IL-6 Antibody - Azide and BSA Free

Western Blot: IL-6 Antibody [NB600-1131] - Western Blot: IL-6 Antibody [HRP] [NBP1-42762] - Lane 1: Human IL-6. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase Human IL-6 secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 20 kDa for Human IL-6. Other band(s): none. Image using the HRP form of this antibody.

Immunohistochemistry: IL-6 Antibody [NB600-1131] - GBM ECs express IL-6. Mouse GBM was induced by orthotopic injection of GL26 glioma cells into wild-type mouse. The brain sections that include normal brains and tumors were stained with anti-CD31, anti-IL-6, and anti-CSF-1 antibodies. Representative immunofluorescence images are shown. Right, enlarged area in normal and tumor tissues. Bar represents 50?um. Zoom-in factor: 4 Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-03050-0) licensed under a CC-BY license.

Western Blot: IL-6 Antibody [NB600-1131] - Protein was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. The blot shows detection of a band 21 kDa in size corresponding to anti-IL6 antibody. Molecular weight markers are also shown (MW). After transfer, the membrane was blocked for 30 minutes with 1% BSA-TBST. Detection occurred using peroxidase conjugated anti-Rabbit IgG secondary antibody diluted 1:40,000 in blocking buffer for 30 min at RT followed by reaction with FemtoMax chemiluminescent substrate.

Western Blot of IL-6 Antibody. Lane 1: Opal Prestained Molecular Weight Marker

Immunocytochemistry/ Immunofluorescence: IL-6 Antibody [NB600-1131] - GBM ECs express IL-6. a Human brain ECs were treated with glioma-CM for 24 h, & cell lysates were subjected to multiplex cytokine array analysis. Left, a representative blot. Right, quantified dot intensity of most significantly changed cytokines. b Human microvascular brain ECs were treated with glioma-CM that were harvested from different human glioma cells. Cell lysates were immunoblotted. c Human microvascular brain ECs & tumor-associated ECs isolated from different GBM patients were subjected to immunoblot analysis. d Mouse GBM was induced by orthotopic injection of GL26 glioma cells into wild-type mouse. The brain sections that include normal brains & tumors were stained with anti-CD31, anti-IL-6, & anti-CSF-1 antibodies. Representative immunofluorescence images are shown. Right, enlarged area in normal & tumor tissues. Bar represents 50 μm. Zoom-in factor: 4 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29422647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, ELISA, ICC/IF, IHC, IP, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

IL-6 Antibody - Azide and BSA Free Summary

• Description: Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: This whole rabbit serum was prepared by repeated immunizations with recombinant human IL-6 produced in E.coli. (Uniprot: P05231)
• Localization: Secreted
• Specificity: Anti-IL-6 antiserum detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-6 production from fibroblasts).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IL6
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000-1:5000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:400-1:800
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:400 - 1:800
  • Immunoprecipitation 1:400-1:800
  • Knockdown Validated
  • Western Blot 1:500-1:2000
• Application Notes: This product has been tested in western blot. This antibody is suitable for use in neutralizations, ELISA, radioimmunoassay, and immunoprecipitation. Reactivity in other immunoassays is unknown. Expect ~23.7kDa. In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule. For immunoblot use the supernatants or lysates of 2 x 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1% (v/v) human serum plus 10ng/mL E. coli LPS. For immunoprecipitation, pre-clearing with a non-specific rabbit IgG is helpful to reduce background. The antiserum is useful for neutralization of human of IL-6 activity in bioassays. For neutralization, incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended. In neutralization experiments in vitro, this antibody does not result in enhanced activity of IL-6. However, because antibodies to IL-6 may act as a soluble receptor in vivo, some antibodies to IL-6 act as carriers and enhance IL-6 activity.
• Control:

  IL-6 Overexpression Lysate

Reactivity Notes

Use in Rat reported in scientific literature (PMID:33651836).

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: No Preservative
• Purity: Unpurified

Alternate Names for IL-6 Antibody - Azide and BSA Free

• B-cell differentiation factor
• B-cell stimulatory factor 2
• BSF2
• BSF-2
• CDF
• CTL differentiation factor
• HSF
• hybridoma growth factor
• IFNB2
• IFN-beta-2
• IL6
• IL-6
• Interferon beta-2
• interleukin 6 (interferon, beta 2)
• interleukin BSF-2
• interleukin-6
• MGI-2A

Background

The antiserum detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-6 production from fibroblasts). In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule (21-28 kDa). The antiserum is also useful for neutralization of human IL-6 activity in bioassays. For neutralization, incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended. In neutralization experiments in vitro, this antibody does not result in enhanced activity of IL-6. However, because antibodies to IL-6 may act as a soluble receptor in vivo, some antibodies to IL-6 act as carriers and enhance IL-6 activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-6 Antibody (NB600-1131)(69)

Publications using NB600-1131

• Zamani A, EmamiAref P, Kubí?ková L, Hašanová K et Al. Paclitaxel triggers molecular and cellular changes in the choroid plexus Front Pain Res (Lausanne) 2024-12-10 [PMID: 39654799]
• Jeong HY, Park JS, Choi JW et Al. GRIM-19-mediated induction of mitochondrial STAT3 alleviates systemic sclerosis by inhibiting fibrosis and Th2/Th17 cells Exp Mol Med 2024-12-06 [PMID: 39643607]
• Moon J, Cho KH, Jhun J et Al. Small heterodimer partner-interacting leucine zipper protein suppresses pain and cartilage destruction in an osteoarthritis model by modulating the AMPK/STAT3 signaling pathway Arthritis Res Ther 2024-11-13 [PMID: 39533324]
• Bai H, Liu X, Lin M et Al. Progressive senescence programs induce intrinsic vulnerability to aging-related female breast cancer Nat Commun 2024-06-17 [PMID: 38886378]
• Zhou, X;Zhu, Y;Gao, D;Li, M;Lin, L;Wang, Z;Du, H;Xu, Y;Liu, J;He, Y;Guo, Y;Wang, S;Qiao, S;Bao, Y;Liu, Y;Zhang, H; Matrilin-3 supports neuroprotection in ischemic stroke by suppressing astrocyte-mediated neuroinflammation Cell reports 2024-03-21 [PMID: 38520693]
• T. Ollewagen, R.M. Benecke, C. Smith High species homology potentiates quantitative inflammation profiling in zebrafish using immunofluorescence Heliyon 2023-12-13 [PMID: 38187273]
• Jeong HY, Park JS, Woo JS et Al. SARS-CoV-2 spike protein accelerates systemic sclerosis by increasing inflammatory cytokines, Th17 cells, and fibrosis J Inflamm (Lond) 2023-12-21 [PMID: 38129904]
• Tran, TT;Lee, G;Huh, YH;Chung, KH;Lee, SY;Park, KH;Kwon, SH;Kook, MS;Chun, JS;Koh, JT;Ryu, JH; Disruption of cholesterol homeostasis triggers periodontal inflammation and alveolar bone loss Experimental & molecular medicine 2023-12-01 [PMID: 38036731]
• Park JS, Yang S, Song D et al. A newly developed PLD1 inhibitor ameliorates rheumatoid arthritis by regulating pathogenic T and B cells and inhibiting osteoclast differentiation Immunology letters 2023-09-16 [PMID: 37722567] (IHC, Mouse)
• Su-Jin Kim, Se Hui Lee, Binh Do Quang, Thanh-Tam Tran, Young-Gwon Kim, Jun Ko, Weon-Young Choi, Sun Young Lee, Je-Hwang Ryu Avenanthramide-C Shows Potential to Alleviate Gingival Inflammation and Alveolar Bone Loss in Experimental Periodontitis Molecules and Cells 2023-10-31 [PMID: 37641936]
• Zhuang P, Wang D, Zhang K et al. Sorafenib promotes hepatocellular carcinoma invasion via interleukin-6/HIF-1?/PFKFB3 Journal of Cancer 2023-06-26 [PMID: 37476196] (WB, Human)
• Lee M, Ryu H, Hwang J et al. Sirt6 Activation Ameliorates Inflammatory Bone Loss in Ligature-Induced Periodontitis in Mice International Journal of Molecular Sciences 2023-06-27 [PMID: 37445896] (WB, IHC-P, Mouse)
• Zhang T, Zhang Y, Li S et al. Gentianella acuta-derived Gen-miR-1 suppresses myocardial fibrosis by targeting HAX1/HMG20A/Smads axis to attenuate inflammation in cardiac fibroblasts Phytomedicine : international journal of phytotherapy and phytopharmacology 2023-06-16 [PMID: 37352750] (WB, Mouse)
• Dib S, Loiola RA, Sevin E et al. TNF? Activates the Liver X Receptor Signaling Pathway and Promotes Cholesterol Efflux from Human Brain Pericytes Independently of ABCA1 International journal of molecular sciences 2023-03-22 [PMID: 36983062] (Western Blot, Human)
• Yu S, Chung S, Ha H et al. Nanotheranostics of Pre Stenotic Vessels By Target Touch On Signaling of Peptide Navigator Advanced Functional Materials 2021-12-08 (IHC-Fr)
• khalil R, Abdo W, El-Tanbouly G et al. Hibiscus Sabdariffa Topical Extract Ointment Inhibits Inflammatory Signals and Exhibits Antibacterial Activity on the Rat Burn Injury: Emerging Approach for New Application in the Superficial Burn Care Research Square 2022-01-27 (IF/IHC, Rat)
• Obi AT, Sharma SB, Elfline MA et al. Experimental venous thrombus resolution is driven by IL-6 mediated monocyte actions Scientific reports 2023-02-24 [PMID: 36828892] (WB, Mouse)
  
  Details:
  Dilution used in IHC-P 1:800
• Sharma K, Kumar S, Prakash R et al. Chebulinic acid alleviates LPS-induced inflammatory bone loss by targeting the crosstalk between reactive oxygen species/NF kappa B signaling in osteoblast cells Free radical biology & medicine 2022-11-21 [PMID: 36423891] (WB, Mouse)
• Zhao RJ, Lee DG, Park CS et al. Acupuncture Inhibits Morphine Induced-Immune Suppress via Antioxidant System Evidence-based complementary and alternative medicine : eCAM 2022-10-22 [PMID: 36317105] (IF/IHC, Rat)
• Franke V, Meyer S, Schulze-Tanzil GG et al. Complement Regulation in Immortalized Fibroblast-like Synoviocytes and Primary Human Endothelial Cells in Response to SARS-CoV-2 Nucleocapsid Protein and Pro-Inflammatory Cytokine TNFa Life (Basel, Switzerland) 2022-09-30 [PMID: 36294967] (ICC/IF, Human)
  
  Details:
  Dilutuion used in ICC 1:50
• Kang E, Yim HE, Nam YJ et al. Exposure to airborne particulate matter induces renal tubular cell injury in vitro: the role of vitamin D signaling and renin-angiotensin system Heliyon 2022-08-01 [PMID: 36033312] (WB, Human)
  
