pan Cadherin Antibody (S04-5A8)

Immunocytochemistry/Immunofluorescence: pan Cadherin Antibody (S04-5A8) [NBP3-15073] - Immunofluorescence of pan Cadherin (green) in MCF-7 using pan Cadherin antibody at dilution 1/5, and DAPI (blue)

Immunohistochemistry-Paraffin: pan Cadherin Antibody (S04-5A8) [NBP3-15073] - Immunohistochemistry of pan Cadherin in paraffin-embedded Human Cholangiocarcinoma using pan Cadherin Rabbit mAb at dilution 1/50

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP
• Clone: S04-5A8
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

pan Cadherin Antibody (S04-5A8) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human pan Cadherin (Uniprot#: P12830 P22223 P19022)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CDH1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunoprecipitation 0.0763888888888889
  • Western Blot 1:1000-1:5000
• Theoretical MW: 140 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for pan Cadherin Antibody (S04-5A8)

• Arc-1
• Cadherin (pan)
• cadherin 1, E-cadherin (epithelial)
• cadherin 1, type 1, E-cadherin (epithelial)
• cadherin-1
• calcium-dependent adhesion protein, epithelial
• CAM 120/80
• CD324 antigen
• CD324
• CDHE
• cell-CAM 120/80
• ECAD
• E-cadherin
• Epithelial cadherin
• LCAM
• UVOE-Cadherin
• uvomorulin

Background

Cadherins are members of a multigene family of single chain glycoprotein receptors mediating calcium dependent cell-cell adhesion. They play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Cadherins are expressed in a tissue specific manner and and are required for assembly of cells into solid tissue. Individual cadherin molecules are known to co-operate with each other to form a linear cell adhesion zipper. In adhesion junctions cadherins are bound to beta and gamma catenins which in turn bind to alpha catenin, an actin binding protein. Cadherins play an important part in tumor invasion and metastasis. This product is useful for the detection of members of the cadherin family or genetically engineered proteins containing the C-terminal cadherin tail, and for demonstration of adherens type cell-cell junctions regardless of their cadherin type.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDH1