PARP10 Antibody - BSA Free

Western Blot: PARP10 Antibody [NB100-2157] - Detection of Human PARP10 on HeLa whole cell lysate using NB100-2157. PARP10 was also immunoprecipitated using other rabbit anti-PARP10 antibodies.

Immunohistochemistry-Paraffin: PARP10 Antibody [NB100-2157] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti- PARP10 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PARP10 Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 300 and 350 of Poly (ADP-ribose) Polymerase Family, member 10 using the numbering given in entry NP_116178.1 (GeneID 84875)
• Localization: Nuclear
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PARP10
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500 -1:2000
  • Immunohistochemistry-Paraffin 1:500 -1:2000
  • Immunoprecipitation 1-4 ug/mg of lysate
  • Western Blot 1:500-1:2500
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PARP10 Antibody - BSA Free

• ARTD10
• EC 2.4.2.30
• FLJ14464
• PARP10
• PARP-10
• poly (ADP-ribose) polymerase family, member 10
• poly [ADP-ribose] polymerase 10

Background

PARP10 is a member of the poly(ADP-ribose) polymerase (PARP) family involved in a variety of processes which includes DNA metabolism, the DNA damage response, transcription and the maintenance of genomic stability. Although each member of the PARP family exhibits poly(ADP-ribose) polymerase activity which catalyzes the attachment of ADP-ribose units from NAD+ to substrate proteins, the endogenous substrates of many of the family members, including PARP10, remain to be identified. Recent studies have shown that PARP10 is localized to the nucleoli and associates with RNA polymerase II. PARP10 is a substrate of and is activated by CDK2 and may serve an important function in cell proliferation. Alternate names for PARP10 include poly(ADP-ribose) polymerase 10, PARP-10, and FLJ14464.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PARP10 Antibody (NB100-2157)(4)

Publications using NB100-2157

• Dhoonmoon A, Nicolae CM, Moldovan GL The KU-PARP14 axis differentially regulates DNA resection at stalled replication forks by MRE11 and EXO1 Nature communications 2022-08-27 [PMID: 36030235] (WB, KD, Human)
  
  Details:
  Supplementary Figure S1. H: Western blots confirming knockdowns of ZRANB3 and PARP10 in HeLa-BRCA2KO cells.
• Schleicher E M, Galvan A M et al. PARP10 promotes cellular proliferation and tumorigenesis by alleviating replication stress. Nucleic Acids Res 2018-09-28 [PMID: 30032250] (WB, Human)
• Shahrour MA, Nicolae CM, Edvardson S et al. PARP10 deficiency manifests by severe developmental delay and DNA repair defect Neurogenetics 2016-09-13 [PMID: 27624574] (WB, Human)
• Nicolae CM, Aho ER, Vlahos AH et al. The ADP-ribosyltransferase PARP10/ARTD10 interacts with Proliferating Cell Nuclear Antigen (PCNA) and is required for DNA damage tolerance. J Biol Chem 2014-04-07 [PMID: 24695737] (WB)

Bioinformatics

• Gene Symbol: PARP10
• Entrez:
  • 84875(Human)
• Uniprot:
  • NP_116178.1(Human)