PKA C (pan) [p Thr197] Antibody (JE45-17) - (Catalytic Subunit)

Western Blot: PKA C (pan) [p Thr197] Antibody (JE45-17) - (Catalytic Subunit) [NBP3-32788] - Western blot analysis of PKA C (pan) on different lysates with Rabbit anti-PKA C (pan) antibody (NBP3-32788) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: C6 cell lysate Lane 3: HeLa cell lysate Lane 4: HeLa serum starved overnight then treated with 100nM Calyculin A for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32788) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: JE45-17
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

PKA C (pan) [p Thr197] Antibody (JE45-17) - (Catalytic Subunit) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Thr197 of PKA C (pan). (Uniprot: P17612)
• Modification: p Thr197
• Localization: Cytoplasm, Cell membrane, Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PRKACA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PKA C (pan) [p Thr197] Antibody (JE45-17) - (Catalytic Subunit)

• CAFD1
• PKA C
• PKACA
• PPNAD4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKACA