IRF1 Antibody - BSA Free

Western Blot: IRF1 Antibody [NBP1-78761] - Whole cell lysate from NIH3T3 cells (M; 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded). Antibodies: Affinity purified rabbit anti-IRF1 antibody used for WB at 0.2 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. IRF1 was also immunoprecipitated by rabbit anti-IRF1 antibodies NBP1-78762 and NBP1-78763 which recognize downstream epitopes.

• Reactivity: Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

IRF1 Antibody - BSA Free Summary

• Immunogen: The immunogen for this product maps to a region between residue 200 and 250 of mouse Interferon Regulatory Factor 1 using the numbering given in entry NP_032416.1 (GeneID 16362).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IRF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for IRF1 Antibody - BSA Free

• interferon regulatory factor 1
• IRF1
• MAR
• MARIRF-1

Background

IRF1 encodes interferon regulatory factor 1, a member of the interferon regulatory transcription factor (IRF) family. IRF1 serves as an activator of interferons alpha and beta transcription, and in mouse it has been shown to be required for double-stranded RNA induction of these genes. IRF1 also functions as a transcription activator of genes induced by interferons alpha, beta, and gamma. Further, IRF1 has been shown to play roles in regulating apoptosis and tumor-suppressoion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IRF1
• Entrez:
  • 16362(Mouse)
• Uniprot:
  • P15314()