  Details:
  Dilution used for WB 1:1000
• Na HS, Woo JS, Kim JH et al. Coenzyme Q10 encapsulated in micelles ameliorates osteoarthritis by inhibiting inflammatory cell death PloS one 2022-06-24 [PMID: 35749420] (IF/IHC, Rat)
• Park J, Chi L, Kwon HY et al. Decaffeinated green tea extract as a nature-derived antibiotic alternative: An application in antibacterial nano-thin coating on medical implants Food chemistry 2022-02-10 [PMID: 35168041] (IF/IHC)
• Gutziet O, Iluz R, Ben Asher H et al. Maternal N-Acetyl-Cysteine Prevents Neonatal Hypoxia-Induced Brain Injury in a Rat Model International journal of molecular sciences 2021-12-20 [PMID: 34948425] (WB, Rat)
• Ollewagen T, Powrie YSL, Myburgh KH, Smith C Unresolved intramuscular inflammation, not diminished skeletal muscle regenerative capacity, is at the root of rheumatoid cachexia: insights from a rat CIA model Physiological reports 2021-11-01 [PMID: 34806343] (WB, Rat)
• Risbud M, Novais E, Tran V, et al. Long-term treatment with senolytic drugs Dasatinib and Quercetin ameliorates age-dependent intervertebral disc degeneration in mice Nat Commun 2021-09-04 [PMID: 34480023]
• Chow SKH, Cui C, Cheng KYK Et al. Acute Inflammatory Response in Osteoporotic Fracture Healing Augmented with Mechanical Stimulation is Regulated In Vivo through the p38-MAPK Pathway International journal of molecular sciences 2021-08-13 [PMID: 34445423] (IF/IHC, Mouse)
• Koga Y, Sotokawauchi A, Higashimoto Y Et al. DNA-Aptamer Raised against Receptor for Advanced Glycation End Products Improves Survival Rate in Septic Mice Oxidative medicine and cellular longevity 2021-08-07 [PMID: 34413930]
• Singh J, Thachil T, Eapen M Et Al. Immunohistochemical investigation of cytokine expression levels as biomarkers in transrectal ultrasound-guided needle biopsy specimens of prostate adenocarcinoma Molecular and Clinical Oncology 2021-07-16 [PMID: 34405051] (IHC-P)
• Li Q, Qin L, Li J IL-6 signaling pathway contributes to exercise pressor reflex in rats with femoral artery occlusion in association with Kv4 activity in muscle afferent nerves Physiological reports 2021-07-01 [PMID: 34231965] (IF/IHC)
• Betsou A, Lambropoulou M, Georgakopoulou AE et al. The hepatoprotective effect of silibinin after hepatic ischemia/reperfusion in a rat model is confirmed by immunohistochemistry and qRT-PCR The Journal of pharmacy and pharmacology 2021-04-13 [PMID: 33847359]
• Subburaman M, Edderkaoui B Evaluation of CCL21 role in post-knee injury inflammation and early cartilage degeneration PloS one 2021-03-02 [PMID: 33651836] (IHC-P, Rat)
• Kanno SI, Hara A The mRNA expression of Il6 and Pdcd1 are predictive and protective factors for doxorubicin-induced cardiotoxicity Molecular medicine reports 2021-02-01 [PMID: 33300057] (ICC/IF, Rat)
• Shao H, Li Y, Pastar I et al. Notch1 signaling determines the plasticity and function of fibroblasts in diabetic wounds Life Sci Alliance 2020-12-01 [PMID: 33109684]
• Wu J, Dou Y, Ladiges W Adverse neurological effects of short-term sleep deprivation in aging mice are prevented by SS31 peptide Clocks Sleep 2020-10-22 [PMID: 33089207]
• Balakrishnan I, Danis E, Pierce A et al Senescence Induced by BMI1 Inhibition Is a Therapeutic Vulnerability in H3K27M-Mutant DIPG Cell Rep 2020-10-21 [PMID: 33086074] (IF/IHC, WB, IF/IHC, WB, Human)
  
