Recombinant Mouse Prolactin Protein

SDS-Page: Mouse Prolactin Protein [NBP2-35120]

• Reactivity: Mu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Mouse Prolactin Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 197 amino acids corresponding to Prolactin Source: E. coli

  Uniprot ID: P06879

  Amino Acid Sequence: LPICSAGDCQ TSLRELFDRV VILSHYIHTL YTDMFIEFDK QYVQDREFMV KVINDCPTSS LATPEDKEQA LKVPPEVLLN LILSLVQSSS DPLFQLITGV GGIQEAPEYI LSRAKEIEEQ NKQLLEGVEK IISQAYPEAK GNGIYFVWSQ LPSLQGVDEE SKILSLRNTI RCLRRDSHKV DNFLKVLRCQ IAHQNNC
  
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  - 12 months from date of receipt, -20 to -70 °C as supplied.
  - 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  - 3 months, -20 to -70 °C under sterile conditions after reconstitution.

• Details of Functionality: Prolactin protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat Nb2-11 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 x10^6 IU/mg
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PRL
• Purity: > 98 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 0.1 EU/ug of Prolactin as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 22.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 98 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Mouse Prolactin Protein

• PRL
• Prolactin

Background

Prolactin, also named PRL and lactotrope is a peptide hormone, encoded by the PRL gene. The structure of prolactin is similar to that of growth hormone and placental lactogen. There are mainly three different forms of prolactin in regard to size: little prolactin, big prolactin and big big prolactin. Little prolactin is the predominant form. Although often associated with human milk production, prolactin plays a wide range of other roles in both humans and other verterbrates. Recombinant murine prolactin is a 22.4 kDa globular protein containing 197 amino acid residues and shares 77 % ~ 90 % a.a. sequence identity with human and rat prolactin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PRL
• Uniprot:
  • P06879(Mouse)