IRF3 Antibody - BSA Free

Western Blot: IRF3 Antibody [NBP1-78769] - Whole cell lysate from mouse bone marrow dendritic (D; 50 and 15 mcg for WB; 1 mg for IP, 20% of IP loaded), and NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-IRF3 antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. IRF3 was also immunoprecipitated by rabbit anti-IRF3 antibody NBP1-78768 which recognizes a disparate epitope.

• Reactivity: Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

IRF3 Antibody - BSA Free Summary

• Immunogen: The immunogen for this product maps to a region between residue 375 and 419 of mouse Interferon Regulatory Factor 3 using the numbering given in entry NP_058545.1 (GeneID 54131).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IRF3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:1000-1:2500
• Application Notes: Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for IRF3 Antibody - BSA Free

• interferon regulatory factor 3
• IRF3
• IRF-3

Background

IRF3 is a family of interferon (IFN) regulatory factors (IRFs) have been shown to play a role in transcription of IFN genes as well as IFN-stimulated genes. The IRF-3 gene encodes a 50-kDa protein that binds specifically to the IFN-stimulated response element (ISRE) but not to the IRF-1 binding site PRD-I. Overexpression of IRF-3 stimulates expression of the IFN-stimulated gene 15 (ISG15) promoter, an ISRE-containing promoter. he high amino acid homology between IRF-3 and ISG factor 3 gamma polypeptide (ISGF3 gamma) and their similar binding properties indicate that, like ISGF3 gamma, IRF-3 may activate transcription by complex formation with other transcriptional factors, possibly members of the Stat family (1). IRF-3 is expressed constitutively in a variety of tissues, and the relative levels of IRF-3 mRNA do not change in virus-infected or IFN-treated cells. (2). In uninfected cells, the IRF-3 component of DRAF1 resides in the cytoplasm. The cytoplasmic localization of IRF-3 is dependent on a nuclear export signal, and IRF-3 is recognized by the chromosome region maintenance 1 (CRM1) (also known as exportin 1) shuttling receptor. Following infection and specific phosphorylation, IRF-3 accumulates in the nucleus where it associates with CBP and p300 (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IRF3 Antibody (NBP1-78769)(3)

Publications using NBP1-78769

• Banerjee D, Langberg K, Abbas S Et al. A non-canonical, interferon-independent signaling activity of cGAMP triggers DNA damage response signaling Nature communications 2021-10-27 [PMID: 34707113] (WB, Mouse)
• Glanz A, Chakravarty S, Fan S Et al. Autophagic degradation of IRF3 induced by the small-molecule auranofin inhibits its transcriptional and proapoptotic activities The Journal of biological chemistry 2021-10-05 [PMID: 34619149]
• N Kerur, S Fukuda, D Banerjee, Y Kim, D Fu, I Apicella, A Varshney, R Yasuma, BJ Fowler, E Baghdasary, KM Marion, X Huang, T Yasuma, Y Hirano, V Serbulea, M Ambati, VL Ambati, Y Kajiwara, K Ambati, S Hirahara, A Bastos-Car, Y Ogura, H Terasaki, T Oshika, KB Kim, DR Hinton, N Leitinger, JC Cambier, JD Buxbaum, MC Kenney, SM Jazwinski, H Nagai, I Hara, AP West, KA Fitzgerald, SR Sadda, BD Gelfand, J Ambati cGAS drives noncanonical-inflammasome activation in age-related macular degeneration Nat. Med., 2017-11-27;0(0):. 2017-11-27 [PMID: 29176737] (, )

Bioinformatics

• Gene Symbol: IRF3
• Entrez:
  • 54131(Mouse)
• Uniprot:
  • P70671()