  Details:
  Citation using the IHC-Prediluted version of this antibody.
• El-Zayadi AA, Mohamed SA, Arafa M et al. Anti-IL-6 receptor monoclonal antibody as a new treatment of endometriosis Immunol. Res. 2020-09-17 [PMID: 32939649]
• Vaidya F, Sharma R, Shaikh S et al. Pluronic micelles encapsulated curcumin manifests apoptotic cell death and inhibits pro-inflammatory cytokines in human breast adenocarcinoma cells. Cancer Rep (Hoboken) 2020-07-29 [PMID: 32721127]
• Kim SB, Cho J, Jue SS et al. Effect of Hyaluronic Acid Filler Injection on the Interdental Papilla in a Mouse Model of Open Gingival Embrasure Int J Environ Res Public Health 2020-07-09 [PMID: 32660062] (IHC-P, Mouse)
• Liu J, Zhu YM, Guo Y et al. Inhibition of GSK3 Beta and RIP1K Attenuates Glial Scar Formation Induced by Ischemic Stroke via Reduction of Inflammatory Cytokine Production Front Pharmacol 2020-06-12 [PMID: 32595496]
• Tekatas A, Tekatas D. D, et al. Small fiber neuropathy and related factors in patients with systemic lupus erythematosus; the results of cutaneous silent period and skin biopsy. Adv Rheumatol 2020-06-05 [PMID: 32503623] (IHC-P, Human)
• Lv Y, Cang W, Li Q et al. Erlotinib overcomes paclitaxel-resistant cancer stem cells by blocking the EGFR-CREB/GR beta-IL-6 axis in MUC1-positive cervical cancer Oncogenesis 2019-11-26 [PMID: 31772161] (ELISA, IF/IHC, KD, Mouse, Human)
• Tessier S, Tran VA, Ottone OK et al. TonEBP-deficiency accelerates intervertebral disc degeneration underscored by matrix remodeling, cytoskeletal rearrangements, and changes in proinflammatory gene expression Matrix Biol. 2019-11-07 [PMID: 31707045] (IHC-P, Mouse)
• Chow S K, Chim Y N et al. Vibration treatment modulates macrophage polarisation and enhances early inflammatory response in oestrogen-deficient osteoporotic-fracture healing. Eur Cell Mater 2019-07-11 [PMID: 31697398] (IF/IHC, Rat)
• Bao Z, Fan L, Zhao L et al. Silencing of A20 Aggravates Neuronal Death and Inflammation After Traumatic Brain Injury: A Potential Trigger of Necroptosis Front. Mol. Neurosci. 2019-09-19 [PMID: 31607859] (WB, Rat)
• Chen M, Reed RR, Lane AP Chronic Inflammation Directs an Olfactory Stem Cell Functional Switch from Neuroregeneration to Immune Defense Cell Stem Cell 2019-09-04 [PMID: 31523027] (ICC/IF, Mouse)
• Bach A, Conzen C, Schubert GA et al. Acute changes of pro-inflammatory markers and corticosterone in experimental subarachnoid haemorrhage: A prerequisite for severity assessment PLoS ONE 2019-07-30 [PMID: 31361786] (WB, Rat)
• Yan H, Hu Y, Akk A et al. Interleukin-12 and -23 blockade mitigates elastase-induced abdominal aortic aneurysm Sci Rep 2019-07-18 [PMID: 31320700] (IF/IHC, Human, Mouse)
• Valzania L, Mattee M, Strand M, Brown M Analysis of inflammatory cytokine expression in the urinary tract of BALB/c mice infected with Proteus (P.) mirabilis and enteroaggregative Escherichia (E.) coli (EAEC) strains Folia Microbiol. (Praha) 2019-05-19 [PMID: 31104302] (IF/IHC, Mouse)
• Adhikari, N;Neupane, S;Aryal, YP;Choi, M;Sohn, WJ;Lee, Y;Jung, JK;Ha, JH;Choi, SY;Suh, JY;Kim, JY;Rho, MC;Lee, TH;Yamamoto, H;An, CH;Kim, SH;An, SY;Kim, JY; Effects of oleanolic acid acetate on bone formation in an experimental periodontitis model in mice J. Periodont. Res. 2019-04-15 [PMID: 30982986] (IF/IHC, Mouse)
• Yue S, Zheng X, Zheng Y. Cell type-specific role of lamin-B1 and its inflammation-driven reduction in organ building and aging Circulation Research 1905-07-10 [PMID: 30968547] (WB, Mouse)
• Novais EM, Diekman BO, Shapiro IM, Risbud MV. p16Ink4a deletion in cells of the intervertebral disc affects their matrix homeostasis and senescence associated secretory phenotype without altering onset of senescence Matrix Biol. 2019-02-24 [PMID: 30811968] (IHC-P, Mouse)
• Gurung S, Reuter N, Preno A et al. Zika virus infection at mid-gestation results in fetal cerebral cortical injury and fetal death in the olive baboon PLoS Pathog. 2019-01-01 [PMID: 30657788] (IHC-Fr, Monkey)
• Urak KT, Blanco GN, Shubham S et al. RNA inhibitors of nuclear proteins responsible for multiple organ dysfunction syndrome Nat Commun 2019-01-10 [PMID: 30631065] (ICC/IF, Mouse)
• Gorth DJ, Shapiro IM, Risbud MV. Transgenic mice overexpressing human TNF-a experience early onset spontaneous intervertebral disc herniation in the absence of overt degeneration. Cell Death Dis 2018-12-18 [PMID: 30584238] (ICC/IF, Mouse)
• Wang Q, He Z, Huang M et al. Vascular niche IL-6 induces alternative macrophage activation in glioblastoma through HIF-2a. Nat Commun. [PMID: 29422647] (IF/IHC, Mouse)
• Kaito T, Morimoto T, Mori Y et al. BMP-2/7 heterodimer strongly induces bone regeneration in the absence of increased soft tissue inflammation. Spine J 2018-01-01 [PMID: 28735764]
• Cullis J, Siolas D, Avanzi A, Barui S. Macropinocytosis of Nab-paclitaxel Drives Macrophage Activation in Pancreatic Cancer. Cancer Immunol Res. 2017-03-01 [PMID: 28108630] (WB, Mouse)
• Rossi AF, Cadamuro AC, Biselli-Perico JM et al. Interaction between inflammatory mediators and miRNAs in Helicobacter pylori infection. Cell. Microbiol. 2016-03-04 [PMID: 26945693] (IHC-P, Human)
• Kleme ML, Sane AT, Garofalo C, Levy E. Targeted CFTR gene disruption with Zinc-finger nucleases in human intestinal epithelial cells induces oxidative stress and inflammation. Int. J. Biochem. Cell Biol. 2016-02-25 [PMID: 26923293] (WB, Human)
  
