Mouse IL-1 beta/IL-1F2 Quantikine HS ELISA Kit

• Reactivity: Mu
• Applications: ELISA
• Conjugate: HRP

Mouse IL-1 beta/IL-1F2 Quantikine HS ELISA Kit Summary

• Background: The Quantikine^® HS Mouse IL-1 beta /IL-1F2 Immunoassay is a 4.0 hour solid phase ELISA designed to measure mouse IL-1 beta levels in serum and plasma. It contains E. coli-expressed recombinant mouse IL-1 beta and antibodies raised against the recombinant protein. Results obtained using natural IL-1 beta showed linear curves that were parallel to the standard curves obtai...ned using the Quantikine^® HS kit standards. These results indicate that the Quantikine^® HS Immunoassay kit can be used to determine relative mass values for natural mouse IL-1 beta .
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• Specificity: Natural and recombinant mouse IL-1 beta
• Source: N/A
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse IL-1 beta/IL-1F2 Quantikine HS ELISA Kit

• catabolin
• IL1 beta
• IL-1 beta
• IL-1
• IL1B
• IL-1b
• IL1-BETA
• IL-1F2
• IL1F2IL-1 beta
• interleukin 1, beta
• interleukin-1 beta
• preinterleukin 1 beta
• pro-interleukin-1-beta

Background

The Interleukin 1 (IL-1) family of proteins consists of IL-1 alpha , IL-1 beta , and the IL-1 receptor antagonist (IL-1ra). IL-1 alpha and IL-1 beta bind to the same cell surface receptors and share biological functions (1). IL-1 is not produced by unstimulated cells of healthy individuals with the exception of skin keratinocytes, some epithelial cells, and certain cells of the central nervous system. However, in response to inflammatory agents, infections, or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cell types is seen. IL-1 beta plays a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. Inappropriate or prolonged production of IL-1 has been implicated in a variety of pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulindependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases (2-5). 

 IL-1 alpha and IL-1 beta are structurally related polypeptides that show approximately 25% homology at the amino acid (aa) level. Both are synthesized as 31 kDa precursors that are subsequently cleaved into mature proteins of approximately 17.5 kDa (6, 7). Cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response (2, 8). Neither IL-1 alpha nor IL-1 beta contains a typical hydrophobic signal peptide (9-11), but evidence suggests that these factors can be secreted by non-classical pathways (12, 13). A portion of unprocessed IL-1 alpha can be presented on the cell membrane and may retain biological activity (14). The precursor form of IL-1 beta , unlike the IL-1 alpha precursor, shows little or no biological activity in comparison to the processed form (13, 15). Both unprocessed and mature forms of IL-1 beta are exported from the cell. 

 IL-1 alpha and IL-1 beta exert their effects through immunoglobulin superfamily receptors that additionally bind IL-1ra. The 80 kDa transmembrane type I receptor (IL-1 RI) is expressed on T cells, fibroblasts, keratinocytes, endothelial cells, synovial lining cells, chondrocytes, and hepatocytes (16, 17). The 68 kDa transmembrane type II receptor (IL-1 RII) is expressed on B cells, neutrophils, and bone marrow cells (18). The two IL-1 receptor types show approximately 28% homology in their extracellular domains but differ significantly in that the type II receptor has a cytoplasmic domain of only 29 aa, whereas the type I receptor has a 213 aa cytoplasmic domain. IL-1 RII does not appear to signal in response to IL-1 and may function as a decoy receptor that attenuates IL-1 function (19). The IL-1 receptor accessory protein (IL-1 RAcP) associates with IL-1 RI and is required for IL-1 RI signal transduction (20). IL-1ra is a secreted molecule that functions as a competitive inhibitor of IL-1 (21, 22). Soluble forms of both IL-1 RI and IL-1 RII have been detected in human plasma, synovial fluids, and the conditioned media of several human cell lines (23, 24). In addition, IL-1 binding proteins that resemble soluble IL-1 RII are encoded by vaccinia and cowpox viruses (25).

Publications for IL-1 beta/IL-1F2 (MHSLB00)(6)

Publications using MHSLB00

• B Yang, X Yang, X Sun, J Shi, Y Shen, R Chen IL-6 Deficiency Attenuates Skeletal Muscle Atrophy by Inhibiting Mitochondrial ROS Production through the Upregulation of PGC-1alpha in Septic Mice Oxidative Medicine and Cellular Longevity, 2022-04-27;2022(0):9148246. 2022-04-27 [PMID: 35528525] (Mouse)
• E Igel, A Haller, PR Wolfkiel, M Orr-Asman, A Jaeschke, DY Hui Distinct pro-inflammatory properties of myeloid cell-derived apolipoprotein E2 and E4 in atherosclerosis promotion The Journal of Biological Chemistry, 2021-08-21;0(0):101106. 2021-08-21 [PMID: 34425108] (Mouse)
• HJ Shin, SH Kim, HJ Park, MS Shin, I Kang, MJ Kang Nucleotide-binding domain and leucine-rich-repeat-containing protein X1 deficiency induces nicotinamide adenine dinucleotide decline, mechanistic target of rapamycin activation, and cellular senescence and accelerates aging lung-like changes Aging Cell, 2021-06-04;0(0):e13410. 2021-06-04 [PMID: 34087956] (Mouse)
• SY Cheon, BN Koo, SY Kim, EH Kam, J Nam, EJ Kim Scopolamine promotes neuroinflammation and delirium-like neuropsychiatric disorder in mice Scientific Reports, 2021-04-16;11(1):8376. 2021-04-16 [PMID: 33863952] (Mouse)
• I Cho, JM Kim, EJ Kim, SY Kim, EH Kam, E Cheong, M Suh, BN Koo Orthopedic surgery-induced cognitive dysfunction is mediated by CX3CL1/R1 signaling Journal of Neuroinflammation, 2021-04-15;18(1):93. 2021-04-15 [PMID: 33858422] (Mouse)
• J Lv, ZL Wu, Z Gan, P Gui, SL Yao CXCL14 Overexpression Attenuates Sepsis-Associated Acute Kidney Injury by Inhibiting Proinflammatory Cytokine Production Mediators Inflamm., 2020-03-31;2020(0):2431705. 2020-03-31 [PMID: 32317861] (Mouse)