Secretory phospholipase A2 Type V Antibody (3G1)

Immunofluorescence: Secretory phospholipase A2 Type V Antibody (3G1) [NB100-74433] - IF analysis using this antibody.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clone: 3G1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Secretory phospholipase A2 Type V Antibody (3G1) Summary

• Immunogen: Full length W79A human group V phospholipase A2.
• Specificity: Group V Secretory Phospholipase A2 (3G1)
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PLA2G5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:1000
• Application Notes: WB: Detects an approx. 15 kDa band representing Group V Secretory Phospholipase A2 from mouse macrophages.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 10868798). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Secretory phospholipase A2 Type V Antibody (3G1)

• Ca2+-dependent phospholipase A2
• calcium-dependent phospholipase A2
• DKFZp686C2294
• EC 3.1.1.4
• Group V phospholipase A2
• GV-PLA2
• hVPLA(2)
• MGC46205
• Phosphatidylcholine 2-acylhydrolase 5
• phospholipase A2, group V
• PLA2-10

Background

Secretory phospholipase A2 Type V (PLA2) catalyzes the hydrolysis of fatty acids at the sn-2 position of glycerophospholipids and is one of the isoforms in the growing list of the PLA2 enzyme family. This enzyme, rather than sPLA2 (Type II), is responsible for arachidonic acid mobilization leading to prostaglandin production in macrophages and mast cells. Consistent with this role, sPLA2 (Type V) is associated with the golgi apparatus, nuclear envelope, and plasma membrane in murine bone marrow-derived mast cells. sPLA2 (Type V) has been cloned from a variety of species including human, mouse and rat. The enzyme is expressed in heart, lung, placenta and spleen, as well as P388D1 macrophages and mast cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PLA2G5
• Entrez:
  • 18784(Mouse)