IRF7 Antibody - BSA Free

Western Blot: IRF7 Antibody [NBP1-77263] - 293 whole cell lysate with IRF7 antibody at (A) 0.5 and (B) 1 ug/ml and (C) 2 ug/ml.

Immunocytochemistry/Immunofluorescence: IRF7 Antibody [NBP1-77263] - N9 cells stained for IRF-7 (Dilution 1:200). Image from verified customer review.

Immunohistochemistry: IRF7 Antibody - BSA Free [NBP1-77263] - Immunohistochemistry of IRF7 in human spleen tissue with IRF7 Antibody at 5 ug/mL.

Immunocytochemistry/ Immunofluorescence: IRF7 Antibody - BSA Free [NBP1-77263] - Immunofluorescence of IRF7 in Mouse Spleen cells with IRF7 antibody at 20 ug/mL.

Immunohistochemistry: IRF7 Antibody - BSA Free [NBP1-77263] - Immunohistochemistry of IRF7 in mouse spleen tissue with IRF7 antibody at 5 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

IRF7 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human IRF7. The immunogen is located within amino acids 420-470 of IRF7. Amino Acid Squence: GQDLSAGRPKEKSL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IRF7
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 0.5-1 ug/ml
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  IRF7 Peptide (NBP1-77263PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for IRF7 Antibody - BSA Free

• interferon regulatory factor 7
• interferon regulatory factor-7H
• IRF-7
• IRF7A
• IRF-7H

Background

Interferons (IFNs) are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms. During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-a/b genes. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. IRF7 play a major role in the innate immune pathway, interacting with the Toll-like receptor (TLR) adaptor proteins MyD88 and Tirp/TRAM and functioning as an intermediate TLR4 and TLR9 signaling. There are at least four differentially spliced isoforms of IRF7, although their function has not been clearly established.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IRF7 Antibody (NBP1-77263)(2)

Publications using NBP1-77263

• Xiang Z, Liu L, Lei X et al. The 3C Protease of Enterovirus D68 Inhibits the Cellular Defense Mediated by Interferon Regulatory Factor 7. J. Virol. 2015-11-25 [PMID: 26608321] (WB, Human)
• Mboko WP, Mounce BC, Wood BM, Kulinski JM, Corbett JA, Tarakanova VL. Coordinate regulation of DNA damage and type I interferon responses imposes an antiviral state that attenuates mouse gammaherpesvirus type 68 replication in primary macrophages. J Virol;86(12):6899-912. 2012-06-01 [PMID: 22496235] (WB, Mouse)

Review for IRF7 Antibody (NBP1-77263) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer ICC Mouse 04/07/2023

Summary

• Application: Immunocytochemistry
• Sample Tested: N9 cell line
• Species: Mouse
• Lot: 3941-1901

Bioinformatics

• Gene Symbol: IRF7
• Entrez:
  • 3665(Human)
• Uniprot:
  • Q92985(Human)