SDHA Antibody (SDHA/7493) - Azide and BSA Free

Formalin-fixed, paraffin-embedded human colon carcinoma stained with SDHA Antibody (SDHA/7493) - Azide and BSA Free. Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human kidney stained with SDHA Antibody (SDHA/7493) - Azide and BSA Free. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

• Reactivity: Hu
• Applications: IHC
• Clone: SDHA/7493
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

SDHA Antibody (SDHA/7493) - Azide and BSA Free Summary

• Description: Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
• Immunogen: Recombinant fragment (around aa 450-650) of human SDHA protein (exact sequence is proprietary)
• Localization: Mitochondrion inner membrane
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Gene: SDHA
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2ug/ml for 30 minutes at RT
• Application Notes: Immunohistochemistry-Paraffin 1-2ug/ml for 30 minutes at RT. Optimal dilution for a specific application should be determined.. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for SDHA Antibody (SDHA/7493) - Azide and BSA Free

• CMD1GG
• flavoprotein subunit of complex II
• FP
• MC2DN1
• NDAXOA
• PGL5
• PPGL5
• SDH1
• SDH2
• SDHA
• SDHF
• succinate dehydrogenase complex, subunit A, flavoprotein (Fp)

Background

The SDHA gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SDHA