p62/SQSTM1 Antibody (2C11) - Azide and BSA Free

Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - WB analysis of recombinant p62 protein (immunogen, 74.51 KDa) using SQSTM1/p62 antibody (clone 2C11).

Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - WB analysis of recombinant p62 protein (immunogen, 74.51 KDa) using SQSTM1/p62 antibody (clone 2C11).

Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - WB analysis of p62 in p62 WT and KO mouse hepatocytes and HepG2 cell lysate using anti-p62/SQSTM1 antibody clone 2C11. Sample lane1: p62 +/- mouse hepatocytes, lane 2: p62 -/- mouse hepatocytes, lane3: Wt mouse hepatocytes, lane 4: HepG2 cells. This image is from a verified customer product review.

Immunocytochemistry/Immunofluorescence: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - ICC-IF analysis of SQSTM1 protein in HeLa cell using SQSTM1/p62 antibody (clone 2C11) at 10 ug/ml concentration.

Immunocytochemistry/Immunofluorescence: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Analysis of p62 in SH-SY5Y cells using anti-p62/SQSTM1 antibody. Red - p62 puncta; Blue - nuclear DAPI; Green - Cytoskeleton. Image from verified customer review.

Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Disruption of the VANGL2-p62/SQSTM1 interaction in breast cancer cells. Soft agar colony formation of T47D cells overexpressing GFP, GFP-VANGL2 and GFP-p62/SQSTM1 (right). Protein expression was revealed with anti-p62/SQSTM1, anti-VANGL2 and anti-GFP antibodies by western blot analysis (right). In anti-GFP blot, the arrowhead indicates position of co-migrating GFP-VANGL2 and GFP-p62/SQSTM1 and the asterisk pinpoints GFP alone. Error bars represent mean+/-s.d. (n=3). Image collected and cropped by CiteAb from the following publication (//www.nature.com/doifinder/10.1038/ncomms10318), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Analysis of p62/SQSTM1 antibody on HeLa cells. Image from verified customer review.

Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Confirmation of loss of hepatic ATG7 protein and a phenotype of hepatic autophagy inactivation. Fourth ug of liver protein were loaded on 4-20% gradient Tris-Glycine gels and subsequently immunoblotted with antibodies against ATG7, p62 and LC3. Blots were visualized with G:BOX Chemi XRQ (SynGene). Representative immunoblots are shown for WT and KO mice. Full-length immunoblots are presented in Supplementary Figure S1. ATG7: autophagy-related protein 7; p62: sequestosome 1; LC3: microtubule-associated protein 1 light chain 3; WT: Mx1-Cre-Atg7F/F mouse; KO: Mx1-Cre+Atg7F/F mouse. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-14405-w), licensed under a CC-BY license.

ELISA: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Detection limit for recombinant GST tagged SQSTM1 is approximately 0.03ng/ml as a capture antibody.

Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Activation of multiple signaling pathways by dandelion root extractA. Western blots of proteins involved in programmed cell death & cell survival & inflammation B. Densitometry quantification of western blot analysis from three independent experiments. *P<0.05, **P< 0.001. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.11485), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Elevated levels of additional proteins & GM3 ganglioside in the MEC of MPS IIIB brain.Staining performed with antibodies to the indicated substances was observed in the MEC region of 3 month-old MPS IIIB mice (for total ubiquitin & polyubiquitin) & 6 months for all others. Staining was not seen in the MEC region of age-matched control mice (Naglu +/−) nor in the LEC region of MPS IIIB mice (the latter not shown). Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0027461), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Confirmation of loss of hepatic ATG7 protein & a phenotype of hepatic autophagy inactivation. Fourty µg of liver protein were loaded on 4–20% gradient Tris-Glycine gels & subsequently immunoblotted with antibodies against ATG7, p62 & LC3. Blots were visualised with G:BOX Chemi XRQ (SynGene). Representative immunoblots are shown for WT & KO mice. Full-length immunoblots are presented in Supplementary Figure S1. ATG7: autophagy-related protein 7; p62: sequestosome 1; LC3: microtubule-associated protein 1 light chain 3; WT: Mx1-Cre−Atg7F/F mouse; KO: Mx1-Cre+Atg7F/F mouse. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29074879), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - The content of mitochondrial p62, OPTN, & VCP in spinal cord of SOD1‐G93A mice is decreased by Parkin knockoutImmunostaining of anterior horn of lumbar spinal cord for ChAT (in green) & p62 (in red) at 130 days. Scale bar, 10 μm.Quantification of the percentage of ChAT‐positive motor neurons (MN) containing p62‐positive inclusions at 130 days. PKO/G93A MN have less SOD1 aggregates than G93A, while Non Tg & PKO MN have none. Data were collected from n = 4 (two males & two females) mice for Non Tg & PKO & n = 6 (three males & three females) for G93A & PKO/G93A. Number of images recorded for each genotype: n = 9 for Non Tg & PKO, n = 16 for G93A, & n = 12 for PKO/G93A. Results are expressed as mean ± SEM; *P = 0.032, by unpaired one‐tailed Student's t‐test.Representative Western blots of OPTN, p62, & VCP in spinal cord mitochondrial fractions at 130 days. Protein levels are normalized by Complex V. The quantifications in panels (D–F) show that mitochondria of PKO/G93A mice have less mitophagy adaptor proteins than G93A relative to Complex V.Quantification of OPTN relative to Complex V at 130 days. Results are expressed as mean ± SEM & as percent of Non Tg; n = 8 (four males & four females); *P = 0.039 by paired Student's t‐test.Quantification of p62, relative to Complex V at 130 days. Results are expressed as mean ± SEM & as percent of Non Tg; n = 8 (four males & four females); **P = 0.0029 by paired Student's t‐test.VCP was quantified using Complex V as loading reference at 130 days. Results are expressed as mean ± SEM & as percent of Non Tg; n = 8 (four males & four females); *P = 0.044 by paired Student's t‐test.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30126943), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - miR-29a downregulates TFEB & ATG9A to inhibit autophagyA. Schematic representation of the miR-29 family members & 3′-UTR binding sites of miR-29 targets as well as mutated binding sites used in Luciferase Assays: Transcription Factor EB (TFEB) & Autophagy-related protein 9A (ATG9A). All three miR-29 family members (miR-29a, miR-29b, & miR-29c) have identical seed sequences. Conserved miR-29 binding sites in the 3′-UTR of mRNA transcripts encoding ATG9A & TFEB are depicted in bold. B. 10ug of total protein cell lysates from Panc-1 transfected with CTRL or miR-29a mimics were subjected to western blot analysis for TFEB, ATG9A, & GAPDH. Relative quantification of band intensities normalized to GAPDH are shown below respective blots. C. Relative firefly luciferase activity from TFEB & ATG9A 3′ UTR wild type (WT) & mutant (mut) reporter constructs following co-transfection into Panc-1 cells with control or miR-29a mimics. All readouts were normalized to renilla luciferase activity for each well. Average relative luminesce normalized to respective controls is presented (n=6) ± S.E.M. D. 5ug of total protein cell lysates from Panc-1 cells were transfected with CTRL, miR-29a mimics, siCTRL, siTFEB, or siATG9A. 24 hours post-transfection, total protein was harvested & subjected to western blot analysis for p62 & LC3B, & GAPDH was used as loading control. Quantification of band intensities normalized to GAPDH & relative to control are shown below respective blots. All experiments were repeated 3 times & representative data is presented. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.11928), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Effects of autophagy inhibitors in combination with IFN-gamma on expression of PD-L1 in gastric cancer cell lines. a The effect of chloroquine (CQ) or 3-MA on expression of PD-L1 with or without INF-gamma for 24 h was determined by flow cytometry assays. In AGS & NCI-n87 cells, MFI as the indication of PD-L1 expression level can be further increased by the treatment of INF-gamma . b Levels of LC3B-I/II, p62/SQSTM1 & PD-L1 were determined by Western blots in AGS & NCI-n87 cells treated by CQ, 3-MA, bafilomycin A1 (Baf) or rapamycin (Rap) for 24 h. c Positive staining of PD-L1 (red) & LC3 positive puncta (green) was determined by immunofluorescence in AGS & NCI-n87 cells treated by autophagy inhibitors & activator as in (b). d Rapamycin decreased the levels of PD-L1 protein in AGS & NCI-n87 cells as shown by flow cytometry. Results were averaged & blots were representative of 4 independent experiments, *p < 0.05, **p < 0.01 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30925913), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - The content of mitochondrial p62, OPTN, & VCP in spinal cord of SOD1‐G93A mice is decreased by Parkin knockoutImmunostaining of anterior horn of lumbar spinal cord for ChAT (in green) & p62 (in red) at 130 days. Scale bar, 10 μm.Quantification of the percentage of ChAT‐positive motor neurons (MN) containing p62‐positive inclusions at 130 days. PKO/G93A MN have less SOD1 aggregates than G93A, while Non Tg & PKO MN have none. Data were collected from n = 4 (two males & two females) mice for Non Tg & PKO & n = 6 (three males & three females) for G93A & PKO/G93A. Number of images recorded for each genotype: n = 9 for Non Tg & PKO, n = 16 for G93A, & n = 12 for PKO/G93A. Results are expressed as mean ± SEM; *P = 0.032, by unpaired one‐tailed Student's t‐test.Representative Western blots of OPTN, p62, & VCP in spinal cord mitochondrial fractions at 130 days. Protein levels are normalized by Complex V. The quantifications in panels (D–F) show that mitochondria of PKO/G93A mice have less mitophagy adaptor proteins than G93A relative to Complex V.Quantification of OPTN relative to Complex V at 130 days. Results are expressed as mean ± SEM & as percent of Non Tg; n = 8 (four males & four females); *P = 0.039 by paired Student's t‐test.Quantification of p62, relative to Complex V at 130 days. Results are expressed as mean ± SEM & as percent of Non Tg; n = 8 (four males & four females); **P = 0.0029 by paired Student's t‐test.VCP was quantified using Complex V as loading reference at 130 days. Results are expressed as mean ± SEM & as percent of Non Tg; n = 8 (four males & four females); *P = 0.044 by paired Student's t‐test.