Laminin S/Laminin beta 2 Antibody (CL2979) - BSA Free

Immunohistochemistry-Paraffin: Laminin S/Laminin beta 2 Antibody (CL2979) [NBP2-42387] - Staining in human heart muscle and tonsil tissues. Corresponding LAMB2 RNA-seq data are presented for the same tissues.

Western Blot: Laminin S/Laminin beta 2 Antibody (CL2979) [NBP2-42387] - Analysis of purified human recombinant Laminin-421, Laminin-521, Laminin-332 and Laminin-111.

Western Blot: Laminin S/Laminin beta 2 Antibody (CL2979) [NBP2-42387] - Analysis in human lung tissue.

Immunohistochemistry-Paraffin: Laminin S/Laminin beta 2 Antibody (CL2979) [NBP2-42387] - Staining of human heart muscle shows moderate membranous positivity in cardiomyocytes.

Immunohistochemistry-Paraffin: Laminin S/Laminin beta 2 Antibody (CL2979) [NBP2-42387] - Staining of human kidney shows moderate membranous positivity in basement membrane of cells in glomeruli.

Immunohistochemistry-Paraffin: Laminin S/Laminin beta 2 Antibody (CL2979) [NBP2-42387] - Staining of human endometrium shows moderate positivity in basement membrane of glandular cells.

Immunohistochemistry-Paraffin: Laminin S/Laminin beta 2 Antibody (CL2979) [NBP2-42387] - Staining of human tonsil shows no positivity in lymphoid cells as expected.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL2979
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Laminin S/Laminin beta 2 Antibody (CL2979) - BSA Free Summary

• Immunogen: This antibody was developed using a recombinant protein derived from P55268, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: LAMB2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:2500 - 1:5000
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reactivity Notes

Expected to cross react based on sequence identity: Rat (82%), Mouse (83%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for Laminin S/Laminin beta 2 Antibody (CL2979) - BSA Free

• LAMB2
• Laminin B1s chain
• Laminin beta 2
• Laminin S
• laminin subunit beta-2
• laminin, beta 2 (laminin S)
• Laminin-11 subunit beta
• Laminin-14 subunit beta
• Laminin-15 subunit beta
• Laminin-3 subunit beta
• Laminin-4 subunit beta
• Laminin-7 subunit beta
• LAMSLaminin-9 subunit beta
• S-LAM beta
• S-laminin subunit beta

Background

The Laminins comprise a growing family of disulfide-linked heterotrimers consisting of three genetically distinct polypeptide chains, designated alpha, beta and gamma. A major component of the basal lamina, Laminins play a crucial role in providing a scaffolding upon which tissues are assembled and which serves as a physical barrier separating specialized tissues. During embryogenesis and early development, cells migrate along basement membranes, which are required for the polarization of cells. The beta-2 laminin chain plays a role in melanoma spread, promoting tumor migration along the abluminal surface of vessel, a phenomenon which has been termed extra-vascular migratory metastasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LAMB2
• Uniprot:
  • P55268(Human)