PKR Antibody (6H3A10) - BSA Free

Western Blot: PKR Antibody (6H3A10) [NBP2-37242] - Analysis using PKR mAb against (1) HEK293 and (2) PKR (AA: 329-551)-hIgGFc transfected HEK293 cell lysate.

Immunohistochemistry-Paraffin: PKR Antibody (6H3A10) [NBP2-37242] - Analysis of cervical cancer tissues using EIF2AK2 mouse mAb with DAB staining.

Flow Cytometry: PKR Antibody (6H3A10) [NBP2-37242] - Analysis of A431 cells using EIF2AK2 mouse mAb (green) and negative control (red).

Western Blot: PKR Antibody (6H3A10) [NBP2-37242] - Western blot analysis using EIF2AK2 mAb against human EIF2AK2 (AA: 329-551) recombinant protein. (Expected MW is 51.7 kDa)

Western Blot: PKR Antibody (6H3A10) [NBP2-37242] - Analysis using EIF2AK2 mouse mAb against A431 (1), THP-1 (2), MCF-7 (3), PC-12 (4) cell lysate.

Immunohistochemistry-Paraffin: PKR Antibody (6H3A10) [NBP2-37242] - Analysis of rectum cancer tissues using EIF2AK2 mouse mAb with DAB staining.

ELISA: PKR Antibody (6H3A10) [NBP2-37242] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu
• Applications: WB, ELISA, Flow, IHC, CyTOF-ready
• Clone: 6H3A10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

PKR Antibody (6H3A10) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human PKR (AA: 329-551) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: EIF2AK2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500 - 1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 62 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for PKR Antibody (6H3A10) - BSA Free

• double stranded RNA activated protein kinase
• EC 2.7.11.1
• eIF-2A protein kinase 2
• EIF2AK1
• EIF2AK2
• eukaryotic translation initiation factor 2-alpha kinase 2P1/eIF-2A protein kinase
• interferon-induced, double-stranded RNA-activated protein kinase
• interferon-inducible double stranded RNA dependent
• interferon-inducible elF2alpha kinase
• Interferon-inducible RNA-dependent protein kinase
• P68 Kinase
• PKR
• PKRp68 kinase
• PPP1R83
• PRKR
• PRKRMGC126524
• Protein Kinase R
• Protein kinase RNA-activated

Background

Protein kinase R (PKR) is a serine/threonine kinase that is induced by interferon and activated by double-stranded RNA. Upon activation, PKR is autophosphorylated at multiple sites and phosphorylates the

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PKR Antibody (NBP2-37242)(3)

Publications using NBP2-37242

• Gomez R, Tomatis C, Scalise M, Thomas P Yellow Fever Virus Infection Alters Mitochondrial Network Dynamics Authorea 2023-01-18 (Immunocytochemistry/ Immunofluorescence, Human)
• Tomatis C, León A, López Ortiz AO et al. Theiler's Murine Encephalomyelitis Virus Replicates in Primary Neuron Cultures and Impairs Spine Density Formation Neuroscience 2023-08-19 [PMID: 37598833]
• Lukhele S, Rabbo DA, Guo M et al. The transcription factor IRF2 drives interferon-mediated CD8+ T cell exhaustion to restrict anti-tumor immunity Immunity 2022-11-01 [PMID: 36370712]

Bioinformatics

• Gene Symbol: EIF2AK2