LOX-1/OLR1 Antibody

Immunohistochemistry-Paraffin: LOX-1/OLR1 Antibody [NBP2-27298] - Formalin-fixed, paraffin-embedded human brain stained with LOX-1 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

LOX-1/OLR1 Antibody Summary

• Immunogen: A portion of amino acids 150-210 of human LOX-1 was used as the immunogen for the antibody. (aa155-171)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: OLR1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5-7ug/ml
• Application Notes: Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Use in Immunohistochemistry and Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 27519944)/

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 ml PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A purified

Alternate Names for LOX-1/OLR1 Antibody

• CLEC8A
• CLEC8ASLOX1
• C-type lectin domain family 8 member A
• hLOX-1
• Lectin-like oxidized LDL receptor 1
• Lectin-like oxLDL receptor 1
• Lectin-type oxidized LDL receptor 1
• LOX1
• LOX-1
• LOX1ox LDL receptor 1
• LOXIN
• OLR1
• oxidised low density lipoprotein (lectin-like) receptor 1
• oxidized low density lipoprotein (lectin-like) receptor 1
• oxidized low-density lipoprotein receptor 1
• oxidized low-density lipoprotein receptor 1, soluble form
• Ox-LDL receptor 1
• SCARE1
• scavenger receptor class E, member 1
• SR-E1

Background

CLEC8A, also called LOX-1 or OLR1, is one of the original C-Lectin type receptors (CLRs) associated with the fungal pattern recognition receptor Dectin-1. The DECTIN-1 associated clusters, including LOX-1, are found in the mammalian natural killer gene complex (NkC) (1). LOX-1 is an innate immune receptor expressed on a variety of cells including activated macrophages, platelets, smooth muscle endothelium and fibroblasts. Expression of LOX-1 is increased by pro-inflammatory milieus in autoimmune pathologies like diabetes or atherosclerosis or hyperlipidemia caused also by oxidative stress or oxLDL (2, 3). As activating immunoreceptors, CLRs are type II transmembrane proteins with a single CTLD, a C-type lectin-like domain that recognizes proteins and not carbohydrates (4). Antibody to LOX-1 is useful for functional, phenotyping or structural studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LOX-1/OLR1 Antibody (NBP2-27298)(1)

Publication using NBP2-27298

• Chen M, Qiu H, Lin X et al. Lectin-like oxidized low-density lipoprotein receptor (LOX-1) in sickle cell disease vasculopathy Blood Cells Mol Dis 2016-06-25 [PMID: 27519944] (ICC/IF, IF/IHC)

Bioinformatics

• Gene Symbol: OLR1
• Uniprot:
  • P78380()