iNOS Antibody - BSA Free

Western Blot: iNOS Antibody [NBP1-97471] - Rat brain extract, probed with iNOS, pAb.

• Reactivity: Mu, Rt, Bv
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

iNOS Antibody - BSA Free Summary

• Immunogen: iNOS Antibody was made to a synthetic peptide corresponding to amino aicds 1131-1144 of mouse iNOS.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NOS2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:1000
• Theoretical MW: 131 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for iNOS Antibody - BSA Free

• EC 1.14.13.39
• Hepatocyte NOS
• HEP-NOS
• Inducible NO synthase
• Inducible NOS
• iNOS
• nitric oxide synthase 2, inducible
• nitric oxide synthase 2A (inducible, hepatocytes)
• nitric oxide synthase, inducible
• nitric oxide synthase, macrophage
• NOS type II
• NOS
• NOS2
• NOS2A
• Peptidyl-cysteine S-nitrosylase NOS2

Background

Nitric oxide (NO) is a colorless, free radical gas that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family including the two constitutive isoforms: brain, bNOS, or neuronal NOS, nNOS (type I) and endothelial cell NOS, eNOS (type III); along with the inducible isoform, iNOS (type II). NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO, requiring the cofactors calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN), heme, and tetrahydrobiopterin (1).

The 131 kDa enzyme, iNOS, is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. While constitutively expressed in kidneys, in other tissues iNOS is induced by bacterial lipopolysaccharides (LPS), growth factors, and cytokines such as IFN-gamma, TNF, IL-1 and IL-2. iNOS is not regulated by the level of intracellular Ca2+ and is constantly active as a dimer when expressed. iNOS activity is elevated in a variety of diseases including atherosclerosis, heart failure, sepsis, solid tumors, and type 2 diabetes. Acting as a critical mediator of inflammation and apoptosis, iNOS inhibitors have been shown to alleviate obesity and stress inducted insulin resistance in mouse models (2,3).

References

1. Forstermann U, and Sessa WC. (2012) Nitric oxide synthases: regulation and function. Eur Heart J. 33(7): 829-837. PMID: 21890489

2. Aktan F. (2004) iNOS-mediated nitric oxide production and its regulation. Life Sci. 75(6):639-53. PMID: 15172174

3. Cinelli MA, Do HT, Miley GP, Silverman RB. (2020) Inducible nitric oxide synthase: Regulation, structure, and inhibition. Med Res Rev. 40(1):158-189. PMID: 31192483

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for iNOS Antibody (NBP1-97471)(6)

Publications using NBP1-97471

• Zeng Y, Liu Z, Tan X, Lei L The GPR55 antagonist CID16020046 mitigates advanced glycation end products (AGEs)- induced chondrocyte activation Chem. Biol. Interact. 2020-05-01 [PMID: 32360554] (WB, Mouse)
• Ben Menachem-Zidon O, Parkes I, Chill Hh et Al. Age-associated differences in macrophage response in a vaginal wound healing rat model Int Urogynecol J 2020-02-27 [PMID: 32108248]
• Jeong HG, Cha BG, Kang DW et al. Ceria Nanoparticles Fabricated with 6-Aminohexanoic Acid that Overcome Systemic Inflammatory Response Syndrome Adv Healthc Mater 2019-03-06 [PMID: 30843374] (WB, Mouse)
• M Oike et al. Proapoptotic nitric oxide production in amyloid beta protein-treated cerebral microvascular endothelial cells. Microcirculation 14, 89 . 2007-01-01 [PMID: 17365664] (WB, Bovine)
• Zheng Y, Yan S, Qi J et al. Protective effect of chitosan oligosaccharide against oxidative damage of peripheral blood mononuclear cells in dairy cows induced by diethylenetriamine/nitric oxide via NF-kappp B signalling pathway Italian Journal of Animal Science 2020-12-14 (WB, Bovine)
• Zheng Y, Zhang B, Qi J et al. Dietary supplementation of chitosan affects milk performance, markers of inflammatory response and antioxidant status in dairy cows Animal Feed Science and Technology 2021-04-01

FAQs for iNOS Antibody (NBP1-97471). (Showing 1 - 4 of 4 FAQs).

1. We need to evaluate the level of iNOS in Rat sciatic nerve by Western Blot. I see that you have few antibodies suitable for that, could you please recommend the best what you have for our purposes?
   • We have 4 iNOS antibodies that are suitable for your experiment. The best choice for you will depend on your specific preferences, in this case the main difference will be the immunogen location. If the immunogen is not important to you, then I would recommend choosing NB300-605 as it gives you the best value and we are able to provide the exact immunogen.
2. Would you please confirm the concentration of the current lot of this Ab: iNOS Antibody (NBP1-97471) - 0.05mg
   • The antibody is supplied at 1mg/ml.
3. What is the concentration of NBP1-97471?
   • The concentration for this product (lot 01061105) is 1.0mg/ml.
4. I used NBP1-97471 antibody to run WB on cow mammary epithelial cells treated by LPS. I used 60ug/lane, 10% gel, and I found there was no specific band. Would you please give some advice?
   • I think you can try running slightly less protein/lane. We usually use 20-30ug, so you can try 30-40ug instead of 60ug. The 10% gel should be fine, but if you want to separate the higher weights more, you can try using a lower percentage gel.

Bioinformatics

• Gene Symbol: NOS2
• Entrez:
  • 282876(Bovine)
  • 18126(Mouse)
  • 24599(Rat)