Poly-Ubiquitin Antibody - K48 Linkage - Azide and BSA Free

Dot Blot: Poly-Ubiquitin Antibody - K48 Linkage [NBP3-05659] - Dot-blot analysis of all sorts of peptides using Poly-Ubiquitin antibody (NBP3-05659) at 1:1000 dilution.

Western Blot: Poly-Ubiquitin Antibody - K48 Linkage [NBP3-05659] - Analysis of extracts of various cell lines, using K48-linkage Specific Polyubiquitin antibody at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 90s.

Western Blot: Poly-Ubiquitin Antibody - K48 Linkage [NBP3-05659] -Analysis of extracts of various cell lines, using K48-linkage Specific Polyubiquitin antibody at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 180s.

Product Discontinued

Poly-Ubiquitin Antibody - K48 Linkage - Azide and BSA Free Summary

• Immunogen: A synthetic peptide corresponding to a sequence containing polyubiquitinated protein (K48 linkage).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RPS27A
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Dot Blot 1:500 - 1:1000
  • Western Blot 1:100 - 1:500

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 50% glycerol, pH7.3.
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for Poly-Ubiquitin Antibody - K48 Linkage - Azide and BSA Free

• CEP80
• HEL112
• PolyUbiquitin
• Poly-Ubiquitin
• ribosomal protein S27a
• RPS27A
• S27A
• UBA80
• UBC
• UBCEP1
• UBCEP80

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RPS27A