Ret [p Tyr905] Antibody

Western blot shows lysates of MDA-MB-468 human breast cancer cell line untreated (-) or treated (+) with 100 µM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Human Phospho-Ret (Y905) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3269), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Ret (Y905) at approximately 175 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

Ret [p Tyr905] Antibody Summary

• Immunogen: Phosphopeptide containing human Ret Y905 site
• Modification: p Tyr905
• Specificity: Detects human Ret when phosphorylated at Y905 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RET
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ret [p Tyr905] Antibody

• CDHF12MTC1
• CDHR16
• EC 2.7.10
• Hirschsprung disease 1
• Hirschsprung disease)
• MEN2A
• MEN2B
• multiple endocrine neoplasia and medullary thyroid carcinoma 1
• ret proto-oncogene
• RET transforming sequence
• Ret

Background

The GDNF family of neurotrophic factors forms a subfamily within the TGF-beta superfamily. These proteins are potent survival factors for various central and peripheral neurons during development and the adult animal. The GDNF family members (GDNF, neurturin, artemin and persephin) signal through multicomponent receptors that consist of the Ret receptor tyrosine kinase and one of four glycosyl-phosphatidylinositol (GPI)-linked ligand-binding subunits (GFR alpha -1 - 4). GFR alpha ‑1, ‑2, ‑3, and -4 are the preferred ligand-binding subunits for GDNF, neurturin, artemin and persephin, respectively. The Ret tyrosine-kinase receptor is encoded by the c-ret proto-oncogene. Mutations of the ret gene have been associated with various human diseases affecting tissues derived from the neural crest, including Hirschsprung’s disease, multiple endocrine neoplasia MEN2A and MEN2B, and familial medullary thyroid carcinoma. Human and mouse Ret share 83% amino acid sequence homology (77% homology in the extracellular domain and 93% homology in the cytoplasmic domain). Although Ret does not bind GDNF ligands directly, the extracellular domain of Ret binds the GDNF-GFR-alpha complex with high affinity and is a potent GDNF antagonist in the presence of soluble GFR-alpha (1 - 4).

1. Trupp, M. et al. (1998) Mol. Cell. Neurosci. 11:47.
2. Enokido, Y. et al. (1998) Curr. Biol. 8:1019.
3. Carlomagno, F. et al. (1998) Endocrinology 139:3613.
4. Baloh, R. et al. (1998) Neuron 21:1291.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ret Antibody (AF3269)(3)

Publications using AF3269

• Ito , Kenichir, Sakai , Katsuya, Suzuki , Yoshinor, Ozawa , Naoya, Hatta , Tomohisa, Natsume , Tohru, Matsumoto , Kunio, Suga , Hiroaki Artificial human Met agonists based on macrocycle scaffolds. Nat Commun, 2015-03-11;6(0):6373. 2015-03-11 [PMID: 25758345] (Western Blot, Human)
• Philip M. Spanheimer, Jung-Min Park, Ryan W. Askeland, Mikhail V. Kulak, George W. Woodfield, James P. De Andrade et al. Inhibition of RET Increases the Efficacy of Anti-Estrogen and is a Novel Treatment Strategy for Luminal Breast Cancer Clinical Cancer Research 4/15/2014 [PMID: 24526731]
• Cavel O, Shomron O, Shabtay A, Vital J, Trejo-Leider L, Weizman N, Krelin Y, Fong Y, Wong R, Amit M, Gil Z Endoneurial macrophages induce perineural invasion of pancreatic cancer cells by secretion of GDNF and activation of RET tyrosine kinase receptor. Cancer Res, 2012-09-12;72(22):5733-43. 2012-09-12 [PMID: 22971345] (Western Blot, Human)

Bioinformatics

• Gene Symbol: RET