IL-34 Antibody - BSA Free

Western Blot: IL-34 Antibody [NBP1-76356] - Analysis of IL-34 in human brain tissue lysate with IL-34 antibody at 0.25 ug/mL in (A) the absence and (B) the presence of blocking peptide.

Western Blot: IL-34 Antibody [NBP1-76356] - Analysis of 125 ng of recombinant human IL-34 with IL-34 antibody at 1 ug/ml.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

IL-34 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 13 amino acid synthetic peptide from near the center of human IL-34. The immunogen is located within amino acids 60 - 110 of IL-34. Amino Acid Squence: TRLRAQVSERELR
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IL34
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 0.25 ug/ml
• Application Notes: Despite its predicted molecular weight, IL-34 will often run at higher molecular weight in SDS-PAGE.
• Theoretical MW: 42-48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  IL-34 Protein (NBP1-76356PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for IL-34 Antibody - BSA Free

• C16orf77
• IL34
• IL-34
• interleukin 34

Background

Interleukin-34 (IL-34) was identified through a functional screening of a library of secreted proteins after transfection of an embryonic kidney cell line with recombinant cDNAs. Flow cytometry and monocyte viability assays demonstrated increased survival and/or proliferation of monocytes induced by IL-34 and specific binding of IL-34 to CD14+ monocytes. IL-34 is a second ligand to the colony stimulating factor (CSF)-1 receptor in addition to CSF1. Like CSF1, IL-34 stimulates phosphorylation of ERK1 and ERK2 in human monocytes and induces differentiation of bone marrow cells into macrophages. Despite its predicted molecular weight, IL-34 will often run at higher molecular weight in SDS-PAGE.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL34
• Uniprot:
  • ACD13474(Human)