  Details:
  TLR4 Inhibitor Peptide Set was used for functional assays in experiments involving human first-trimester trophoblast cells (Sw.71), isolated from a normal 7-week placenta. The cells were stimulated with either advanced glycation end products/AGEs or high-mobility group box-1/HMGB1 in the absence or presence of either RAGE antagonist FPS-XM1 or TLR4 inhibitor peptides.
• Ko AR, Jin DK, Cho SY et al. AAV8-mediated expression of N-acetylglucosamine-1-phosphate transferase attenuates bone loss in a mouse model of mucolipidosis II Mol. Genet. Metab. 2016-02-03 [PMID: 26857995] (IHC-P, Mouse)
• Poehler AM, Xiang W, Spitzer P et al. Autophagy modulates SNCA/alpha-synuclein release, thereby generating a hostile microenvironment. Autophagy 2014-01-01 [PMID: 25484190] (WB)
• Bai H, Liu X, Lin M et al. Progressive Senescence Programs Induce Intrinsic Vulnerability to Aging-related Breast Cancer bioRxiv 2023-05-10 (IHC, Mouse)
• Zhang W, Mottillo EP, Zhao J et al. Adipocyte Lipolysis Stimulated Interleukin-6 Production Requires Sphingosine kinase 1 activity. J Biol Chem. 2014-09-24 [PMID: 25253697]
• Lin FY, Hsiao FP, Huang CY et al. Porphyromonas gingivalis GroEL Induces Osteoclastogenesis of Periodontal Ligament Cells and Enhances Alveolar Bone Resorption in Rats. PLoS ONE 2014-07-25 [PMID: 25058444] (IHC-P, Rat)
  
  Details:
  IL6 antibody used in IHC-P for detecting the inflammation of the gingival connective tissue of Rats inoculated or not with Porphyromonas gingivalis GroEL (inoculated into rat gingiva) - Figure 5B.
• Bae EJ, Lee HJ, Rockenstein E et al. Antibody-Aided Clearance of Extracellular alpha-Synuclein Prevents Cell-to-Cell Aggregate Transmission J Neurosci 2012-09-26 [PMID: 23015436] (IF/IHC, Mouse)
• Cammarota R, Bertolini V, Pennesi G et al. The tumor microenvironment of colorectal cancer: stromal TLR-4 expression as a potential prognostic marker. J Transl Med. 2010-11-08 [PMID: 21059221] (IHC-P, Human)
• Bottino, R et al. Response of Human Islets to Isolation Stress the Effect of Antioxidant Treatment. Diabetes 53: 2559-2568. 2004-01-01 [PMID: 15448084]

Review for IL-6 Antibody (NB600-1131) (1)

Average Rating: 1

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Lenka Uherková Simple Western Rat 02/12/2020

Summary

• Application: Simple Western
• Sample Tested: skin wound - healthy
• Species: Rat
• Lot: 38801

Comments

• Comments: ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***

FAQs for IL-6 Antibody (NB600-1131). (Showing 1 - 2 of 2 FAQs).

1. I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
   • We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.
2. I had a quick question regarding NB600-1131SS IL-6 antibody. Not sure if you guys would be able to answer but how low do you think I could go on protein amount loaded to pick up a band? I see on the data sheet you guys have loaded 50ug and I cant load that much based on the nature of my samples.
   • We usually recommend anywhere from 10-50 ug/lane of protein. I wouldn't recommend using much lower than 10 ug of sample.

Bioinformatics

• Gene Symbol: IL6
• Entrez:
  • 3569(Human)
• Uniprot:
  • P05231()
  • P05231()
  • P05231()
  • P05231()
  • P05231()