PKC theta [p Thr219] Antibody (HL1157) - Azide and BSA Free

Western Blot: PKC theta [p Thr219] Antibody (HL1157) - Azide and BSA Free [NBP3-25607] - Untreated (-) and treated (+) Jurkat whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC theta (phospho Thr219) antibody [HL1157] (NBP3-25607) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: PKC theta [p Thr219] Antibody (HL1157) - Azide and BSA Free [NBP3-25607] - Untreated (-) and treated (+) Jurkat whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC theta (phospho Thr219) antibody [HL1157] (NBP3-25607) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Bv, RM
• Applications: WB, ICC/IF
• Clone: HL1157
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PKC theta [p Thr219] Antibody (HL1157) - Azide and BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Carrier-protein conjugated synthetic peptide surrounding phospho Thr219 of human PKC theta. The exact sequence is proprietary.
• Modification: p Thr219
• Predicted Species: Mouse (100%), Rat (100%), Rhesus Macaque (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PRKCQ
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Assay dependent
  • Western Blot 1:500-1:3000
• Theoretical MW: 82 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (84%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for PKC theta [p Thr219] Antibody (HL1157) - Azide and BSA Free

• EC 2.7.11
• EC 2.7.11.13
• MGC126514
• MGC141919
• nPKC-theta
• PKC theta
• PRKCQ
• PRKCT
• protein kinase C theta type
• protein kinase C, theta

Background

Members of the protein kinase C (PKC) family play a key regulatory role in variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (alpha, betaI, betaII and gamma) and novel (n) PKC isoforms (delta, episilon , zeta, eta and theta). Patterns of expression for each PKC isoform differs among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC delta and episilon are independent of Ca++. On the other hand, nPKC and episilon, as well as all of the cPKC members, possess phorbol ester-binding activities and kinase activities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKCQ