PKR Antibody (SC06-37)

Western Blot: PKR Antibody (SC06-37) [NBP2-67629] - Western blot analysis of PKR on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbi

Immunocytochemistry/Immunofluorescence: PKR Antibody (SC06-37) [NBP2-67629] - Staining PKR in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: PKR Antibody (SC06-37) [NBP2-67629] - Analysis of paraffin-embedded human kidney tissue using anti-PKR antibody. Counter stained with hematoxylin.

Western Blot: PKR Antibody (SC06-37) [NBP2-67629] - Analysis of PKR on different lysates using anti-PKR antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: Hela

Product Discontinued

PKR Antibody (SC06-37) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within human PKR aa 500-540. (SwissProt: P19525 Human)
• Localization: Nucleus, Cytoplasm, perinuclear region.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: EIF2AK2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Immunoprecipitation
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PKR Antibody (SC06-37)

• double stranded RNA activated protein kinase
• EC 2.7.11.1
• eIF-2A protein kinase 2
• EIF2AK1
• EIF2AK2
• eukaryotic translation initiation factor 2-alpha kinase 2P1/eIF-2A protein kinase
• interferon-induced, double-stranded RNA-activated protein kinase
• interferon-inducible double stranded RNA dependent
• interferon-inducible elF2alpha kinase
• Interferon-inducible RNA-dependent protein kinase
• P68 Kinase
• PKR
• PKRp68 kinase
• PPP1R83
• PRKR
• PRKRMGC126524
• Protein Kinase R
• Protein kinase RNA-activated

Background

Protein kinase R (PKR) is a serine/threonine kinase that is induced by interferon and activated by double-stranded RNA. Upon activation, PKR is autophosphorylated at multiple sites and phosphorylates the

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EIF2AK2