Lipocalin-1 Antibody (10B10)

Western Blot: Lipocalin-1 Antibody (10B10) [NBP1-28872] - analysis using anti-LCN1 monoclonal antibody against HEK293 (1) and LCN1-hIgGFc transfected HEK293 cell lysate(2).

Western Blot: Lipocalin-1 Antibody (10B10) [NBP1-28872] - analysis using anti-LCN1 monoclonal antibody against HEK293 (1) and LCN1-hIgGFc transfected HEK293 cell lysate(2).

Immunocytochemistry/Immunofluorescence: Lipocalin-1 Antibody (10B10) [NBP1-28872] - analysis of methanol-fixed HEK293 cells trasfected with LCN1-hIgGFc using anti-LCN1 monoclonal antioby(green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye

Immunohistochemistry-Frozen: Lipocalin-1 Antibody (10B10) [NBP1-28872] - Human lung tissue stained with Lipocalin-1 Antibody. IHC-Fr image submitted by a verified customer review.

Immunohistochemistry-Paraffin: Lipocalin-1 Antibody (10B10) [NBP1-28872] - Immunohistochemical analysis of paraffin-embedded human Prostate tissues using LCN1 mouse mAb

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 10B10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Lipocalin-1 Antibody (10B10) Summary

• Immunogen: Purified recombinant fragment of Lipocalin-1 expressed in E. Coli.
• Specificity: Specific for LCN1.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LCN1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 19.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for Lipocalin-1 Antibody (10B10)

• LCN1
• lipocalin 1 (tear prealbumin)
• lipocalin 1-like 2
• Lipocalin1
• Lipocalin-1
• MGC71975
• PMFA
• Tear lipocalin
• Tear Prealbumin
• TLC
• TPVon Ebner gland protein
• VEG protein
• VEGP
• VEGPlipocalin 1 (protein migrating faster than albumin, tear prealbumin)

Background

lipocalin 1 (tear prealbumin), also known as TP, PMFA, VEGP. Entrez Protein: NP_002288. The protein belongs to the lipocalin family. Lipocalins are a group of extracellular proteins that are able to bind lipophiles by enclosure within their structures to minimize solvent contact. This protein may bind hydrophobic ligands and inhibit cysteine proteinases. It may also play a role in taste reception and could be necessary for the concentration and delivery of sapid molecules in the gustatory system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Lipocalin-1 Antibody (NBP1-28872) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IHC-Fr Human 08/18/2021

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Lung tissue
• Species: Human
• Lot: 10B10

Comments

• Comments: ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***

Bioinformatics

• Gene Symbol: LCN1