NFATC1/NFAT2 Antibody (7A6)

Immunocytochemistry/Immunofluorescence: NFATC1/NFAT2 Antibody (7A6) [NB300-620] - NFATc1 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing NFATc1 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Immunohistochemistry-Paraffin: NFATC1/NFAT2 Antibody (7A6) [NB300-620] - Normal biopsies of deparaffinized Human spleen tissue.

Immunocytochemistry/Immunofluorescence: NFATC1/NFAT2 Antibody (7A6) [NB300-620] - NFATc1 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing NFATc1 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Immunocytochemistry/Immunofluorescence: NFATC1/NFAT2 Antibody (7A6) [NB300-620] - NFATc1 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing NFATc1 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Immunohistochemistry: NFATC1/NFAT2 Antibody (7A6) [NB300-620] - Normal biopsies of deparaffinized Human tonsil tissue.

Immunohistochemistry-Paraffin: NFATC1/NFAT2 Antibody (7A6) [NB300-620] - Normal biopsies of deparaffinized Human skeletal muscle tissue.

• Reactivity: Hu, Mu, Rt, Ha, Pm, Rb
• Applications: WB, Simple Western, GS, ICC/IF, IHC, IP, ChIP
• Clone: 7A6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

NFATC1/NFAT2 Antibody (7A6) Summary

• Immunogen: Bacterially expressed glutathione S-transferase (GST) fusion protein containing NF-ATc1 residues 1-654.
• Specificity: Detects cytosolic nuclear factor of activated T-cells (NFAT 2) from transfected human, mouse, and monkey cells. This does not cross react with NFATp.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NFATC1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • Gel Super Shift Assays 1:1 - 1:100
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:20 - 1:200
  • Immunohistochemistry-Paraffin 1:20 - 1:200
  • Immunoprecipitation 1:10 - 1:500
  • Simple Western
  • Western Blot 1:100 - 1:2000
• Application Notes: WB: Detects an approx. 105 kDa band representing transfected NFAT2 from COS cell lysate.
  See Simple Western Antibody Database for Simple Western validation: separated by size
• Control:

  NFATC1/NFAT2 Overexpression Lysate

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 16129432). Hamster reactivity reported in scientific literature (PMID: 10866678). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Ascites diluted with PBS
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for NFATC1/NFAT2 Antibody (7A6)

• MGC138448
• NFAT transcription complex cytosolic component
• NFAT2
• NFAT2cytoplasmic 1
• NFATC
• NF-ATC
• NFATC1
• NF-ATc1
• nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1

Background

The nuclear factor of activated T-cells (NFAT) transcription complex is required for the expression of a group of proteins that coordinate the immune response. NFAT2 is the cytosolic component of NFAT and is encoded by a separate gene. Expression of NFAT2 activates the interleukin (IL-2) promoter in non-T lymphocytes and is required for IL-2 gene expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NFATC1/NFAT2 Antibody (NB300-620)(4)

Publications using NB300-620

• Zhang P, Hu S, Xu H et al. Bushenhuoxue formula inhibits osteoclastogenesis and bone resorption on rabbit steroid-related osteonecrosis of the femoral head by suppressing NF-kappa B/NFATc1 pathway Research Square 2020-09-03 (WB, Human)
• Yu F, Li F, Yu P et al. Identification and characterization of NFATc1+ skeletal stem cells in bone regeneration Cell reports 2022-11-08 [PMID: 36351390] (Chemotaxis, Human)
• Zavaczki E, Gall T, Zarjou A et Al. Ferryl Hemoglobin Inhibits Osteoclastic Differentiation of Macrophages in Hemorrhaged Atherosclerotic Plaques Oxid Med Cell Longev 2020-02-27 [PMID: 32184915] (WB, ICC/IF, Mouse)
• Alpini G, Franchitto A, Demorrow S et al. Activation of alpha(1) -adrenergic receptors stimulate the growth of small mouse cholangiocytes via calcium-dependent activation of nuclear factor of activated T cells 2 and specificity protein 1 Hepatology 2011-02-01 [PMID: 21274883] (ICC/IF, IF/IHC, Mouse)

Review for NFATC1/NFAT2 Antibody (NB300-620) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer WB Mouse 11/10/2016

Summary

• Application: Western Blot
• Sample Tested: mouse beta cell line
• Species: Mouse

FAQs for NFATC1/NFAT2 Antibody (NB300-620). (Showing 1 - 1 of 1 FAQs).

1. You list ChIP as an application for this ab (NB300-620). Do you have any data to support it? Which species?
   • The collaborator we use to produce this monoclonal antibody has validated its use in ChIP via several publications. We do not list them on our datasheets as the products were not obtained directly through us. I am sorry for any confusion. I can provide the listings for you, please see the following links (Human: PMID 15347678 and PMID 19564342), (Mouse: PMID 12065418, PMID 15229217 and PMID 22490438), (Rat: PMID 18667424 and PMID 22566696). As ChIP is listed as a verified application on our datasheet, we will fully guarantee its function for this antibody.

Bioinformatics

• Gene Symbol: NFATC1
• Entrez:
  • 4772(Human)
  • 18018(Mouse)
  • 100361818(Rat)
• Uniprot:
  • O95644()