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30126943), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - G2019S LRRK2 hippocampal neurons do not have elevated alpha -synuclein pathology 14 days post-transduction. a Primary hippocampal neurons from NTG or G2019S pups were transduced with 2.5 μg/mL alpha -synuclein PFFs & allowed to age a further 14 days prior to sequential detergent fractionation. TX-100-insoluble alpha -synuclein & p62 are similar in both neuron types. b Quantification of soluble proteins shows ~ 25-fold elevation in the expression of LRRK2 & a commensurate ~ 50-fold elevation in pS395 LRRK2, indicative of the elevated LRRK2 kinase activity associated with the G2019S mutation. Soluble alpha -synuclein levels were equivalent between the cultures. c No significant differences were found between the genotypes in insoluble proteins by an unpaired t-test with Welch’s correction. (N = 3 biological replicates for each protein). Means + s.e.m.; **P < 0.01; *P < 0.05 by an unpaired t-test with Welch’s correction for unequal variances. All values are normalized to NTG neurons treated with alpha -synuclein PFFs & DMSO Image collected & cropped by CiteAb from the following publication (//actaneurocomms.biomedcentral.com/articles/10.1186/s40478-018-0550-0), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - LRRK2 inhibition does not reduce insoluble alpha -synuclein in wildtype hippocampal neurons. a Primary hippocampal neurons from CD1 pups were treated with 30 nM LRRK2 inhibitors PF-475, PF-360 or DMSO as a vehicle control, then transduced with 2.5 μg/mL alpha -synuclein PFFs & allowed to age a further 14 days prior to sequential detergent fractionation. TX-100-insoluble alpha -synuclein & p62 are similar in both LRRK2 inhibitor treated & untreated neurons. b Quantification of soluble proteins show some reduction of LRRK2 protein levels PF-475 treatment & ~ 75% inhibition of LRRK2 activity (as assayed by pS935 LRRK2) by both PF-475 & PF-360. c Insoluble pS129 alpha -synuclein was slightly, but significantly elevated by PF-360 treatment, while alpha -synuclein & p62 were unchanged by one-way ANOVA. (N = 5 biological replicates for each protein). Means + s.e.m.; *P < 0.05, ****p < 0.0001 by one-way ANOVA with Dunnett’s multiple comparison test. All values are normalized first to GAPDH, as a loading control, then to DMSO-treated neurons Image collected & cropped by CiteAb from the following publication (//actaneurocomms.biomedcentral.com/articles/10.1186/s40478-018-0550-0), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Colocalization of VANGL2 & p62/SQSTM1 in late endosomes.(a) Immunofluorescence staining of SKBR7 cells showed colocalization of endogenous VANGL2 (green) & p62/SQSTM1 (red) in discrete cytoplasmic puncta. Scale bar, 10 μm. The mean Pearson correlation for VANGL2 & p62/SQSTM1 is 0.62, calculated using the Image J software for ∼15 cells per field of view & from 10 images. (b) Partial colocalization of VANGL2 & p62/SQSTM1 in late endosomes of SKBR7 cells stained w/ the LAMP1 marker. Scale bar, 20 μm. (c) SKBR7 ells cultured in a nutrient-deprived medium & treated w/ 100 nM bafilomycin A1 (6 h) before fixation. Double labelling against VANGL2 (arrowheads) & p62/SQSTM1 (arrows), as described in Methods, showed accumulations of both markers in vesicular structures probably resembling endosomes/amphisomes. Scale bar, 200 nm. (d) IMCD3 cells treated w/ PBS or EGTA (5 mM) for 30 min. Immunofluorescence & confocal analysis performed using the indicated antibodies. Scale bar, 10 μm. Inserts show colocalized VANGL2 & p62/SQSTM1. (e) The VANGL2–p62/SQSTM1 complex recovered in confluent SKBR7 or IMCD3 cells w/ 2G4 mAb (IP VANGL2) but not a control antibody (IP IgG2a ctrl) as seen using western blot analysis w/ the indicated antibodies. The complex more abundant in cancer cells (SKBR7) than in polarized cells (IMCD3). Note that human (SKBR7 cells) & murine (IMCD3 cells) p62/SQSTM1 run at different molecular weights. (f) Lysates of confluent IMCD3 cells treated for 30 min w/ PBS or EGTA subjected to 2G4 mAb immunoprecipitation (IP VANGL2). Immunoprecipitated proteins probed by western blot analysis w/ the indicated antibodies. Increased amounts of VANGL2–p62/SQSTM1 complexes recovered after EGTA treatment. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms10318), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Colocalization of VANGL2 & p62/SQSTM1 in late endosomes.(a) Immunofluorescence staining of SKBR7 cells showed colocalization of endogenous VANGL2 (green) & p62/SQSTM1 (red) in discrete cytoplasmic puncta. Scale bar, 10 μm. The mean Pearson correlation for VANGL2 & p62/SQSTM1 is 0.62, calculated using the Image J software for ∼15 cells per field of view & from 10 images. (b) Partial colocalization of VANGL2 & p62/SQSTM1 in late endosomes of SKBR7 cells stained w/ the LAMP1 marker. Scale bar, 20 μm. (c) SKBR7 ells cultured in a nutrient-deprived medium & treated w/ 100 nM bafilomycin A1 (6 h) before fixation. Double labelling against VANGL2 (arrowheads) & p62/SQSTM1 (arrows), as described in Methods, showed accumulations of both markers in vesicular structures probably resembling endosomes/amphisomes. Scale bar, 200 nm. (d) IMCD3 cells treated w/ PBS or EGTA (5 mM) for 30 min. Immunofluorescence & confocal analysis performed using the indicated antibodies. Scale bar, 10 μm. Inserts show colocalized VANGL2 & p62/SQSTM1. (e) The VANGL2–p62/SQSTM1 complex recovered in confluent SKBR7 or IMCD3 cells w/ 2G4 mAb (IP VANGL2) but not a control antibody (IP IgG2a ctrl) as seen using western blot analysis w/ the indicated antibodies. The complex more abundant in cancer cells (SKBR7) than in polarized cells (IMCD3). Note that human (SKBR7 cells) & murine (IMCD3 cells) p62/SQSTM1 run at different molecular weights. (f) Lysates of confluent IMCD3 cells treated for 30 min w/ PBS or EGTA subjected to 2G4 mAb immunoprecipitation (IP VANGL2). Immunoprecipitated proteins probed by western blot analysis w/ the indicated antibodies. Increased amounts of VANGL2–p62/SQSTM1 complexes recovered after EGTA treatment. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms10318), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Parkin protein levels are unaffected in liver while a small decrease is measured in G93A cerebellumRepresentative Western blot for Parkin & p62 in cerebellum (Cb) homogenates at 130 days.Quantification indicates that Parkin is decreased in G93A mice relative to Non Tg at 130 days. Protein levels were normalized by beta ‐actin. Results are expressed as mean ± SEM & percent of Non Tg; n = 8 (four males & four females) mice per group. *P = 0.039 by paired Wilcoxon's test.Quantification of p62 in the homogenates indicates that p62 is increased in PKO & PKO/G93A mice, independent of SOD1‐G93A expression, at 130 days of age. beta ‐actin was used for normalization. Results are expressed as mean ± SEM & percent of Non Tg; n = 8 (four males & four females) mice per group. No statistically significant differences were found between Non Tg & G93A by paired Friedman's test with Dunn's correction (P = 0.99). ***P = 0.0001 by paired Student's t‐test (G93A vs. PKO/G93A).Representative Western blot of Parkin & p62 in liver homogenates at 130 days.Parkin protein levels were quantified at 130 days. beta ‐actin was used for normalization. Results are expressed as mean ± SEM & percent of Non Tg; n = 8 (four males & four females) mice per group. No statistically significant differences were found between Non Tg & G93A (P = 0.546 by paired Wilcoxon's test).Quantification of p62 protein levels in liver at 130 days. Results are expressed as mean ± SEM & percent of Non Tg; n = 8 (four males & four females) mice per group. No statistically significant differences were found between Non Tg & G93A (P = 0.546 by paired Wilcoxon's test). No statistically significant differences were found among the other groups.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30126943), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Parkin knockout mitigates the accumulation of mitophagy adaptors in G93A mitochondria at disease end stageA, BRepresentative Western blots of p62 (A), OPTN, & VCP (B) in spinal cord mitochondria from end‐stage mice.Cp62 quantification, using Complex V as normalizer, shows a strong accumulation of p62 in SOD1‐G93A mitochondria at end stage. Results are expressed as mean ± SEM & as percent of Non Tg; n = 8 (four males & four females) mice per group. No statistically significant differences were found between G93A & PKO/G93A (P = 0.078 by paired Wilcoxon's test); ***P = 0.0007 (Non Tg vs. G93A) & *P = 0.037 (PKO & PKO/G93A) both by paired Friedman's test with Dunn's correction.DQuantification of OPTN accumulation in end‐stage mitochondria, with Complex V as protein loading control. Results are expressed as mean ± SEM & as percent of Non Tg; n = 8 (four males & four females) mice per group. **P = 0.0078 (for G93A & PKO/G93A) by paired Wilcoxon's test; ***P = 0.0003 (Non Tg vs. G93A) by paired Friedman's test with Dunn's correction. No other statistically significant differences were found.EQuantification of VCP in mitochondria at disease end stage. Complex V was used as normalizer. Results are expressed as mean ± SEM & as percent of Non Tg; n = 8 (four males & four females) mice per group. *P = 0.039 (for G93A & PKO/G93A) by paired Wilcoxon's test; ***P = 0.0007 (Non Tg vs. G93A) & *P = 0.037 (PKO & PKO/G93A) both by paired Friedman's test with Dunn's correction.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30126943), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Bv, Ha, Rb
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clone: 2C11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

p62/SQSTM1 Antibody (2C11) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: SQSTM1 (AAH03139.1, 1 a.a. ~ 440 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASLTVKAYLLGKEDAAREIRRFSFCCSPEPEAEAEAAAGPGPCERLLSRVAALFPALRPGGFQAHYRDEDGDLVAFSSDEELTMAMSYVKDDIFRIYIKEKKECRRDHRPPCAQEAPRNMVHPNVICDGCNGPVVGTRYKCSVCPDYDLCSVCEGKGLHRGHTKLAFPSPFGHLSEGFSHSRWLRKVKHGHFGWPGWEMGPPGNWSPRPPRAGEARPGPTAESASGPSEDPSVNFLKNVGESVAAALSPLGIEVDIDVEHGGKRSRLTPVSPESSSTEEKSSSQPSSCCSDPSKPGGNVEGATQSLAEQMRKIALESEGRPEEQMESDNCSGGDDDWTHLSSKEVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKHPPPL
• Localization: Cytoplasmic
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SQSTM1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 10ug/mL
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:1000
  • Western Blot 1:100-1:2000
• Application Notes: Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 23569437).
• Theoretical MW: 47.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 24352657). Human reactivity reported in scientific literature (PMID: 26571504). Rabbit reactivity reported in scientific literature (PMID: 23569437). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for p62/SQSTM1 Antibody (2C11) - Azide and BSA Free

• A170
• Autophagy Receptor P62
• DMRV
• EBI3-associated protein of 60 kDa
• EBI3-associated protein p60
• EBIAP
• FTDALS3
• NADGP
• ORCA
• OSIL
• oxidative stress induced like
• p60PDB3
• p62
• p62B
• Paget disease of bone 3
• PDB3
• phosphotyrosine independent ligand for the Lck SH2 domain p62
• Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa
• Sequestosome 1
• sequestosome-1
• SQSTM1
• Ubiquitin-binding protein p62
• ZIP3

Background

p62/SQSTM1 (ubiquitin-binding protein p62/Sequestrosome-1) is an intracellular protein (theoretical molecular weight 47.7 kDa) which localizes to the cytoplasm, nucleus, autophagosomes and lysosomes in all tissues (1). Structurally, p62/SQSTM1 consists of multiple domains (e.g., Phox1 and Bem1p-PB1, zinc finger-ZZ, TRAF6 binding domain-TB, Keap1-interacting region-KIR, LC3 interacting region-LIR and ubiquitin-associated domain-UBA) for interaction with various protein targets. Additional domains include nuclear export (NES) and nuclear localization signals (NLS1 and NLS2). p62/SQSTM1 is a multifunctional scaffold protein which forms oligomers with itself or with other proteins through interactions with PB1 domains.

Abnormal function of p62/SQSTM1 is associated with a range of disease states such as neurodegeneration, cancer, and metabolic disorders (2). Mutations in the p62/SQSTM1 sequence have been linked to Paget's disease of the bone, amyotrophic lateral sclerosis, and frontotemporal lobar degeneration. In Parkinson's disease, p62/SQSTM1 has been linked to microglia activation and subsequent neuroinflammation (3). Functionally, p62/SQSTM1 is involved in a broad range of cellular processes such as amino acid sensing by interaction with mTORC1, oxidative stress response through interaction with Keap1, and targeting cargo for autophagy by interacting with ubiquitin labeled proteins (1).

