rat HMGB1/HMG-1 ELISA Kit (Colorimetric) [NBP3-06661] - Samples were spiked with high concentrations of Rat HMGB1/HMG-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat HMGB1/HMG-1 ELISA Kit (Colorimetric) [NBP3-06661] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 31.25 - 2000 pg/mL
• Sensitivity: 18.75 pg/mL

Rat HMGB1/HMG-1 ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 31.25 - 2000 pg/mL
• Sensitivity: 18.75 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 5
• Intra-Assay: %CV < 5
• Spike Recovery: average = 94% for serum; 98% for EDTA plasma; 97% for Cell culture media
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: HMGB1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat HMGB1/HMG-1 ELISA Kit (Colorimetric)

• Amphoterin
• high mobility group box 1
• High mobility group protein 1
• high mobility group protein B1
• high-mobility group (nonhistone chromosomal) protein 1
• high-mobility group box 1
• HMG1
• HMG-1
• HMG1DKFZp686A04236
• HMG3
• HMGB1
• SBP-1
• Sulfoglucuronyl carbohydrate binding protein

Background

HMGB1 and HMGB2 are part of the chromatin non-histone high mobility group proteins 1 and 2. HMGB1 and HMGB2 (containing multiple HMG-boxes) are conserved domains of 80 amino acids which mediate the DNA binding of many proteins. HMG box domains recognize DNA structure. Both HMGB1 and HMGB2 contain a N-terminal HMG box, a central HMG box, and an acidic carboxy terminus. The acidic tails of HMGB1 and HMGB2 contain multiple serine residues which match the phosphorylation consensus sites of casein kinase II, and phosphorylation of this domain appears to be important for proper functioning of these proteins. HMGB1 and HMGB2 have been shown to facilitate the binding of various sequence-specific transcription factors to their respective DNA binding sites. HMGB1 and HMGB2 may also serve as architectural factors that recognize and mediate DNA structural changes that accompany various events such as DNA repair, transcription, and replication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for HMGB1/HMG-1 ELISA Kit (NBP3-06661)(3)

Publications using NBP3-06661

• Hung CF, Chiu WC, Chen JC et Al. NRICM101 prevents kainic acid-induced seizures in rats by modulating neuroinflammation and the glutamatergic system Int Immunopharmacol 2024-08-01 [PMID: 39094361] (ELISA)
• Chan Ho Kim, Hye-Young Kang, Gyuri Kim, Jimin Park, Bo Young Nam, Jung Tak Park, Seung Hyeok Han, Shin-Wook Kang, Tae-Hyun Yoo Soluble receptors for advanced glycation end-products prevent unilateral ureteral obstruction-induced renal fibrosis Frontiers in Pharmacology 2023-05-16 [PMID: 37261287] (, ELISA)
• Jang SB, Jin SM, Kim HS et al. DAMP-modulating nanoparticle for successful pancreatic islet and stem cell transplantation Biomaterials 2022-07-12 [PMID: 35849998] (ELISA)

Bioinformatics

• Gene Symbol: HMGB1