INCA Antibody

Western Blot: INCA Antibody [NBP1-46144] - NBP1-46144 (0.5ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

INCA Antibody Summary

• Immunogen: Peptide with sequence C-NHLTTQDSQIVLPS corresponding to C-Terminus according to NP_001007233.1.
• Epitope: C-Terminus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CARD17
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.5 - 1.5 ug/ml
• Application Notes: WB: Approx. 15 kDa band double band observed in human kidney and lung lysates (calculated MW of 11.9 kDa band according to NP_001007233.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for INCA Antibody

• caspase recruitment domain family, member 17
• caspase recruitment domain-containing protein 17
• Caspase-1 inhibitor INCA
• INCAInhibitory CARD
• inhibitory caspase recruitment domain (CARD) protein
• Inhibitory caspase recruitment domain protein

Background

INCA, also known as CAR17 or Caspase recruitment domain-containing protein 17, is a 110 amino acid protein that is 12 kDa; cytoplasm located; regulates procaspase-1/CASP1 activation involved in the regulation of the proteolytic maturation of pro-IL-1beta/IL1B and its release during inflammation; and acts as an inhibitor of the release of IL1B in response to LPS in monocytes. Current research is being pursued on this protein involvement in several diseases and disorders including cancer, familial mediterranean fever, shigellosis, cowpox, neurodegenerative disease, vascular disease, mevalonic aciduria, tularemia, dermatitis, lung disease, legionnaires' disease, and arthritis. This protein has been linked to the proteolysis and regulation of apoptotic process pathways where it interacts with CASP1, CARD16, and CARD18 proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for INCA Antibody (NBP1-46144)(1)

Publication using NBP1-46144

• Lamkanfi M, Denecker G, Kalai M et al. INCA, a novel human caspase recruitment domain protein that inhibits interleukin-1beta generation J Biol Chem 2004-12-10 [PMID: 15383541]

Bioinformatics

• Gene Symbol: CARD17
• Entrez:
  • 440068(Human)
• Uniprot:
  • NP_001007233.1(Human)