To induce selective autophagy, p62/SQSTM1 forms long oligomers or helical filaments which interact with LC3 and ubiquitin labeled proteins and lead to the initiation of the autophagosome formation (2). p62/SQSTM1 is not only a selective autophagy receptor but also an autophagy substrate, as its engulfed by the autophagosome and degraded by the autophagolysosome. Monitoring LC3 levels is the standard for assessing autophagic flux, however monitoring p62/SQSTM1 levels by Western blot in the presence and absence of autophagy inhibitors (e.g., Chloroquine) is also a common practice (4). Besides its activity as a selective autophagy receptor, p62/SQSTM1 also plays a role as an adaptor in signaling cascades leading to NFkB activation downstream of TNF-R, IL-1 beta R, TrkA and p75NTR. Briefly, for NFkB signaling downstream of the TNF-R activation, p62/SQSTM1 engages RIP1 kinase and PKC iota/lambda through the ZZ and PB1 domains, respectively (5).

References

1.Katsuragi, Y., Ichimura, Y., & Komatsu, M. (2015). P62/SQSTM1 functions as a signaling hub and an autophagy adaptor. FEBS Journal. https://doi.org/10.1111/febs.13540

2. Sanchez-Martin, P., & Komatsu, M. (2018). p62/SQSTM1 - Steering the cell through health and disease. Journal of Cell Science. https://doi.org/10.1242/jcs.222836

3. Yao, L., Zhu, Z., Wu, J., Zhang, Y., Zhang, H., Sun, X., ... Lu, G. (2019). MicroRNA-124 regulates the expression of p62/p38 and promotes autophagy in the inflammatory pathogenesis of Parkinson's disease. The FASEB Journal. https://doi.org/10.1096/fj.201900363r

4 Klionsky, D. J., Abdelmohsen, K., Abe, A., Abedin, M. J., Abeliovich, H., Arozena, A. A., ... Zughaier, S. M. (2016). Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy. https://doi.org/10.1080/15548627.2015.1100356

5. Bitto, A., Lerner, C. A., Nacarelli, T., Crowe, E., Torres, C., & Sell, C. (2014). p62/SQSTM1 at the interface of aging, autophagy, and disease. Age. https://doi.org/10.1007/s11357-014-9626-3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for p62/SQSTM1 Antibody (H00008878-M01)(433)

Publications using H00008878-M01

• Shih-Hong Khoo, Pei-Ru Wu, Kun-Tu Yeh, Shih-Lan Hsu, Chi-Hao Wu Biological and clinical significance of the AGE-RAGE axis in the aggressiveness and prognosis of prostate cancer Journal of Food and Drug Analysis 2023-01-01 [PMID: 38526823]
• Williams JA, Ni HM, Ding Y et al. Parkin Regulates Mitophagy and Mitochondrial Function to Protect Against Alcohol-induced Liver Injury and Steatosis in Mice. American Journal of Physilogy Jul 9 2015
• Ainhoa Martínez-Pizarro, Sara Picó, Arístides López-Márquez, Claudia Rodriguez-López, Elena Montalvo, Mar Alvarez, Margarita Castro, Santiago Ramón-Maiques, Belén Pérez, José J Lucas, Eva Richard, Lourdes R Desviat PAH deficient pathology in humanized c.1066-11G>A phenylketonuria mice Human Molecular Genetics 2024-06-15 [PMID: 38520741]
• Karanasios E, Ktistakis NT. Studying Autophagy: List of Useful Antibodies Produced via a Community Effort. Autophagy at the Cell, Tissue and Organismal Level. 2016-04-24
• Ji Y, Jeon YG, Lee WT et Al. PKA regulates autophagy through lipolysis during fasting Mol Cells 2024-11-13 [PMID: 39547583]
• Jia N, Ganesan D, Guan H et Al. Mitochondrial bioenergetics stimulates autophagy for pathological MAPT/Tau clearance in tauopathy neurons Autophagy 2024-09-03 [PMID: 39171695]
• Ross R, Guo Y, Walker RN et al. Biomechanical Activation of Keloid Fibroblasts Promotes Lysosomal Remodeling and Exocytosis. The Journal of investigative dermatology 2024-05-17 [PMID: 38763173]
• Onal G, Yalç?n-Çakmakl? G, Özçelik CE, Boussaad I et Al. Variant-specific effects of GBA1 mutations on dopaminergic neuron proteostasis J Neurochem 2024-04-20 [PMID: 38641924]
• Shannon Nicolson, Jantina A. Manning, Yoon Lim, Xin Jiang, Erica Kolze, Sonia Dayan, Ruchi Umargamwala, Tianqi Xu, Jarrod J. Sandow, Andrew I. Webb, Sharad Kumar, Donna Denton The Drosophila ZNRF1/2 homologue, detour, interacts with HOPS complex and regulates autophagy Communications Biology 2024-02-15 [PMID: 38360932]
• Siddique T, Chen W, Deng H et al. Molecular Targets for ALS and Related Disorders. United States Patent Application Mar 17 2016
• Ko SH, Choi JH, Kim JM. Bacteroides fragilis Enterotoxin Induces Autophagy through an AMPK and FoxO3-Pathway, Leading to the Inhibition of Apoptosis in Intestinal Epithelial Cells Toxins (Basel) 2023-09-03 [PMID: 37755970]
• Chedrewih M, Thiery V, Gauthier A et Al. Incorporation of antimony-bearing mining wastes into clinker Portland raw feed: The difficulty of Sb analysis in calcium silicates J Microsc 2023-09-14 [PMID: 37706543]
• Brochard L Fatty acid-induced stress signalling in adipocytes Thesis 1905-07-11 (WB, Human)
• Xiaosen Ouyang, Willayat Y. Wani, Gloria A. Benavides, Matthew J. Redmann, Hai Vo, Thomas van Groen, Victor M. Darley-Usmar, Jianhua Zhang Cathepsin D overexpression in the nervous system rescues lethality and A beta 42 accumulation of cathepsin D systemic knockout in vivo Acta Pharmaceutica Sinica. B 2023-07-20 [PMID: 37799377]
• Lee B, Kim YH, Lee W et al. USP13 deubiquitinates p62/SQSTM1 to induce autophagy and Nrf2 release for activating antioxidant response genes Free radical biology & medicine 2023-11-01 [PMID: 37776917] (BA, Human)
• Stuard Sambhariya W, Trautmann IJ, Robertson DM Insulin-like growth factor binding protein-3 mediates hyperosmolar stress-induced mitophagy through the mechanistic target of rapamycin The Journal of biological chemistry 2023-09-08 [PMID: 37690686] (WB, Human)
• Kim J, Jeon T, Baek K et al. Effects of 10-Week Treadmill Exercise on the Improvement of Obesity-Induced Autophagy Dysregulation in Arterial Endothelial Cells of Mice Exerc Sci 2019-08-31 (IB, Mouse)
• Siddique T, Chen W, Deng H et al. Molecular Targets for ALS and Related Disorders. United States Patent Application. 2016-01-01
• Zhang X, Shan G, Li N et al. An autophagy-inducing stapled peptide induces mitochondria dysfunction and triggers autotic cell death in triple-negative breast cancer Cell Death Discovery 2023-08-19 [PMID: 37598181]
• Leduc-Gaudet J, Miguez K, Cefis M et al. Autophagy ablation in skeletal muscles worsens sepsis-induced muscle wasting, impairs whole-body metabolism, and decreases survival. iScience 2023-07-01 [PMID: 37588163] (WB, Mouse)
• Tamargo-Gómez I, Martínez-García GG, Suárez MF et al. Analysis of ATG4C function in vivo Autophagy 2023-07-17 [PMID: 37459465]
• McKinney JR, Seferovic MD, Major AM et al. Placental Autophagy and Viral Replication Co-localize in Human and Non-human Primate Placentae Following Zika Virus Infection: Implications for Therapeutic Interventions Frontiers in Virology 2021-09-17 [PMID: 37431450]
• Calcagni' A, Staiano L, Zampelli N et al. Loss of the batten disease protein CLN3 leads to mis-trafficking of M6PR and defective autophagic-lysosomal reformation Nature communications 2023-07-03 [PMID: 37400440] (WB)
• Tejwani L, Jung Y, Kokubu H et al. Reduction of nemo-like kinase increases lysosome biogenesis and ameliorates TDP-43-related neurodegeneration Journal of Clinical Investigation 2023-08-15 [PMID: 37384409]
• Iemolo A, De Risi M, Giordano N et al. Synaptic mechanisms underlying onset and progression of memory deficits caused by hippocampal and midbrain synucleinopathy NPJ Parkinson's disease 2023-06-16 [PMID: 37328503]
• Mengzhen Y, Bing H, Jiajia L et al. Coronaviral ORF6 protein mediates inter-organelle contacts and modulates host cell lipid flux for virus production. EMBO J. 2023-05-23 [PMID: 37218505]
• Jianbin W, Andreas Z, Nils B et al. Loss of function of FIP200 in human pluripotent stem cell-derived neurons leads to axonal pathology and hyperactivity. Transl Psychiatry. 2023-05-03 [PMID: 37137886]
• Lee E, Jung Y, Park Y et al. A distinct astrocyte subtype in the aging mouse brain characterized by impaired protein homeostasis Nature Aging 2022-08-01 [PMID: 37118130] (IHC-Fr, Mouse)
• S Mukhopadhy, J Encarnació, EY Lin, ASW Sohn, H Zhang, JD Mancias, AC Kimmelman Autophagy supports mitochondrial metabolism through the regulation of iron homeostasis in pancreatic cancer Science Advances, 2023-04-19;9(16):eadf9284. 2023-04-19 [PMID: 37075122]
• Tracy H, Sierra C, Alder L et al. STAT3 Drives GFAP Accumulation and Astrocyte Pathology in a Mouse Model of Alexander Disease. Cells. 2023-03-23 [PMID: 37048051]
• Xuan T, Kun C, Jiajia L et al. Coronavirus subverts ER-phagy by hijacking FAM134B and ATL3 into p62 condensates to facilitate viral replication. Cell Rep. 2023-03-10 [PMID: 36952345]
• Shahin S, Lu B, Zhou Y et al. MFN1 augmentation prevents retinal degeneration in a Charcot-Marie-Tooth type 2A mouse model iScience 2023-02-01 [PMID: 36936780] (IHC, WB, Mouse)
• Vidyadhara DJ, Somayaji M, Wade N et al. Dopamine transporter and synaptic vesicle sorting defects underlie auxilin-associated Parkinson's disease Cell reports 2023-03-28 [PMID: 36920906] (IHC-Fr, WB, Mouse)
  
