PRDM10 Antibody

Product Discontinued

PRDM10 Antibody Summary

• Immunogen: Peptide with sequence C-NGNGSSEVHITKP corresponding to C-Terminus according to NP_064613.2, NP_955469.1, NP_955470.1, NP_955471.1.
• Localization: Nuclear.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PRDM10
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
• Application Notes: WB: No signal obtained yet but low background observed in human brain and human kidney lysates at up to 1 ug/ml.
• Control:

  PRDM10 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for PRDM10 Antibody

• KIAA1231PRDM zinc finger transcription factor
• MGC131802
• PFM7tristanin
• PR domain containing 10
• PR domain zinc finger protein 10
• PR domain-containing protein 10
• PR-domain family member 7
• TRIS
• Tristanin

Background

Similar to acetylation and phosphorylation, histone methylation at the N-terminal tail has emerged as an important role in regulating chromatin dynamics and gene activity. Histone methylation occurs on arginine and lysine residues and is catalyzed by two families of proteins, the protein arginine methyltransferase family and the SET-domain-containing methyltransferase family. Five members have been identified in the arginine methyltransferase family. About 27 are grouped into the SET-domain family, and another 17 make up the PR domain family that is related to the SET domain family. The retinoblastoma protein-interacting zinc finger gene RIZ 1 is a tumor suppressor gene and a FOUNDING member of the PR domain family. RIZ 1 inactivation is commonly found in many types of human cancers and occurs through loss of mRNA expression, frame shift mutation, chromosomal deletion, and missense mutation. RIZ 1 is also a tumor susceptibility gene in mice. The loss of RIZ 1 mRNA in human cancers was shown to associate with DNA methylation of its promoter CpG island. Methylation of the RIZ 1 promoter strongly correlated with lost or decreased RIZ 1 mRNA expression in breast, liver, colon, and lung cancer cell lines as well as in liver cancer tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PRDM10 Antibody (NB100-1136)(1)

Publication using NB100-1136

• Alvarez-Venegas R, Avramova Z. SET-domain proteins of the Su(var)3-9, E(z) and trithorax families. Gene 2002-02-20 [PMID: 12039029]

Bioinformatics

• Gene Symbol: PRDM10
• Entrez:
  • 56980(Human)
• Uniprot:
  • NP_064613.(Human)