JunD Antibody (512709)

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, 293T human embryonic kidney cell line, Jurkat human acute T cell leukemia cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse JunD Monoclonal Antibody (Catalog # MAB5526) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for JunD at approximately 40 kDa and 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu
• Applications: WB
• Clone: 512709
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

JunD Antibody (512709) Summary

• Immunogen: E. coli-derived recombinant human JunD
  Lys47-Gly156
  Accession # CAA40010
• Specificity: Detects endogenous human and mouse JunD in Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: JUND
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for JunD Antibody (512709)

• activator protein 1
• AP-1
• jun D proto-oncogene
• JunD
• JunD-FL isoform
• transcription factor jun-D

Background

JunD is a member of the Jun family of nuclear transcription factors. It dimerizes with other Jun or Fos family members to form the AP1 transcription factor. Unlike c‑Jun or JunB, JunD antagonizes Ras transformation and accumulates as cells enter quiescence. JunD is a 347 amino acid (aa), 39 kDa protein (JunD-FL) that has a 303 aa, 34 kDa isoform ( delta JunD) which begins at aa 44. Over the sequence used as an immunogen, human JunD shares 72% aa identity with mouse and rat JunD.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for JunD Antibody (MAB5526)(2)

Publications using MAB5526

• S Ghaffari, C Hanson, RE Schmidt, KJ Bouchonvil, SM Offer, S Sinha An integrated multi-omics approach to identify regulatory mechanisms in cancer metastatic processes Genome Biology, 2021-01-07;22(1):19. 2021-01-07 [PMID: 33413550] (Western Blot, Human)
• Kashiwada M, Cassel SL, Colgan JD NFIL3/E4BP4 controls type 2 T helper cell cytokine expression. EMBO J., 2011-04-15;30(10):2071-82. 2011-04-15 [PMID: 21499227] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: JUND
• Uniprot:
  • CAA40010()