  Details:
  Dilution used in WB 1:1,000, IHC-Fr 1:200
• Francois G, Eric B, William R et al. TNIP1 inhibits selective autophagy via bipartite interaction with LC3/GABARAP and TAX1BP1. Mol Cell. 2023-03-09 [PMID: 36898370]
• Julien B, Leanne H, Sofia H et al. Modifying Dietary Protein Impacts mTOR Signaling and Brain Deposition of Amyloid ? in a Knock-In Mouse Model of Alzheimer Disease. J Nutr. 2023-03-03 [PMID: 36870538]
• Southwell N, Zhao D, Sayles NM et al. High fat diet ameliorates the mitochondrial cardiomyopathy of CHCHD10 mutant mice bioRxiv 2023-02-22 [PMID: 36865125]
• MJ Corenblum, A McRobbie-J, E Carruth, K Bernard, M Luo, LJ Mandarino, S Peterson, D Billheimer, T Maley, ED Eggers, L Madhavan Parallel Neurodegenerative Phenotypes in Sporadic Parkinson&#039;s Disease Fibroblasts and Midbrain Dopamine Neurons bioRxiv : the preprint server for biology, 2023-02-12;0(0):. 2023-02-12 [PMID: 36798207]
• Hui Q, Xiaojuan C, Shaogui W et al. Loss of SQSTM1/p62 Induces Obesity and Exacerbates Alcohol-induced Liver Injury in Aged Mice. Cell Mol Gastroenterol Hepatol. 2023-02-06 [PMID: 36754207]
• Pablo S, Franziska K, Ludovico A et al. ULK1-mediated phosphorylation regulates the conserved role of YKT6 in autophagy. J Cell Sci. 2023-02-09 [PMID: 36644903]
• Yi L, Michael T, Liang H et al. In-Cell Chemical Crosslinking Identifies Hotspots for SQSTM-1/p62-I?B? Interaction That Underscore a Critical Role of p62 in Limiting NF-?B Activation Through I?B? Stabilization. Mol Cell Proteomics. 2023-01-10 [PMID: 36634736]
• Guo L, Reed K, Carter A et al. Sleep-Disturbance-Induced Microglial Activation Involves CRH-Mediated Galectin 3 and Autophagy Dysregulation Cells 2022-12-30 [PMID: 36611953] (WB, Mouse)
• Charlotte G, Alix S, Marie B et al. Tamoxifen improves muscle structure and function of Bin1- and Dnm2-related centronuclear myopathies. Brain. 2022-12-23 [PMID: 36562127]
• Miller K, Wagner MA, Jassey A, Jackson WT SNAP23 is essential for germination of EV-D68 replication organelles Virology 2022-12-12 [PMID: 36527930]
• Inoue M, Noguchi S, Inoue YU et al. Distinctive chaperonopathy in skeletal muscle associated with the dominant variant in DNAJB4 Acta neuropathologica 2023-02-01 [PMID: 36512060]
• Yunash M, Raghbendra D, Jinbae S et al. Intracellular cholesterol transport inhibition Impairs autophagy flux by decreasing autophagosome-lysosome fusion. Cell Commun Signal. 2022-11-25 [PMID: 36434621]
• Caitlin L, Erica M, Jeanelle A et al. Protocol for the Systematic Fixation, Circuit-Based Sampling, and Qualitative and Quantitative Neuropathological Analysis of Human Brain Tissue. Methods Mol Biol. 2022-11-19 [PMID: 36399262]
• Jingyi C, Xiaozhe Z, Shan G et al. A Beclin 1-targeting stapled peptide synergizes with erlotinib to potently inhibit proliferation of non-small-cell lung cancer cells. Biochem Biophys Res Commun. 2022-10-20 [PMID: 36332474]
• Qu W, Jeong A, Zhong R et al. Deletion of Small GTPase H-Ras Rescues Memory Deficits and Reduces Amyloid Plaque-Associated Dendritic Spine Loss in Transgenic Alzheimer's Mice Molecular neurobiology 2022-10-26 [PMID: 36287323]
• Sang-Won P, Pureum J, Akinori Y et al. Development of new tools to study membrane-anchored mammalian Atg8 proteins. Autophagy. 2022-10-17 [PMID: 36250672]
• Kim YS, Ko B, Kim DJ et al. Induction of the hepatic aryl hydrocarbon receptor by alcohol dysregulates autophagy and phospholipid metabolism via PPP2R2D Nature communications 2022-10-14 [PMID: 36241614]
• Alba N, Irene F, Jordi R et al. Lysosomal lipid alterations caused by glucocerebrosidase deficiency promote lysosomal dysfunction, chaperone-mediated-autophagy deficiency, and alpha-synuclein pathology. NPJ Parkinsons Dis. 2022-10-06 [PMID: 36202848]
• Sofia A, Pablo M, Steffen H et al. OMA1-mediated integrated stress response protects against ferroptosis in mitochondrial cardiomyopathy. Cell Metab. 2022-09-15 [PMID: 36113464]
• Baytas O, Kauer J, Morrow E Loss of mitochondrial enzyme GPT2 causes early neurodegeneration in locus coeruleus Neurobiology of Disease 2022-07-01 [PMID: 35908744] (IHC-Fr, Mouse)
• Charles T, Gianni G Prolonged proteasome inhibition antagonizes TGF?1-dependent signalling by promoting the lysosomal-targeting of TGF? receptors. Cell Signal. 2022-07-25 [PMID: 35901932]
• Akwa Y, Di Malta C, Zallo F et al. Stimulation of synaptic activity promotes TFEB-mediated clearance of pathological MAPT/Tau in cellular and mouse models of tauopathies Autophagy 2022-07-22 [PMID: 35867714]
• Silva-Rojas R, P�rez-Gu�rdia L, Lafabrie E et al. Silencing of the Ca(2+) Channel ORAI1 Improves the Multi-Systemic Phenotype of Tubular Aggregate Myopathy (TAM) and Stormorken Syndrome (STRMK) in Mice International Journal of Molecular Sciences 2022-06-23 [PMID: 35805973]
• Bojana K, Tihana B, Alexander S et al. Ubiquitin profiling of lysophagy identifies actin stabilizer CNN2 as a target of VCP/p97 and uncovers a link to HSPB1. Mol Cell. 2022-06-28 [PMID: 35793674]
• Yang S, Liu T, Hu C et al. Ginsenoside Compound K Protects against Obesity through Pharmacological Targeting of Glucocorticoid Receptor to Activate Lipophagy and Lipid Metabolism Pharmaceutics 2022-06-02 [PMID: 35745765]
• Ki-Tae J, Seon-Hee O The role of autophagy in cadmium-induced acute toxicity in glomerular mesangial cells and tracking polyubiquitination of cytoplasmic p53 as a biomarker. Exp Mol Med. 2022-05-27 [PMID: 35624155]
• Sinem D, Yunus A, S?la T et al. A novel ATG5 interaction with Ku70 potentiates DNA repair upon genotoxic stress. Sci Rep. 2022-05-17 [PMID: 35581289]
• Chua JP, Bedi K, Paulsen MT et al. Myotubularin-related phosphatase 5 is a critical determinant of autophagy in neurons Current biology : CB 2022-05-10 [PMID: 35580604]
• Xiao X, Philip. C, Mei X et al. A racemosin B derivative, C25, suppresses breast cancer growth via lysosomal membrane permeabilization and inhibition of autophagic flux. Biochem Pharmacol. 2022-05-02 [PMID: 35513042]
• Huang H, Ouyang Q, Mei K et al. Acetylation of SCFD1 regulates SNARE complex formation and autophagosome-lysosome fusion Autophagy 2022-04-24 [PMID: 35465820] (WB, Human)
• Shahin S, Xu H, Lu B et al. AAV-CRISPR/Cas9 Gene Editing Preserves Long-Term Vision in the P23H Rat Model of Autosomal Dominant Retinitis Pigmentosa Pharmaceutics 2022-04-09 [PMID: 35456659] (IF/IHC)
• Jassey A, Wagner MA, Galitska G et al. Starvation after infection restricts enterovirus D68 replication Autophagy 2022-04-21 [PMID: 35446171]
• Del Giudice R, Imbimbo P, Pietrocola F et al. Autophagy Alteration in ApoA-I Related Systemic Amyloidosis International journal of molecular sciences 2022-03-23 [PMID: 35408859] (WB, Human)
• Xiaopeng X, Sumati H, Lyndsay Y et al. Activation of Drp1 promotes fatty acids-induced metabolic reprograming to potentiate Wnt signaling in colon cancer. Cell Death Differ. 2022-03-24 [PMID: 35332310]
• Zuleika L, Basim R, Andrew W et al. Free fatty acid treatment of mouse preimplantation embryos demonstrates contrasting effects of palmitic acid and oleic acid on autophagy. Am J Physiol Cell Physiol. 2022-03-23 [PMID: 35319901]
• Van H, Gloria B, Michelle J et al. Acute inhibition of OGA sex-dependently alters the networks associated with bioenergetics, autophagy, and neurodegeneration. Mol Brain. 2022-03-05 [PMID: 35248135]
• Vessey KA, Jobling AI, Tran MX et al. Treatments targeting autophagy ameliorate the age-related macular degeneration phenotype in mice lacking APOE (apolipoprotein E) Autophagy 2022-02-23 [PMID: 35196199]
• Da L, Jeong P, Yu L et al. PERK prevents hepatic lipotoxicity by activating the p62-ULK1 axis-mediated noncanonical KEAP1-Nrf2 pathway. Redox Biol. 2022-01-14 [PMID: 35091323]
• Yancheng T, Liming W, Tao Y et al. Synergistic effects of autophagy/mitophagy inhibitors and magnolol promote apoptosis and antitumor efficacy. Acta Pharm Sin B. 2021-06-16 [PMID: 35024319]
• Hui Q, Qingyun B, Xiao Y et al. Dual roles of p62/SQSTM1 in the injury and recovery phases of acetaminophen-induced liver injury in mice. Acta Pharm Sin B. 2021-11-16 [PMID: 35024307]
• Nah J, Shirakabe A, Mukai R et al. Ulk1-dependent alternative mitophagy plays a protective role during pressure overload in the heart Cardiovascular research 2022-01-09 [PMID: 35018428] (Western Blot)
• Vankrunkelsven W, Derde S, Gunst J et al. Obesity attenuates inflammation, protein catabolism, dyslipidaemia, and muscle weakness during sepsis, independent of leptin Journal of cachexia, sarcopenia and muscle 2022-01-07 [PMID: 34994068] (WB, Mouse)
• Quanhui T, Jie C, Gao G et al. Adenovirus vector encoding TPPII ignites HBV-specific CTL response by activating autophagy in CD8+T cell. J Viral Hepat. 2021-12-13 [PMID: 34902200]
• Sabine W, Catherine W, Ieva A et al. Inhibiting autophagy increases the efficacy of low-dose photodynamic therapy. Biochem Pharmacol. 2021-11-12 [PMID: 34780750]
• Francois-Xavier T, Benjamin L, Ari S et al. P62 accumulates through neuroanatomical circuits in response to tauopathy propagation. Acta Neuropathol Commun. 2021-11-02 [PMID: 34727983]
• Xiaojuan C, Shaogui W, Madeline H et al. Loss of Hepatic Transcription Factor EB Attenuates Alcohol-Associated Liver Carcinogenesis. Am J Pathol. 2021-10-27 [PMID: 34717896]
