hnRNP F Antibody - BSA Free

Western Blot: hnRNP F Antibody [NBP1-33607] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP F antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: hnRNP F Antibody [NBP1-33607] - Paraformaldehyde-fixed A431, using hnRNP F (NBP1-33607) antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: hnRNP F Antibody [NBP1-33607] - Mouse uterus. hnRNP F antibody [N1N3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: hnRNP F Antibody [NBP1-33607] - HepG2 whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP F antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: hnRNP F Antibody [NBP1-33607] - A549 Xenograft , using hnRNP F antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunoprecipitation: hnRNP F Antibody [NBP1-33607] - Immunoprecipitation of hnRNP F protein from 293T whole cell extracts using 5 ug of hnRNP F antibody [N1N3]. Western blot analysis was performed using hnRNP F antibody [N1N3]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

• Reactivity: Hu, Mu, Rt, Bv, Pm, RM
• Applications: WB, ICC/IF, IHC, IP, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

hnRNP F Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human hnRNP F. The exact sequence is proprietary.
• Localization: Nucleus, nucleoplasm
• Predicted Species: Rat (98%), Bovine (97%), Chimpanzee (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HNRNPF
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Knockdown Validated Validation (PMID: 31814907).
  • Western Blot 1:500-1:3000
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for hnRNP F Antibody - BSA Free

• heterogeneous nuclear ribonucleoprotein F
• hnRNP F
• HNRPFHnRNP F protein
• mcs94-1
• MGC110997
• Nucleolin-like protein mcs94-1
• OK/SW-cl.23

Background

Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to pre-mRNA processing and transport, and also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II. hnRNP complexes are the major constituents of the spliceosome and in particular, the hnRNP A1 protein is one of the major premRNA/ mRNA binding proteins and also one of the most abundant proteins in the nucleus. hnRNP A1 and A2/B1 regulate the processing of pre-mRNA by directly antagonizing the association of various splicing factors and by influencing the splice site selection on pre-mRNA. The majority of hnRNP proteins components are localized to the nucleus; however some shuttle between the nucleus and the cytoplasm. Most hnRNP proteins, including hnRNP C1 and C2, contain one or more RNA binding domains and are implicated in the processing of pre-mRNA. hnRNPs F and H are largely related factors that preferentially associate with poly(rG) regions on RNA. Isoforms of these proteins are often generated by alternative processing of the premRNA and by posttranslational modifications such as phosphorylation on serines and threonines and methylation of arginines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for hnRNP F Antibody (NBP1-33607)(3)

Publications using NBP1-33607

• Xiong L, Gong Y, Liu H et Al. circGlis3 promotes ?-cell dysfunction by binding to heterogeneous nuclear ribonucleoprotein F and encoding Glis3-348aa protein iScience 2023-12-09 [PMID: 38226164]
• Zhao Q, Liu J, Deng H et al. Targeting Mitochondria-Located circRNA SCAR Alleviates NASH via Reducing mROS Output Cell 2020-09-05 [PMID: 32931733] (WB, Human, Mouse)
• Li F, Su M, Zhao H HnRNP-F promotes cell proliferation by regulating TPX2 in bladder cancer Am J Transl Res 2019-11-15 [PMID: 31814907] (KD, IP, WB, Human)

Bioinformatics

• Gene Symbol: HNRNPF