Recombinant Porcine IL-1 RAcP/IL-1 R3 Protein

SDS-Page: Porcine IL-1 RAcP/IL-1 R3 Protein [NBP2-35223]

Product Discontinued

Recombinant Porcine IL-1 RAcP/IL-1 R3 Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 152 amino acids corresponding to IL-1 RAcP/IL-1 R3 Source: E. coli

  Uniprot ID: Q29056

  Amino Acid Sequence: HPLGKRPCRM QAFRIWDVNQ KTFYLRNNQL VAGYLQGPNT KLEEKIDVVP VEPHFVFLGI HGGKLCLSCV KSGDEMKLQL DAVNITDLRK NSEQDKRFTF IRSDSGPTTS FESAACPGWF LCTALEADQP VGLTNTPKAA VKVTKFYFQQ DQ

• Details of Functionality: IL1RAP Protein fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-1alpha-dependent proliferation of murine D10S cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 x 10^4 IU/mg in the presence of 75 pg/ml rPoIL-1alpha.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: IL1RAP
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 0.1 EU/ug of IL-1 RAcP/IL-1 R3 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 17.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 1 x PBS, pH 7.4, 1 mM DTT.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Porcine IL-1 RAcP/IL-1 R3 Protein

• C3orf13IL-1RAcPIL1R3Interleukin-1 receptor 3
• FLJ37788
• IL-1 R3
• IL-1 RAcP
• IL-1 receptor accessory protein
• IL-1R3
• IL-1R-3
• IL1RAcP
• IL-1RAcP
• IL1RAP
• interleukin 1 receptor accessory protein
• interleukin-1 receptor accessory protein beta
• interleukin-1 receptor accessory protein

Background

IL-1RA was initially called the IL-1 inhibitor which is encoded by the IL1RN gene and it is a member of the interleukin 1 cytokine family. IL-1RA is secreted by various types of cells including immune cells, epithelial cells, and adipocytes. IL-RA has functions of inhibiting the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the co-receptor IL1RAP for signaling. IL-1RA is also used in the treatment of rheumatoid arthritis, an autoimmune disease in which IL-1 plays a key role. The porcine IL-1RA is a single non-glycosylated polypeptide chain containing 152 amino acids and it has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. The protein shows 26 % amino acid homology to IL-1beta and 19 % homology to IL-1alpha. It also shares 78 %~80 % a.a. sequence identity with murine, rat and equine IL-1RA

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: IL1RAP
• Uniprot:
  • Q29056()
  • Q29056()
  • Q29056()