• Shaogui W, Xiaojuan C, Xiaoxiao J et al. Loss of acinar cell VMP1 triggers spontaneous pancreatitis in mice. Autophagy. 2021-10-28 [PMID: 34709991]
• Millarte V, Schlienger S, KAlin S, Spiess M Rabaptin5 targets autophagy to damaged endosomes and Salmonella vacuoles via FIP200 and ATG16L1 EMBO reports 2021-10-26 [PMID: 34704340]
• Peng Z, Zhao C, Du X Et al. Autophagy Induced by Palmitic Acid Regulates Neutrophil Adhesion Through the Granule-Dependent Degradation of alpha M beta 2 Integrin in Dairy Cows With Fatty Liver Frontiers in immunology 2021-10-07 [PMID: 34691032]
• Cheng Y, Kim WK, Wellman LL Et al. Short-Term Sleep Fragmentation Dysregulates Autophagy in a Brain Region-Specific Manner Life (Basel, Switzerland) 2021-10-16 [PMID: 34685469] (Mouse)
• Taneli H, Timo B, Juha K et al. Global Rhes knockout in the Q175 Huntington&#39;s disease mouse model. PLoS One. 2021-10-14 [PMID: 34648564]
• Andreas B, Laura T, William H et al. Autophagy, TERT, and mitochondrial dysfunction in hyperoxia. Am J Physiol Heart Circ Physiol. 2021-09-24 [PMID: 34559580]
• LS Alves, ARA Marques, N Padrão, FA Carvalho, J Ramalho, C Lopes, MIL Soares, CE Futter, TMVD Pinho E Me, NC Santos, OV Vieira Cholesteryl hemiazelate causes lysosome dysfunction impacting vascular smooth muscle cells homeostasis Journal of Cell Science, 2021-10-22;0(0):. 2021-10-22 [PMID: 34528688]
• Hua S, Hong-Min N, Jennifer M et al. Liver-specific deletion of mechanistic target of rapamycin does not protect against acetaminophen-induced liver injury in mice. Liver Res. 2021-03-19 [PMID: 34504721]
• Pelaz SG, Ollauri-Ib��ez C, Lillo C, Tabernero A. Impairment of Autophagic Flux Participates in the Antitumor Effects of TAT-Cx43(266-283) in Glioblastoma Stem Cells Cancers (Basel) 2021-08-24 [PMID: 34503072]
• Yin S, Shengfeng X, Natalie N et al. Rag GTPases suppress PRL-3 degradation and predict poor clinical diagnosis of cancer patients with low PRL-3 mRNA expression. Biochem Biophys Res Commun. 2021-08-31 [PMID: 34482023]
• Yamina H, Francois-Xavier M, Marine M et al. Compromised mitochondrial quality control triggers lipin1-related rhabdomyolysis. Cell Rep Med. 2021-08-17 [PMID: 34467247]
• Yueyue L, Qiaoying H, Zijian W et al. GSK-3 mediates nuclear translocation of p62/SQSTM1 in MPTP-induced mouse model of Parkinson's disease. Neurosci Lett. 2021-08-13 [PMID: 34400288]
• Silva-Rojas R, Charles A, Djeddi S Et Al. Pathophysiological Effects of Overactive STIM1 on Murine Muscle Function and Structure Cells 2021-07-08 [PMID: 34359900]
• Bellanger D, Dziagwa C, Guimaraes C et al. Adipocytes Promote Breast Cancer Cell Survival and Migration through Autophagy Activation Cancers (Basel) 2021-08-03 [PMID: 34359819]
• Dulac M, Leduc-Gaudet Jp, Cefis M Et Al. Regulation of muscle and mitochondrial health by the mitochondrial fission protein Drp1 in aged mice The Journal of physiology 2021-07-16 [PMID: 34269418]
• Hou K, Shen J, Yan J Et Al. Loss of TRIM21 alleviates cardiotoxicity by suppressing ferroptosis induced by the chemotherapeutic agent doxorubicin EBioMedicine 2021-07-01 [PMID: 34233258] (WB, IF/IHC)
• Annadurai A, Nhat N, Arjun S et al. NRF2 Loss Accentuates Parkinsonian Pathology and Behavioral Dysfunction in Human ?-Synuclein Overexpressing Mice. Aging Dis. 2021-07-01 [PMID: 34221542]
• Fontaine A, Bellanger D, Guibon R Et Al. Lipophagy and prostate cancer: association with disease aggressiveness and proximity to periprostatic adipose tissue The Journal of pathology 2021-07-05 [PMID: 34219239] (WB)
• Kiyotoshi S, Yukitoshi T, Motohiko S et al. Loss of FYCO1 leads to cataract formation. Sci Rep. 2021-07-02 [PMID: 34215815]
• Benito-Cuesta I, Ordonez-Gutierrez L, Wandosell F Trehalose Reduces the Secreted Beta-Amyloid Levels in Primary Neurons Independently of Autophagy Induction Metabolites 2021-06-26 [PMID: 34206776] (WB)
• Towers CG, Wodetzki DK, Thorburn J et al. Mitochondrial-derived vesicles compensate for loss of LC3-mediated mitophagy Developmental Cell 2021-07-01 [PMID: 34171288]
• Hao Chen, Katherine E. Williams, Elaine Y. Kwan, Mirhan Kapidzic, Kenisha A. Puckett, Rayyan K. Aburajab, Joshua F. Robinson, Susan J. Fisher Global proteomic analyses of human cytotrophoblast differentiation/invasion Development (Cambridge, England) 2021-07-01 [PMID: 34121116]
• De Risi M, Tufano M, Alvino FG et al. Altered heparan sulfate metabolism during development triggers dopamine-dependent autistic-behaviours in models of lysosomal storage disorders Nature communications 2021-06-09 [PMID: 34108486]
• K Goel, EL Beatman, N Egersdorf, A Scruggs, D Cao, EV Berdyshev, KS Schweitzer, I Petrache Sphingosine 1 Phosphate (S1P) Receptor 1 Is Decreased in Human Lung Microvascular Endothelial Cells of Smokers and Mediates S1P Effect on Autophagy Cells, 2021-05-14;10(5):. 2021-05-14 [PMID: 34068927]
• Sokhna Y, Mario R, Saray C et al. The parkinsonian LRRK2 R1441G mutation shows macroautophagy-mitophagy dysregulation concomitant with endoplasmic reticulum stress. Cell Biol Toxicol. 2021-05-31 [PMID: 34060004]
• Wojnarowicz PM, Escolano MG, Huang YH et al. Anti-tumor effects of an ID antagonist with no observed acquired resistance NPJ breast cancer 2021-05-24 [PMID: 34031428]
• OJ Sul, G Li, JE Kim, ES Kim, HS Choi 7-ketocholesterol enhances autophagy via the ROS-TFEB signaling pathway in osteoclasts: 7-KC in osteoclasts The Journal of nutritional biochemistry, 2021-05-21;0(0):108783. 2021-05-21 [PMID: 34023424]
• Charles T, Evelyn N, Craig C et al. p62/Sequestosome 1 regulates transforming growth factor beta signaling and epithelial to mesenchymal transition in A549 cells. Cell Signal. 2021-05-14 [PMID: 34000385]
• Jie L, Yanmei Q, Joshua C et al. CREG1 promotes lysosomal biogenesis and function. Autophagy. 2021-05-08 [PMID: 33966596]
• Shi M, Shepard S, Zhou Z et al. High Dietary Phosphate Exacerbates and Acts Independently of Low Autophagy Activity in Pathological Cardiac Remodeling and Dysfunction Cells 2021-04-01 [PMID: 33915953]
• Tamargo-GOmez I, MartInez-GarcIa GG, SuArez MF et al. ATG4D is the main ATG8 delipidating enzyme in mammalian cells and protects against cerebellar neurodegeneration Cell death and differentiation 2021-04-01 [PMID: 33795848]
• Joanna P, Andrew M, Christophe P et al. Altered Macrophage Function Associated with Crystalline Lung Inflammation in Acid Sphingomyelinase Deficiency. Am J Respir Cell Mol Biol. 2021-05-15 [PMID: 33662226]
• Y Mu, Y Maharjan, R Kumar Dutt, X Wei, JH Kim, J Son, C Park, R Park Pharmacological inhibition of catalase induces peroxisome leakage and suppression of LPS induced inflammatory response in Raw 264.7 cell PLoS ONE, 2021-02-19;16(2):e0245799. 2021-02-19 [PMID: 33606716]
• Robichaud S, Fairman G, Vijithakumar V et al. Identification of novel lipid droplet factors that regulate lipophagy and cholesterol efflux in macrophage foam cells Autophagy 2021-02-26 [PMID: 33590792]
• Lihua H, Andrew K, Scott H et al. DNAJB12 and Hsp70 Triage Arrested Intermediates of N1303K-CFTR for ER Associated-Autophagy. Mol Biol Cell. 2021-02-03 [PMID: 33534640]
• Recasens-Alvarez C, Alexandre C, Kirkpatrick J, et al. Ribosomopathy-associated mutations cause proteotoxic stress that is alleviated by TOR inhibition Nature cell biology 2021-01-25 [PMID: 33495632]
• Wang F, Zhang Y, Shen J, et al. The ubiquitin E3 ligase TRIM21 promotes hepatocarcinogenesis by suppressing the p62-Keap1-Nrf2 antioxidant pathway Cellular and molecular gastroenterology and hepatology 2021-01-19 [PMID: 33482392] (WB, IHC-P, ICC/IF, Mouse)
• Naho N, Chiaki I, Takako O et al. Essential role of autophagy in protecting neonatal haematopoietic stem cells from oxidative stress in a p62-independent manner. Sci Rep. 2021-01-18 [PMID: 33462315]
• Park J, Choi H, Kim YD, et al. Aberrant role of ALK in tau proteinopathy through autophagosomal dysregulation Molecular psychiatry 2021-01-15 [PMID: 33452442]
• Shouzi Z, Yuanyuan W, Lixin L et al. Case Report of a pathologically confirmed vascular parkinsonism with early cognitive impairment and Behavioral disturbance. BMC Neurol. 2021-01-11 [PMID: 33430818]
• Dong X, Yang Y, Zou Z et al. Sorting nexin 5 mediates virus-induced autophagy and immunity Nature 2020-12-16 [PMID: 33328639]
• Hyun-Young L, Seon-Hee O Autophagy-mediated cytoplasmic accumulation of p53 leads to apoptosis through DRAM-BAX in cadmium-exposed human proximal tubular cells. Biochem Biophys Res Commun. 2020-12-13 [PMID: 33321290]
• Zhao Y, Zou Z, Sun D et al. GLIPR2 is a negative regulator of autophagy and the BECN1-ATG14-containing phosphatidylinositol 3-kinase complex Autophagy 2020-11-23 [PMID: 33222586]
• Shireen S, Hetal S, Gil K et al. Loss of TAX1BP1-Directed Autophagy Results in Protein Aggregate Accumulation in the Brain. Mol Cell. 2020-11-17 [PMID: 33207181]
• Tara F, Chunxin W, Benjamin P et al. STING induces LC3B lipidation onto single-membrane vesicles via the V-ATPase and ATG16L1-WD40 domain. J Cell Biol. 2020-12-07 [PMID: 33201170]
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  Details:
  p62/SQSTM1 antibody used for WB on lysates of transversus abdominis muscle / TA muscles from mice that were fed with regular chow or fasted for 3-days (Figure 2B)
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Reviews for p62/SQSTM1 Antibody (H00008878-M01) (10)

