HTRA1/PRSS11 Antibody

Western Blot: HTRA1/PRSS11 Antibody [NBP2-23869] - Analysis of HtrA1 in human A) HeLa cell lysate (1 minute exposure) and B) human 293 cell lysate (15 minute exposure) using NBP2-23869 at 1:500.

Simple Western: HTRA1/PRSS11 Antibody [NBP2-23869] - Simple Western lane view shows a specific band for htrA1 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt, Bv, Ca, Pm
• Applications: WB, Simple Western, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

HTRA1/PRSS11 Antibody Summary

• Immunogen: Within the range of amino acids 234-262of human HtrA1 were used as the immunogen, Gen Bank no NP_002766.1. This peptide sequence is in the serine protease domain of the HtrA1 protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HTRA1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Simple Western 1:5000
  • Western Blot 1:250-1: 1000
• Application Notes: In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in Hek293 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:5000, apparent MW was 67 kDa.

Reactivity Notes

This antibody is reactive to Chimpanzee

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.1 ml crude whole Rabbit sera with 50% Glycerol
• Preservative: 0.025% Sodium Azide
• Purity: Unpurified

Alternate Names for HTRA1/PRSS11 Antibody

• ARMD7
• EC 3.4.21
• EC 3.4.21.108
• High-temperature requirement A serine peptidase 1
• HtrA serine peptidase 1
• HTRA1
• IGFBP5-protease
• L56
• ORF480
• PRSS11
• Serine protease 11
• serine, 11 (IGF binding)

Background

htrA1 encodes a member of the trypsin family of serine proteases. This protein is a secreted enzyme that is proposed to regulate the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. It has also been suggested to be a regulator of cell growth. Variations in the promoter region of this gene are the cause of susceptibility to age-related macular degeneration type 7. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HTRA1/PRSS11 Antibody (NBP2-23869)(2)

Publications using NBP2-23869

• Wang N, Eckert KA, Zomorrodi AR et al. Down-regulation of HtrA1 activates the epithelial-mesenchymal transition and ATM DNA damage response pathways. PLoS One. 2012-01-01 [PMID: 22761798]
• Clawson GA, Bui V, Xin P et al. Intracellular localization of the tumor suppressor HtrA1/Prss11 and its association with HPV16 E6 and E7 proteins. J Cell Biochem. 2008-09-01 [PMID: 18452160] (ICC/IF, Human)
  
  Details:
  CaSki human cervical cancer and HPV 16 E6/E7 293T stably transfected cells: 1. IF/ICC (Fig 1). Cytoplasmic and nuclear staining was observed in all CaSki and 293T cell lines examined.

Bioinformatics

• Gene Symbol: HTRA1
• Uniprot:
  • Q92743()