HOMER3 Antibody - BSA Free

Immunohistochemistry-Paraffin: HOMER3 Antibody [NBP2-32607] - Analysis in human spleen and pancreas tissues. Corresponding HOMER3 RNA-seq data are presented for the same tissues.

Western Blot: HOMER3 Antibody [NBP2-32607] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue

Immunocytochemistry/Immunofluorescence: HOMER3 Antibody [NBP2-32607] - Staining of human cell line A-431 shows localization to plasma membrane & cytosol. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: HOMER3 Antibody [NBP2-32607] - Staining of human spleen shows strong cytoplasmic positivity in red pulp.

Immunohistochemistry-Paraffin: HOMER3 Antibody [NBP2-32607] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.

Immunohistochemistry-Paraffin: HOMER3 Antibody [NBP2-32607] - Staining of human liver shows no positivity in hepatocytes.

Immunohistochemistry-Paraffin: HOMER3 Antibody [NBP2-32607] - Staining of human lymphoid tissues shows no positivity in non-germinal center cells.

Immunohistochemistry-Paraffin: HOMER3 Antibody [NBP2-32607] - Staining of human pancreas shows no positivity in exocrine glandular cells.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HOMER3 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: ALVQTKDQEIQTLKSQTGGPREALEAAEREETQQKVQDLETRNAELEHQLRAMERSLEEARAERERARAEVGRAAQLLDVRLFELSELREGL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HOMER3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  HOMER3 Protein (NBP2-32607PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (88%). Use in Mouse reported in scientific literature (PMID:32070057).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for HOMER3 Antibody - BSA Free

• homer homolog 3 (Drosophila)
• homer protein homolog 3
• Homer, neuronal immediate early gene, 3
• HOMER-3
• VESL3

Background

This gene encodes a member of the homer family of dendritic proteins. Members of this family regulate group 1 metabotrophic glutamate receptor function. The encoded protein may be involved in cell growth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HOMER3 Antibody (NBP2-32607)(1)

Publication using NBP2-32607

• Gritli-Linde A Distinct and Overlapping Expression Patterns of the Homer Family of Scaffolding Proteins and Their Encoding Genes in Developing Murine Cephalic Tissues Int J Mol Sci 2020-02-13 [PMID: 32070057] (IF/IHC, IHC-P, Mouse)

Bioinformatics

• Gene Symbol: HOMER3