Average Rating: 4.2

(Based on 10 reviews)

We have 10 reviews tested in 4 species: Human, Mouse, Rat, Drosophila.

reviewed by: Erika Geisbrecht Immunohistochemistry + Western Blot Drosophila 08/21/2023

Summary

• Application: Other
• Sample Tested: dissected carcass for muscle staining,drosophila larvae,drosophila larval muscle,whole drosophila larvae
• Species: Drosophila
• Lot: N7131-2C11

Comments

• Comments: ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.*** Drosophila muscle staining with the Novus antibody did not match the staining with a published antibody against drosophila ref(2)p (the drosophila equivalent of p62). Western blot with the Novus antibody did not show any bands with drosophila whole larval samples. Immunohistochemistry with fixed drosophila larval tissue Western blot with drosophila whole larval samples

reviewed by: Verified Customer ICC Human 01/24/2022

Summary

• Application: Immunocytochemistry
• Sample Tested: Hela cell line
• Species: Human
• Lot: 2C11

reviewed by: Verified Customer WB Human 08/17/2019

Summary

• Application: Western Blot
• Sample Tested: HEK293
• Species: Human
• Lot: H5191-2C11

Comments

• Comments: 60ug of total cell lysate was loaded for the KO screening. There were several non-specific bands below 50Kda region.

reviewed by: Verified Customer WB Mouse 12/18/2017

Summary

• Application: Western Blot
• Sample Tested: Mouse embryonic fibroblasts
• Species: Mouse
• Lot: H5191-2C11

Comments

• Comments: p62 knockout mouse embryonic fibroblasts
  Name: Anti-p62 antibody (H00008878-M01)
  Catalog #: Anti-p62 antibody (H00008878-M01)
  Lot Number: Anti-p62 antibody (H00008878-M01, Lot # H5191-2C11)
  PO/Order Number: Click here to enter text..
  WB Image Description (Please provide labels for all lanes): lane 1: WT mouse embryonic fibroblasts; lane 2: p62 knockout mouse embryonic fibroblasts
  Sample Information:
  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Cell Line or Tissue: mouse embryonic fibroblasts;
  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Species: mouse
  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Treatment: No treatment
  Lysate Preparation:
  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Date of lysate preparation: December 15, 2017
  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Lysis buffer used: 1X lysis buffer from Cell Signaling by adding PMSF
  Reducing agent: beta-mercaptoethanol, DTT
  If boiled (temperature/time): Yes
  Controls:
  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Positive Control: Wild type
  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Negative Control: Knockout
  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Loading Control (please attach additional images if applicable): No
  Protein Amount Loaded per lane: 20 ug
  Antibody Storage Conditions: -20℃
  Electrophoresis:
  Gel Percentage: 8%
  Electrophoresis Conditions: Tris-Glycine-SDS at room temperature
  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Voltage: 120V
  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Time: 2 hours
  Membrane Transfer:
  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Method (Submersion/Semi-dry): wet transfer
  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Membrane Type (PVDF/Nitrocellulose): Nitrocellulose
  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Time: 2 hours
  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Voltage: 100V
  Blocking:
  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Blocking Solution: 5% milk in 1X TBST
  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Time: 1 hour at room temperature
  Primary Antibody:
  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Dilution: 1/1000
  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Diluent Buffer: 2.5% BSA
  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Incubation Time: overnight
  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Incubation Temperature: 4℃
  Washing Conditions:
  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Wash Solution: 1X TBST
  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Time and Repetitions: 5 min each for 3 times
  
  Secondary Antibody
  Manufacturer and Catalog #: Promega, W402B, Lot # 0000191071
  Secondary description: goat anti-mouse secondary antibody
  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Dilution: 1/2000
  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Diluent Buffer: 3% milk
  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Incubation Time: 1 hour
  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Incubation Temperature: room temperature
  Detection Method:
  Detection: ECL (GE, cat # RPN2209, lot # 9838243)
  Procedure: Add equal volume of A and B, mix and apply on the membranes for 3-5 min before exposure
  Development Time: 20 seconds
  Molecular weight of band(s): ~ 65 kDa
  Experimental Concerns and Observations: A specific band around 65 kDa was observed

reviewed by: Woojong Lee WB Mouse 05/04/2017

Summary

• Application: Western Blot
• Sample Tested: DEndritic cell lysate
• Species: Mouse

reviewed by: Verified Customer IF Human 04/05/2016

Summary

• Application: Immunofluorescence
• Sample Tested: Differentiated cell line
• Species: Human

reviewed by: Fabrice GANKAM WB Rat 11/01/2014

Summary

• Application: Western Blot
• Sample Tested: Rat brain
• Species: Rat

reviewed by: Verified Customer WB Mouse 10/24/2014

Summary

• Application: Western Blot
• Sample Tested: mouse hepatocytes and HepG2 cells
• Species: Mouse

reviewed by: Nadine Bakkar IP Human 08/07/2014

Summary

• Application: Immunoprecipitation
• Sample Tested: Human spinal cord cytoplasmic homogenates
• Species: Human

reviewed by: Lin Zhang WB Mouse 09/28/2011

Summary

• Application: Western Blot
• Sample Tested: Mouse
• Species: Mouse

Bioinformatics

• Gene Symbol: SQSTM1
• Entrez:
  • 8878(Human)
  • 18412(Mouse)
• Uniprot:
  • Q13501()