SALL2 Antibody - BSA Free

Immunohistochemistry-Paraffin: SALL2 Antibody [NBP2-13276] - Staining of human cerebellum shows moderate nuclear positivity, mainly in cells in granular layer.

Immunohistochemistry-Paraffin: SALL2 Antibody [NBP2-13276] - Staining of human liver shows no positivity in hepatocytes as expected.

Immunohistochemistry-Paraffin: SALL2 Antibody [NBP2-13276] - Staining of human cerebral cortex shows moderate nuclear positivity in glial cells.

Immunohistochemistry-Paraffin: SALL2 Antibody [NBP2-13276] - Staining of human tonsil shows no postivity in non-germinal center cells as expected.

Western Blot-SALL2 Antibody [NBP2-13276] - Analysis in human cell lines HEK293 and U-251MG using Anti-SALL2 antibody. Corresponding SALL2 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SALL2 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to the amino acids: FLAHQNACSTDPPVMVIIGGQENPNNSSASSEPRPEGHNNPQVMDTEHSNPPDSGSSVPTDPTWGPERRGEESSGHFLVAATGTAAGGGGGLILASPKLGATPLPPESTPA
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SALL2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  SALL2 Protein (NBP2-13276PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (88%). Mouse reactivity reported in scientific literature (PMID: 26181197).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for SALL2 Antibody - BSA Free

• FLJ55746
• Hsal2
• KIAA0360sal (Drosophila)-like 2
• p150(Sal2)
• SAL2
• Sal-2
• sal-like 2 (Drosophila)
• sal-like protein 2
• Zinc finger protein 795
• Zinc finger protein SALL2
• Zinc finger protein Spalt-2
• ZNF795FLJ10414

Background

Probable transcription factor

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SALL2 Antibody (NBP2-13276)(2)

Publications using NBP2-13276

• Burns JC, Kelly MC, Hoa M et al. Single-cell RNA-Seq resolves cellular complexity in sensory organs from the neonatal inner ear. Nat Commun 2015-10-15 [PMID: 26469390] (IF/IHC, Mouse)
• Escobar D, Hepp MI, Farkas C et al. Sall2 is required for proapoptotic Noxa expression and genotoxic stress-induced apoptosis by doxorubicin. Cell Death Dis 2015-07-16 [PMID: 26181197] (WB, Mouse, Human)

Bioinformatics

• Gene Symbol: SALL2