Integrin alpha 11 Antibody [Unconjugated]

Western blot shows lysates of mouse embryo tissue. PVDF Membrane was probed with 1 µg/mL of Mouse Integrin a11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6498) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Integrin a11 at approximately 230 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Integrin alpha 11 was detected in immersion fixed irradiated mouse embryonic fibroblasts (iMEF) using Mouse Integrin alpha 11 Affinity-purified Polyclonal Antibody (Catalog # AF6498) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Integrin alpha 11 Antibody [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin alpha 11
  Phe19-Pro1141
  Accession # P61622
• Specificity: Detects mouse Integrin alpha 11 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human (rh) Integrin alpha 11 is observed, and less than 2% cross-reactivity with recombinant mouse (rm) Integrin alpha 2 and rmIntegrin alpha 10 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: ITGA11
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin alpha 11 Antibody [Unconjugated]

• HsT18964
• Integrin alpha 11
• integrin alpha-11
• integrin, alpha 11
• ITGA11

Background

Integrin alpha 11 ( ITGA11) is a 155-160 kDa member of the integrin alpha chain family of molecules. It preferentially forms a cell surface heterodimer with beta 1 Integrin. In particular, it is further classified as a collagen-binding group member, showing a preference for binding to collagen I and II, plus group A Streptococcal Scl1 protein. ITGA11 is expressed by embryonic mesenchymal cells in areas that incorporate collagen during development. In adult tissue, fibroblasts express ITGA11, and a collagen:ITGA11 interaction likely contributes to the formation of myofibroblasts. Mature mouse ITGA11 is an 1166 amino acid (aa) type I transmembrane glycoprotein. It contains a large 1119 aa extracellular domain (ECD) (aa 23-1141) plus a short 24 aa cytoplasmic region. The ECD shows 7 x 60 aa FG-Gap repeats that generate beta -propellers (aa 24-650) with an intervening I domain that binds collagen (aa 164-345). There is one potential splice variant that shows an eight aa substitution for aa 89-119 coupled to a seven aa insertion after Gly690. Over aa 23-1141, mouse ITGA11 shares 95% and 90% aa identity with rat and human ITGA11, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin alpha 11 Antibody (AF6498)(2)

Publications using AF6498

• Jahedul Alam, Moses Musiime, Andreas Romaine, Mugdha Sawant, Arne Olav Melleby, Ning Lu et al. Generation of a novel mouse strain with fibroblast-specific expression of Cre recombinase Matrix Biology Plus 2020-11-01 [PMID: 33543038]
• Grassot V, Da Silva A, Saliba J, Maftah A, Dupuy F, Petit J Highlights of glycosylation and adhesion related genes involved in myogenesis. BMC Genomics, 2014-07-22;15(0):621. 2014-07-22 [PMID: 25051993] (Neutralization, Western Blot, Mouse)

Bioinformatics

• Gene Symbol: ITGA11
• Uniprot:
  • P61622(Mouse)