MMP-9 Antibody - BSA Free

Western Blot: MMP-9 Antibody [NBP1-57940] - Western blot of MMP-9. Image from verified customer review.

Western Blot: MMP-9 Antibody [NBP1-57940] - Western blot of MMP-9. Image from verified customer review.

Immunohistochemistry-Paraffin: MMP-9 Antibody [NBP1-57940] - Human prostate cancer was detected using HRP/AEC red color stain. Dilution: 2-15 ug/mL.

Western Blot: MMP-9 Antibody [NBP1-57940] - Analysis of MMP-9 in mouse brain tumor lysate (GL261 implanted mouse glioma tissue, 40 ug/lane) using anti-MMP-9 antibody. Image from verified customer review.

Western Blot: MMP-9 Antibody [NBP1-57940] - HeLa cell lysate. Antibody at 1.25 ug/mL.

Western Blot: MMP-9 Antibody [NBP1-57940] - Human Fetal Lung. Antibody at 1.0 ug/mL.

• Reactivity: Hu, Mu, Rt, Sh
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

MMP-9 Antibody - BSA Free Summary

• Immunogen: Synthetic peptide corresponding to the N-terminal of the human MMP-9 protein (between amino acids 150-200) UniProt P14780.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MMP9
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:250
  • Western Blot 1.25 ug/mL
• Theoretical MW: 78 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MMP-9 Antibody - BSA Free

• 92 kDa gelatinase
• 92 kDa type IV collagenase
• CLG4B
• EC 3.4.24
• EC 3.4.24.35
• Gelatinase B
• GELB
• macrophage gelatinase
• MANDP2
• matrix metallopeptidase 9
• matrix metalloproteinase 9
• matrix metalloproteinase-9
• MMP9
• MMP-9
• type V collagenase

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by MMP9 degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MMP-9 Antibody (NBP1-57940)(10)

Publications using NBP1-57940

• Cengel Kurnaz S, Kuruca N, GUvenC D et al. Topical Administration of Carvacrol Improves Healing in Nasal Septal Perforation: An Experimental Animal Study American journal of rhinology & allergy 2022-03-03 [PMID: 35238647] (IF/IHC)
• Kollaras V, Valsami G, Lambropoulou M et al. Effect of silibinin on the expression of MMP2, MMP3, MMP9 and TIMP2 in kidney and lung after hepatic ischemia/reperfusion injury in an experimental rat model Acta Cirúrgica Brasileira 2021-11-08 [PMID: 34755764] (Western Blot, Human)
• Vuong TT, Ronning SB, Kolset SO, Pedersen ME. The enzyme profiles in the connective tissue attaching pin bones to the surrounding tissue is specific in farmed salmon (Salmo salar) and cod (Gadus morhua L.) Fish Physiol. Biochem. 2016-07-09 [PMID: 27394140] (IHC-P)
• Vaithilingam V, Quayum N, Joglekar MV et al. Effect of alginate encapsulation on the cellular transcriptome of human islets. Biomaterials. 2011-09-01 [PMID: 21889795] (WB, Human)
• Cengel Kurnaz S, Aksoy A, Guvenc D. Does Resveratrol Have a Protective Effect on Tympanosclerosis Formation in Experimentally Induced Otitis Media? Int Adv Otol 2 2014-01-01 (IHC-P, Rat)
• StacY MR, Best CA, Maxfield MW et al. Magnetic Resonance Imaging of Shear Stress and Wall Thickness in Tissue-Engineered Vascular Grafts. Tissue Eng Part C Methods. 2018-07-31 [PMID: 29978768] (IHC-P, Sheep)
• De Cunto G, Lamberti A, de Santi MM et al. Middermal Elastolysis: Dermal Fibroblasts Cooperate with Inflammatory Cells to the Elastolytic Disorder Mediators Inflamm. 2017-09-16 [PMID: 29097850] (IF/IHC, Human)
• Su C Tanshinone IIA decreases the migratory ability of AGS cells by decreasing the protein expression of matrix metalloproteinases, nuclear factor kappa B-p65 and cyclooxygenase-2. Mol Med Rep 2016-01-02 [PMID: 26648518] (WB, Human)
• Xu B, Guenther JF, Pociask DA et al. Promotion of Lung Tumor Growth by Interleukin-17. Am. J. Physiol. Lung Cell Mol. Physiol. 2014-07-18 [PMID: 25038189] (IHC-P, Mouse)
  
  Details:
  MMP9 antibody used for IHC-P on lungs of K-RasLA1 mice (C57BL/6 background) - autochthonous/lung tumor model (lung delivery of a recombinant adenovirus that expresses IL-17A). Primary dilution 1:200, ON 4C incubation, HRP-DAB detection, IHC images in Fig. 3C.
• Bellini T, Trentini A, Manfrinato MC, Tamborino C, Volta CA, Di Foggia V, Fainardi E, Dallocchio F, Castellazzi M. Matrix metalloproteinase-9 activity detected in body fluids is the result of two different enzyme forms. J Biochem;151(5):493-499. 2012-05-01 [PMID: 22343748] (WB, Human)

Reviews for MMP-9 Antibody (NBP1-57940) (4)

Average Rating: 4.3

(Based on 4 reviews)

We have 4 reviews tested in 2 species: Human, Mouse.

reviewed by: Emily Thompson WB Human 12/13/2019

Summary

• Application: Western Blot
• Sample Tested: conditioned media from human glioma cells,conditioned media from U251 human glioma cell line
• Species: Human
• Lot: A-2

reviewed by: Emily Thompson WB Human 05/02/2019

Summary

• Application: Western Blot
• Sample Tested: concentrated conditioned media,U251 glioma cell line; concentrated conditioned media
• Species: Human
• Lot: A-2

reviewed by: Verified Customer WB Mouse 12/12/2014

Summary

• Application: Western Blot
• Sample Tested: GL261 implanted mouse glioma tissue
• Species: Mouse
• Lot: A

reviewed by: Verified Customer WB Mouse 07/21/2014

Summary

• Application: Western Blot
• Sample Tested: Mouse glioma tissue
• Species: Mouse
• Lot: QC3458

FAQs for MMP-9 Antibody (NBP1-57940). (Showing 1 - 2 of 2 FAQs).

1.  I’m looking for a pair of antibodies to MMP-9 that can be used in a sandwich assay. Do you carry any?   ..show answer..
   • We have 10 primary antibodies for MMP-9 that have been tested in ELISA, seen here.It looks like 2 have been tested for capture (please note the tested species for each of these), seen here.6 have been tested for detection, seen here.
2. I have purchased MMP-9 NBP1-57940 and it is lyophilized. I would like to know how to dilute them for further processing.  ..show answer..
   • Our product NBP1-57940 is provided lyophilized. We recommend reconstituting with 0.1ml of dH2O.

Bioinformatics

• Gene Symbol: MMP9
• Entrez:
  • 4318(Human)
• Uniprot:
  • P14780()